Journal of Applied Phycology

, Volume 31, Issue 2, pp 1455–1465 | Cite as

Red seaweed Pyropia columbina as antioxidant supplement in feed for cultured juvenile Pacú (Piaractus mesopotamicus)

  • Raúl E. CianEmail author
  • Carla Bacchetta
  • Andrea Rossi
  • Jimena Cazenave
  • Silvina R. Drago


The aim of this study was to evaluate the antioxidant effect of red seaweed Pyropia columbina on juvenile Pacú (Piaractus mesopotamicus). Two hundred and ten (n = 210) fish were fed for 90 days with a control extruded feed (CEF) or the same feed added with P. columbina (35 g kg−1) (PcEF). No significant difference in final body weight, specific growth rate, condition factor, and liver somatic index was detected between dietary treatments (p > 0.05). Fish fed with PcEF showed lower lipid peroxidation (28.2 ± 2.9 vs. 39.4 ± 3.7 mmol malondialdehyde mg−1 protein) and superoxide dismutase (23.3 ± 2.3 vs. 37.7 ± 3.0 U mg−1 protein) and reduced SOD/CAT ratio (5.5 ± 0.3 vs. 8.1 ± 0.7) in intestine than those fed with CEF (p < 0.05). These results also were observed in liver and white muscle, indicating a systemic effect. No difference in glutathione S-transferase and catalase (CAT) activity in intestine, liver, and white muscle was detected between dietary treatments (p > 0.05). PcEF showed higher hemoglobin (7.5 ± 0.4 vs. 6.6 ± 0.3 g 100 mL−1) and mean corpuscular hemoglobin concentration (18.4 ± 1.8 vs. 25.7 ± 2.7%) than those fed with CEF diet (p < 0.05). Beneficial effects on lipid metabolism were observed in fish fed with PcEF respect to control diet. Pyropia columbina could be used as a natural antioxidant ingredient in fish feeding contributing a better nutritional status of cultured fish.


In vivo antioxidant effect Red seaweed Piaractus mesopotamicus Extruded fish feed 



All authors read and approved the final manuscript.

Funding information

This work was funded by the projects PICT-2013-1804 and CAI + D 2011 PI 0292 LI from ANPCyT and Universidad Nacional del Litoral, respectively.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.


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© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Instituto de Tecnología de Alimentos, CONICET, FIQ-UNLSanta FeArgentina
  2. 2.Instituto Nacional de Limnología, CONICET-UNLSanta FeArgentina

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