Full-length transcriptome sequences obtained by a combination of sequencing platforms applied to heat shock proteins and polyunsaturated fatty acids biosynthesis in Pyropia haitanensis
Pyropia haitanensis is a high-yield commercial seaweed in China. Pyropia haitanensis farms often suffer from problems such as severe germplasm degeneration, while the mechanisms underlying resistance to abiotic stresses remain unknown because of lacking genomic information. Although many previous studies focused on using next-generation sequencing (NGS) technologies, the short-read sequences generated by NGS generally prevent the assembly of full-length transcripts, and then limit screening functional genes. In the present study, which was based on hybrid sequencing (NGS and single-molecular real-time sequencing) of the P. haitanensis thallus transcriptome, we obtained high-quality full-length transcripts with a mean length of 2998 bp and an N50 value of 3366 bp. A total of 14,773 unigenes (93.52%) were annotated in at least one database, while approximately 60% of all unigenes were assembled by short Illumina reads. Moreover, we herein suggested that the genes involved in the biosynthesis of polyunsaturated fatty acids and heat shock proteins play an important role in the process of development and resistance to abiotic stresses in P. haitanensis. The present study, together with previously published ones, may facilitate seaweed transcriptome research.
KeywordsPyropia haitanensis Next-generation sequencing Single-molecule real-time transcript sequencing Polyunsaturated fatty acids Heat shock proteins
This work was supported by the National Natural Science Foundation of China (Grant No: 41276177), and Supported by China Agriculture Research System (CARS-50), and the Natural Science Foundation of Fujian, China (Grant Nos. 2014J07006 and 2014J05041). The authors thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript.
Compliance with ethical standards
The authors declare that they have no competing interests.
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