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Journal of Applied Phycology

, Volume 31, Issue 2, pp 1185–1196 | Cite as

Proteomic analysis of the salt-adapted and directly salt-(NaCl and NaCl+Na2SO4 mixture) stressed cyanobacterium Anabaena fertilissima

  • Ashwani K. RaiEmail author
  • Prashant Swapnil
Article

Abstract

Salinity is a serious threat to agriculture productivity. Beneficial microbes could be simple and low-cost biological methods to mitigate the salt toxicity. The present study describes proteome dynamics and salt stress tolerance of the salt-primed (pre-exposed to salt) and directly salt-stressed cyanobacterium Anabaena fertilissima. The difference in the proteome of differently salt-treated and the control cells was the abundance of proteins. Out of 130 proteins resolved in control, 51.8% remained constant, 25.5% were upregulated, and 22.7% protein spots were downregulated in salt-adapted cells (exposed to 500 mM NaCl). However, in cells exposed to 250 mM NaCl, percentage of constant, upregulated, and downregulated proteins was 56.8, 16, and 27.2, whereas in the cells exposed to equimolar NaCl+Na2SO4 mixture, these values were 41.8, 29.4, and 28.7, respectively. This indicated that an altered protein expression occurred in large number of protein species under different salt-stress regimes. Four proteins showing significant and reproducible changes under salt stress showed close homology with photosystem I reaction center subunit XII, epoxyqueuosine reductase, response regulator protein VraR, and molybdopterin biosynthesis protein. The kinetic analysis revealed that salt treatment increased the abundance of all the four identified proteins. Despite the exposure to higher NaCl concentration (500 mM), salt-adapted cells showed minimal hyper accumulation of these four proteins, followed by NaCl and NaCl+Na2SO4 mixture. Lesser accumulation of these identified proteins in salt mixture stressed cells than that of NaCl stressed cells revealed that the availability of sulfur during salt-stress relieved the salt-toxicity, while Cl increased it.

Keywords

Protein abundance Salt acclimation Salt mixture Cyanobacterium Pre-exposure to salt 

Notes

Acknowledgements

Ashwani K Rai gratefully acknowledges the National Academy of Sciences, India, for awarding NASI-Senior Scientist Fellowship.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10811_2018_1607_MOESM1_ESM.docx (3 mb)
ESM 1 (DOCX 3039 kb)

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© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Department of BotanyBanaras Hindu UniversityVaranasiIndia

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