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Co-cultivation of siderophore-producing bacteria Idiomarina loihiensis RS14 with Chlorella variabilis ATCC 12198, evaluation of micro-algal growth, lipid, and protein content under iron starvation

  • Soundarya Rajapitamahuni
  • Pooja Bachani
  • Raj Kumar Sardar
  • Sandhya Mishra
Article

Abstract

Co-cultivation systems offer the potential to commercialize microalgae biomass. The key purpose of the study was to understand the relationship between siderophore-producing bacterium Idiomarina loihiensis RS14 and Chlorella variabilis ATCC 12198 strain for Chlorella growth enhancement. After observing growth enhancement in C. variabilis by adding metal chelator deferroxamine mesylate (siderophore standard) and purified siderophore from I. loihiensis (1 mg mL−1), a co-cultivation system was designed where axenic microalgae and co-cultured (microalgae + bacteria) aliquots were grown in (1:9, 9:1, 1:1) volumetric inoculum ratio (mL) under iron-sufficient and iron-deficient conditions. The co-culture volumetric ratio 1:1 (microalgae/bacteria) showed bleaching of microalgae and 1:9 showed less biomass (310 mg L−1) comparatively with 9:1 that increased 35% of biomass, i.e., 650 mg L−1 (axenic) to 1000 mg L−1 (co-cultured) in iron-deficient media. The inoculum ratios were optimized in 100 mL shake flask and 9:1 ratio was further scaled up with the similar conditions, and the co-culture showed 20% increase in biomass, i.e., 285.6 mg L−1 (axenic) to 356 mg L−1 (co-cultured). The co-cultured biomass contains 19.70% lipid content compared with axenic algae, i.e., 18.41% which shows 7% of increase in co-culture. Protein content increased to 30% in co-culture microalgae compared with axenic microalgae. Scanning electron microscope images show crumpled surface of Chlorella cells in co-cultured compared with its axenic cells. This finding is of interest for biofuel production from microalgae, often attained through nutrient-starvation processes leading to oil accumulation.

Keywords

Co-cultivation Micro-algal growth enhancement Iron starvation Siderophore Lipid 

Notes

Acknowledgements

We are thankful to Ms. Khushbu Bhayani, Dr. Kaumeel Chokshi, and Dr. Sourish Bhattacharya for their timely help. We are also thankful to Mr. Jayesh Chaudhary for SEM analysis.

Funding information

CSIR-CSMCRI Registration Number PRIS 098/2017 has been assigned to the manuscript. RS acknowledges DST for her INSPIRE funding support. PB acknowledges CSIR-SRF for the funding support. RS, PB, and RKS acknowledge AcSIR for their Ph.D. enrolment.

Supplementary material

10811_2018_1591_MOESM1_ESM.docx (2.5 mb)
ESM 1 (DOCX 2553 kb)

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Soundarya Rajapitamahuni
    • 1
    • 2
  • Pooja Bachani
    • 1
    • 2
  • Raj Kumar Sardar
    • 1
    • 2
  • Sandhya Mishra
    • 1
    • 2
  1. 1.Division of Biotechnology and PhycologyCentral Salt and Marine Chemicals Research Institute (CSIR)BhavnagarIndia
  2. 2.Academy of Scientific and Innovative Research (AcSIR)Central Salt and Marine Chemicals Research Institute (CSIR)BhavnagarIndia

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