Effects of Xanthophyllomyces dendrorhous on cell growth, lipid, and astaxanthin production of Chromochloris zofingiensis by mixed culture strategy
The alga Chromochloris zofingiensis and the yeast Xanthophyllomyces dendrorhous are typical microorganisms which can accumulate high-value astaxanthin and lipid simultaneously. This study investigated the synergistic effects of X. dendrorhous on the cell growth, lipid, and astaxanthin production of C. zofingiensis by a mixed culture approach. Compared to the pure culture of C. zofingiensis, enhanced lipid and astaxanthin production were obtained in the mixed culture. The maximum astaxanthin and lipid yield achieved in the mixed culture with the ratio of 3:1 (algae to yeast) were 5.50 mg L−1 and 2.37 g L−1, respectively, which were 1.10- and 2.72-fold that of C. zofingiensis monoculture. Additionally, lipid obtained from the mixed culture had a plant oil-like fatty acid composition. This study provides a new insight into the integration of natural astaxanthin production with microbial lipid.
KeywordsLipid Astaxanthin Mixed culture Yeast Alga
This work was funded by the Science and Technology Program of Guangdong (Grant nos. 2016A010105001 and 2015A20216003), the Sciences and Technology of Guangzhou (Grant no. 201704030084), the Science and Technology Program in Marine and Fishery of Guangdong (Grant no. A201401C01).
- Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Physiol Pharmacol 37:911–917Google Scholar
- Liu JS, Z.; Zhong, Y., Gerken H, Huang J, Chen F (2013) Utilization of cane molasses towards cost-saving astaxanthin production by a Chlorella zofingiensis mutant. J Appl Phycol 25:1447–1456Google Scholar
- Liu L, Chen J, Lim P-E, Wei D (2018) Dual-species cultivation of microalgae and yeast for enhanced biomass and microbial lipid production. J Appl Phycol. https://doi.org/10.1007/s10811-018-1526-y
- Mulders KJM, Janssen JH, Martens DE, Wijffels RH, Lamers PP (2014) Effect of biomass concentration on secondary carotenoids and triacylglycerol (TAG) accumulation in nitrogen-depleted Chlorella zofingiensis. Algal Res 6, Part A:8–16Google Scholar