Journal of Applied Phycology

, Volume 30, Issue 6, pp 3009–3015 | Cite as

Effects of Xanthophyllomyces dendrorhous on cell growth, lipid, and astaxanthin production of Chromochloris zofingiensis by mixed culture strategy

  • Xueya Jiang
  • Lu Liu
  • Junhui Chen
  • Dong WeiEmail author
8th Asian Pacific Phycological Forum


The alga Chromochloris zofingiensis and the yeast Xanthophyllomyces dendrorhous are typical microorganisms which can accumulate high-value astaxanthin and lipid simultaneously. This study investigated the synergistic effects of X. dendrorhous on the cell growth, lipid, and astaxanthin production of C. zofingiensis by a mixed culture approach. Compared to the pure culture of C. zofingiensis, enhanced lipid and astaxanthin production were obtained in the mixed culture. The maximum astaxanthin and lipid yield achieved in the mixed culture with the ratio of 3:1 (algae to yeast) were 5.50 mg L−1 and 2.37 g L−1, respectively, which were 1.10- and 2.72-fold that of C. zofingiensis monoculture. Additionally, lipid obtained from the mixed culture had a plant oil-like fatty acid composition. This study provides a new insight into the integration of natural astaxanthin production with microbial lipid.


Lipid Astaxanthin Mixed culture Yeast Alga 


Funding information

This work was funded by the Science and Technology Program of Guangdong (Grant nos. 2016A010105001 and 2015A20216003), the Sciences and Technology of Guangzhou (Grant no. 201704030084), the Science and Technology Program in Marine and Fishery of Guangdong (Grant no. A201401C01).


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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.School of Food Sciences and EngineeringSouth China University of TechnologyGuangzhouPeople’s Republic of China

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