Journal of Applied Phycology

, Volume 30, Issue 6, pp 2989–2996 | Cite as

Effect of crude glycerol on heterotrophic growth of Chlorella pyrenoidosa and Coccomyxa subellipsoidea C-169

  • Zongyi Yu
  • Lu Liu
  • Junhui Chen
  • Xinpeng Wang
  • Dong WeiEmail author
8th Asian Pacific Phycological Forum


The feasibility of utilizing crude glycerol as carbon source for heterotrophic growth of the green microalgae Chlorella pyrenoidosa and Coccomyxa subellipsoidea C-169 was investigated. The highest biomass concentration of C. pyrenoidosa (6.25 g L−1) and C. subellipsoidea C-169 (7.62 g L−1) was achieved in basal medium containing 5 and 10 g L−1crude glycerol. Compared to pure glycerol and glucose, the algal cells grown in crude glycerol media obtained a higher intracellular protein content, while the microalgal lipid consists of a large amount of unsaturated fatty acids. The most abundant fatty acids in C. pyrenoidosa and C. subellipsoidea C-169 were linolenic acid (C18:3) and palmitic acid (16:0). The cetane number (49.0) of both investigated algal species corresponds to the requirements of the Standard B100. Our results indicate that crude glycerol could provide a promising alternative feedstock for heterotrophic growth of these two microalgae.


Chlorophyceae Microalgae Crude glycerol Heterotrophic growth Biodiesel 


Funding information

This work was funded by the program of Sciences and Technology of Guangzhou (Grant No. 201704030084), the Science and Technology Program in Marine and Fishery of Guangdong (Grant No. A201401C01), and the Science and Technology Program of Guangdong (Grant Nos. 2015A020216003, 2016A010105001).


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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Zongyi Yu
    • 1
  • Lu Liu
    • 1
  • Junhui Chen
    • 1
  • Xinpeng Wang
    • 1
  • Dong Wei
    • 1
    Email author
  1. 1.School of Food Science and EngineeringSouth China University of TechnologyGuangzhouPeople’s Republic of China

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