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Journal of Applied Phycology

, Volume 30, Issue 6, pp 3483–3492 | Cite as

Heterotrophic growth and oil production from Micractinium sp. ME05 using molasses

  • Iskin Kose Engin
  • Deniz Cekmecelioglu
  • Ayse Meral Yücel
  • Huseyin Avni OktemEmail author
Article

Abstract

In this study the thermo-resistant green alga Micractinium sp. ME05 was cultivated in media containing molasses as a carbon source. Shake flask experiments and 2-L bioreactor experiments were conducted at different inoculum ratios, aeration rates, and agitation speeds. The experimental condition which resulted in the highest biomass concentration (3.73 ± 0.45 g L−1) with 10% inoculum in 500-mL flasks was scaled up to 2-L flasks at two aeration rates (0.25 and 0.5 L min−1). An increase in biomass concentration from 2.35 ± 0.53 to 3.06 ± 0.21 g L−1 was observed with an increase of aeration rate from 0.25 to 0.50 L min−1, which demonstrated significant effect of aeration rate on biomass concentration (p = 0.000 < 0.05). In 2-L bioreactor experiments, highest biomass productivity (0.53 ± 0.076 g L−1 day−1) and lipid productivity (7.7 ± 1.6 g L−1 day−1) were obtained with 5% (v/v) inoculum and 50 rpm agitation speed. The principal fatty acids were palmitic acid (C16:0) and linoleic acid (C18:2) comprising 30.2 ± 1.01 and 45.2 ± 1.32% of the total fatty acid content, respectively. Thus, the present study highlights the possibility of using molasses for biomass and lipid production with Micractinium sp. ME05 under different cultivation conditions. Using low cost feedstock such as molasses would be valuable in terms of evaluating waste materials for further biodiesel production.

Keywords

Chlorophyta Micractinium sp. Molasses Heterotrophic growth Bioreactor 

Notes

Acknowledgements

This study was carried out at the Middle East Technical University (METU) Biology Department Plant Biotechnology Laboratory and METU Food Engineering Department Bioprocess Laboratory. We would like to thank Asst. Prof. Dr. Melih Onay for his isolation and characterization of microalgal species used in this study.

Funding information

This study was funded by the Scientific and Technological Research Council of Turkey (TUBITAK) Project Number 114Z487).

Supplementary material

10811_2018_1486_MOESM1_ESM.docx (14 kb)
ESM 1 (DOCX 13 kb)

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Iskin Kose Engin
    • 1
    • 2
  • Deniz Cekmecelioglu
    • 3
  • Ayse Meral Yücel
    • 1
    • 4
  • Huseyin Avni Oktem
    • 1
    • 4
    • 5
    Email author
  1. 1.Department of BiotechnologyMiddle East Technical UniversityAnkaraTurkey
  2. 2.Central Laboratory, Molecular Biology and BiotechnologyMiddle East Technical UniversityAnkaraTurkey
  3. 3.Departmenf of Food EngineeringMiddle East Technical UniversityAnkaraTurkey
  4. 4.Department of Biological SciencesMiddle East Technical UniversityAnkaraTurkey
  5. 5.Nanobiz Ltd. METU-TechnopolisAnkaraTurkey

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