Journal of Applied Phycology

, Volume 30, Issue 6, pp 3223–3232 | Cite as

The potential of fucoidans from Chnoospora minima and Sargassum polycystum in cosmetics: antioxidant, anti-inflammatory, skin-whitening, and antiwrinkle activities

  • I. P. Shanura Fernando
  • K. K. Asanka Sanjeewa
  • Kalpa W. Samarakoon
  • Hyun-Soo Kim
  • U. K. D. S. S. Gunasekara
  • Young-Jin Park
  • D. T. U. Abeytunga
  • Won Woo LeeEmail author
  • You-Jin JeonEmail author
8th Asian Pacific Phycological Forum


Over the years, bioactive and biocompatible natural products have received increased attention as ingredients of cosmeceutical formulations owing to their robust properties compared to many of the synthetic chemicals in use. Many natural products derived from algae have shown promising cosmetizing properties. Seaweed polysaccharides, in particular, have received increased attention for their biofunctional and physicochemical characteristics. This study aimed to design a cost-effective strategy to purify fucoidans from the untapped brown algae Chnoospora minima (CMF) and Sargassum polycystum (SPF) harvested in Sri Lanka and to evaluate their cosmetizing properties. Based on Fourier transform infrared (FTIR) spectroscopy and monosaccharide composition analysis, the purified polysaccharides were rich in fucoidan. Relatively high sulfate content was detected in both fucoidans. Experiments were carried out to evaluate antioxidant, UV-protective, anti-inflammatory, antiwrinkling, and skin-whitening effects. Both CMF and SPF showed 2,2-diphenyl-1-picrylhydrazyl (DPPH) and alkyl radical-scavenging activities, anti-inflammatory effects on lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages, considerable collagenase and elastase inhibitory properties and skin-whitening effects via direct inhibition of tyrosinase, and intracellular melanin synthesis indicating promising cosmetizing effects.


Phaeophyta Fucoidan Chnoospora minima Sargassum polycystum Whitening Antiwrinkling 



This research was supported by a grant from the Marine Biotechnology Program (20170488) funded by the Ministry of Oceans and Fisheries, Korea.


  1. Aguilar-Briseño J, Cruz-Suarez L, Sassi J-F, Ricque-Marie D, Zapata-Benavides P, Mendoza-Gamboa E, Rodríguez-Padilla C, Trejo-Avila L (2015) Sulphated polysaccharides from Ulva clathrata and Cladosiphon okamuranus seaweeds both inhibit viral attachment/entry and cell-cell fusion, in NDV infection. Mar Drugs 13:697–712CrossRefGoogle Scholar
  2. Berteau O, Mulloy B (2003) Sulfated fucans, fresh perspectives: structures, functions, and biological properties of sulfated fucans and an overview of enzymes active toward this class of polysaccharide. Glycobiology 13:29R–40R 240CrossRefGoogle Scholar
  3. Cha S-H, Ko S-C, Kim D, Jeon Y-J (2011) Screening of marine algae for potential tyrosinase inhibitor: those inhibitors reduced tyrosinase activity and melanin synthesis in zebrafish. J Dermatol 38:354–363CrossRefGoogle Scholar
  4. Chandler SF, Dodds JH (1983) The effect of phosphate, nitrogen and sucrose on the production of phenolics and solasodine in callus cultures of Solanum laciniatum. Plant Cell Rep 2:205–208CrossRefGoogle Scholar
  5. Cho D, Seung Kang J, Hoon Park J, Kim Y-I, Hahm E, Lee J, Yang Y, Jeon J, Song H, Park H, Kim T, Pang S, Kim C-W, Il Hwang Y, Jae Lee W (2002) The enhanced IL-18 production by UVB irradiation requires ROI and AP-1 signaling in human keratinocyte cell line (HaCaT). Biochem Biophys Res Commun 298:289–295CrossRefGoogle Scholar
  6. Dodgson KS, Price RG (1962) A note on the determination of the ester sulphate content of sulphated polysaccharides. Biochem J 84:106–110CrossRefGoogle Scholar
  7. DuBois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356CrossRefGoogle Scholar
  8. Dubois RN, Abramson SB, Crofford L, Gupta RA, Simon LS, Van De Putte LB, Lipsky PE (1998) Cyclooxygenase in biology and disease. FASEB J 12:1063–1073CrossRefGoogle Scholar
  9. Dzul JC, Puc RM, Robledo D, Pelegrín YF (2015) Hepatoprotective effect of the fucoidan from the brown seaweed Turbinaria tricostata. J Appl Phycol 27:2123–2135CrossRefGoogle Scholar
  10. Fernando IPS, Kim H-S, Sanjeewa KKA, Oh J-Y, Jeon Y-J, Lee WW (2017a) Inhibition of inflammatory responses elicited by urban fine dust particles in keratinocytes and macrophages by diphlorethohydroxycarmalol isolated from a brown alga Ishige okamurae. Algae 32:261–273CrossRefGoogle Scholar
  11. Fernando IPS, Sanjeewa KKA, Samarakoon KW, Lee WW, Kim H-S, Kim E-A, Gunasekara UKDSS, Abeytunga DTU, Nanayakkara C, de Silva ED, Lee H-S, Jeon Y-J (2017b) FTIR characterization and antioxidant activity of water soluble crude polysaccharides of Sri Lankan marine algae. Algae 32:75–86CrossRefGoogle Scholar
  12. Fitton JH (2011) Therapies from fucoidan; multifunctional marine polymers. Mar Drugs 9:1731–1760CrossRefGoogle Scholar
  13. Fitton JH, Dell’Acqua G, Gardiner V-A, Karpiniec SS, Stringer DN, Davis E (2015) Topical benefits of two fucoidan-rich extracts from marine macroalgae. Cosmetics 2:66–81CrossRefGoogle Scholar
  14. Guerrero P, Etxabide A, Leceta I, Peñalba M, de la Caba K (2014) Extraction of agar from Gelidium sesquipedale (Rhodophyta) and surface characterization of agar based films. Carbohydr Polym 99:491–498CrossRefGoogle Scholar
  15. Heo S-J, Ko S-C, Cha S-H, Kang D-H, Park H-S, Choi Y-U, Kim D, Jung W-K, Jeon Y-J (2009) Effect of phlorotannins isolated from Ecklonia cava on melanogenesis and their protective effect against photo-oxidative stress induced by UV-B radiation. Toxicol In Vitro 23:1123–1130CrossRefGoogle Scholar
  16. Heo S-J, Ko S-C, Kang S-M, Cha S-H, Lee S-H, Kang D-H, Jung W-K, Affan A, Oh C, Jeon Y-J (2010) Inhibitory effect of diphlorethohydroxycarmalol on melanogenesis and its protective effect against UV-B radiation-induced cell damage. Food Chem Toxicol 48:1355–1361CrossRefGoogle Scholar
  17. Horwitz W (2002) Instructions for inserting: official methods of analysis of AOAC International. AOAC InternationalGoogle Scholar
  18. Jung H-Y, Shin J-C, Park S-M, Kim N-R, Kwak W, Choi B-H (2014) Pinus Densiflora extract protects human skin fibroblasts against UVB-induced photoaging by inhibiting the expression of MMPs and increasing type I procollagen expression. Toxicol Rep 1:658–666CrossRefGoogle Scholar
  19. Kammeyer A, Luiten RM (2015) Oxidation events and skin aging. Ageing Res Rev 21:16–29CrossRefGoogle Scholar
  20. Kang M-C, Kim SY, Kim YT, Kim E-A, Lee S-H, Ko S-C, Wijesinghe WAJP, Samarakoon KW, Kim Y-S, Cho JH, Jang H-S, Jeon Y-J (2014) In vitro and in vivo antioxidant activities of polysaccharide purified from aloe vera (Aloe barbadensis) gel. Carbohydr Polym 99:365–371CrossRefGoogle Scholar
  21. Kang Y, Wang Y, Xiong S, Xiong S, Zhang M, Zhu Y (2006) Study on composition and bioactivity of fucoidan from the caudex of Undaria pinnatifida. Chin Pharm J 41:1748–1750Google Scholar
  22. Kim E-A, Lee S-H, Ko C-I, Cha S-H, Kang M-C, Kang S-M, Ko S-C, Lee W-W, Ko J-Y, Lee J-H, Kang N, Oh J-Y, Ahn G, Jee YH, Jeon Y-J (2014) Protective effect of fucoidan against AAPH-induced oxidative stress in zebrafish model. Carbohydr Polym 102:185–191CrossRefGoogle Scholar
  23. Kim KN, Heo SJ, Yoon WJ, Kang SM, Ahn G, Yi TH, Jeon YJ (2010) Fucoxanthin inhibits the inflammatory response by suppressing the activation of NF-κB and MAPKs in lipopolysaccharide-induced RAW 264.7 macrophages. Eur J Pharmacol 649:369–375CrossRefGoogle Scholar
  24. Kraan S (2012) Algal polysaccharides, novel applications and outlook. In: Chang C-F (ed) Carbohydrates – Comprehensive Studies on Glycobiology and Glycotechnology. INTECH Open Access Publisher, pp 399–532Google Scholar
  25. Lee S-H, Ko C-I, Jee Y, Jeong Y, Kim M, Kim J-S, Jeon Y-J (2013) Anti-inflammatory effect of fucoidan extracted from Ecklonia cava in zebrafish model. Carbohydr Polym 92:84–89CrossRefGoogle Scholar
  26. Li B, Lu F, Wei X, Zhao R (2008) Fucoidan: structure and bioactivity. Molecules 13:1671–1695CrossRefGoogle Scholar
  27. Mian AJ, Percival E (1973) Carbohydrates of the brown seaweeds Himanthalia lorea, Bifurcaria bifurcata, and Padina pavonia: Part I extraction and fractionation. Carbohydr Res 26:133–146CrossRefGoogle Scholar
  28. Mizutani S, Deguchi S, Kobayashi E, Nishiyama E, Sagawa H, Kato I (2010) Fucoidan-containing cosmetics. USA patent 7678368Google Scholar
  29. Park E-J, Choi J-i (2017) Melanogenesis inhibitory effect of low molecular weight fucoidan from Undaria pinnatifida. J Appl Phycol 29:2213–2217CrossRefGoogle Scholar
  30. Park HY, Han MH, Park C, Jin C-Y, Kim G-Y, Choi I-W, Kim ND, Nam T-J, Kwon TK, Choi YH (2011) Anti-inflammatory effects of fucoidan through inhibition of NF-κB, MAPK and Akt activation in lipopolysaccharide-induced BV2 microglia cells. Food Chem Toxicol 49:1745–1752CrossRefGoogle Scholar
  31. Park K-J, Park S-H, Kim J-K (2010) Anti-wrinkle activity of Acanthopanax senticosus extract in ultraviolet B (UVB)-induced photoaging. J Korean Soc Food Sci Nutr 39:42–46CrossRefGoogle Scholar
  32. Sanjeewa KKA, Fernando IPS, Samarakoon KW, Lakmal HHC, Kim E-A, Kwon O-N, Dilshara MG, Lee J-B, Jeon Y-J (2016) Anti-inflammatory and anti-cancer activities of sterol rich fraction of cultured marine microalga Nannochloropsis oculata. Algae 31:277–287CrossRefGoogle Scholar
  33. Vo TS, Kim SK (2012) Cosmeceutical compounds from marine sources. In: Kirk-Othmer Enc Chem Technol.
  34. Wang Z-J, Si Y-X, Oh S, Yang J-M, Yin S-J, Park Y-D, Lee J, Qian G-Y (2012) The effect of fucoidan on tyrosinase: computational molecular dynamics integrating inhibition kinetics. J Biomol Struct Dyn 30:460–473CrossRefGoogle Scholar
  35. Wijesinghe WAJP, Jeon Y-J (2011) Biological activities and potential cosmeceutical applications of bioactive components from brown seaweeds: a review. Phytochem Rev 10:431–443CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Marine Life ScienceJeju National UniversityJejuRepublic of Korea
  2. 2.Industrial Technology Institute (ITI)Colombo 7Sri Lanka
  3. 3.Department of Family Medicine College of MedicineDong-A University, Dong-A University Medical CenterBusanSouth Korea
  4. 4.Department of ChemistryUniversity of ColomboColombo 3Sri Lanka

Personalised recommendations