International Ophthalmology

, Volume 39, Issue 12, pp 2757–2766 | Cite as

Effect of intracameral human cord blood-derived stem cells on lasered rabbit trabecular meshwork

  • Ramanjit SihotaEmail author
  • Seema Sen
  • Sujata Mohanty
  • Mohammad Ahmad
  • Alok Ravi
  • Viney Gupta
  • Neerja Bhatla
Original Paper



This study aimed to investigate the effect of intracameral human cord blood stem cells on lasered rabbit trabecular meshwork.


Immediately following diode laser application to the trabecular meshwork, human cord blood stem cells were injected intracamerally, in one eye of 12 albino rabbits. The other eye of ten rabbits was lasered controls and two eyes were normal controls. Rabbits were killed after 4, 8 and 12 weeks.


Lasered control rabbit eyes showed significant disruption of trabecular architecture, loss and pleomorphism of trabecular endothelial cells and progressive narrowing of trabecular spaces till 12 weeks. In contrast, lasered eyes, concurrently injected with human cord blood stem cells, showed relatively preserved endothelial cellularity and structure of the trabecular meshwork, at all time points. Human CD34- and CD44-positive cells were identified in 7/8 eyes treated with stem cells, at 4 and 8 weeks, and 2 of 3 at 12 weeks. Many PKH26-labeled human cord blood cells were visible throughout the trabecular area at 4 weeks. They gradually decreased in number by 8 weeks, and at 12 weeks, they appeared to be oriented along trabecular beams.


There was a relative preservation of cellularity and architecture of the trabecular meshwork in eyes injected with human cord blood stem cells, as compared to lasered control eyes up to 12 weeks, without significant inflammation. This suggests a probable role for such stem cells in eyes with glaucoma, having trabecular dysfunction.


Intracameral cord blood cells Trabecular meshwork Stem cells 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving animals

The study was carried out after clearance from our Institutional Animal Ethics and Stem Cell Ethics Committees. The animals were housed in pathogen-free conditions and treated as per the ARVO statement on the use of animals in ophthalmic and vision research. All applicable international, national and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

Statement on human rights

All procedures performed in studies involving human participants were in accordance with the ethical standards of the 1964 Helsinki declaration and its later amendments.

Informed consent

Informed consent was obtained from all stem cell donors included in the study.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Ramanjit Sihota
    • 1
    Email author
  • Seema Sen
    • 2
  • Sujata Mohanty
    • 3
  • Mohammad Ahmad
    • 2
  • Alok Ravi
    • 1
  • Viney Gupta
    • 1
  • Neerja Bhatla
    • 4
  1. 1.Glaucoma Research Facility and Clinical Services, Dr Rajendra Prasad Centre for Ophthalmic SciencesAll India Institute of Medical SciencesNew DelhiIndia
  2. 2.Ocular PathologyAll India Institute of Medical SciencesNew DelhiIndia
  3. 3.Stem Cell FacilityAll India Institute of Medical SciencesNew DelhiIndia
  4. 4.Obstetrics and GynaecologyAll India Institute of Medical SciencesNew DelhiIndia

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