The involvement of the mGluR5-mediated JNK signaling pathway in rats with diabetic retinopathy
To understand the involvement of the mGluR5-mediated JNK signaling pathway in rats with diabetic retinopathy (DR).
This study established rat models of diabetes mellitus (DM), which were divided into Normal, DM, DM + CHPG (mGluR5 agonist CHPG), and DM + MTEP (mGluR5 antagonist MTEP) groups. The blood glucose and weight of rats were recorded. EB staining was used for observation of blood–retinal barrier (BRB) damage. Neural retina function was measured by pattern electroretinogram (ERG). PAS and NG2 immunohistochemistry were conducted to evaluate the retinal vascular morphology. The TUNEL assay and active caspase-3 immunohistochemistry were performed to detect retinal cell apoptosis. Additionally, the expression levels of superoxide dismutase (SOD) and methylenedioxyamphetamine (MDA) were measured. Moreover, expression levels of mGluR5 and JNK pathway-related proteins were detected by western blot.
When compared with control rats, rats in the DM group showed decreased amplitude and latency of the peak times in the ERG test; further, DM group rats presented increases in blood glucose, BRB permeability, a retinal capillary area density, retinal cell apoptosis with an increased number of active caspase-3-positive cells, MDA level, mGluR5 levels, and the ratio of p-JNK/JNK, and they showed reductions in body weight and SOD activity, as well as in the number of pericytes and in the pericyte coverage (all P < 0.05). However, rats in DM + CHPG group had stronger negative effects than those in DM group (all P < 0.05). Rats from DM + MTEP group showed an opposite trend compared with the DM rats (all P < 0.05).
The level of mGluR5 in DR rats was upregulated, whereas inhibition of mGluR5 alleviated retinal pathological damage and decreased cell apoptosis to improve DR via suppression of the JNK signaling pathway, which provided a scientific theoretical basis for the clinical treatment of DR.
KeywordsmGluR5 JNK signaling pathway Diabetes mellitus Diabetic retinopathy Apoptosis
The authors appreciate the reviewers for their useful comments in this paper.
Compliance with ethical standards
Conflict of interest
No potential conflicts of interest were disclosed.
The animal experimental design used in this study was approved by the Experimental Animal Ethics Committee of the First People’s Hospital of Jingzhou; all experimental animal behaviors strictly followed the laboratory animal management and operation guide issued by the National Institutes of Health.
- 14.Bayne K (1996) Revised guide for the care and use of laboratory animals available. American Physiological Society. Physiologist 39(199):111–208Google Scholar
- 15.Zheng Z, Chen H, Ke G, Fan Y, Zou H et al (2009) Protective effect of perindopril on diabetic retinopathy is associated with decreased vascular endothelial growth factor-to-pigment epithelium-derived factor ratio: involvement of a mitochondria-reactive oxygen species pathway. Diabetes 58:954–964CrossRefGoogle Scholar
- 18.Chen W, Yao X, Zhou C, Zhang Z, Gui G et al (2017) Danhong huayu koufuye prevents diabetic retinopathy in streptozotocin-induced diabetic rats via antioxidation and anti-inflammation. Mediat Inflamm 2017:3059763Google Scholar
- 42.Huang XP, Qiu YY, Wang B, Ding H, Tang YH et al (2014) Effects of Astragaloside IV combined with the active components of Panax notoginseng on oxidative stress injury and nuclear factor-erythroid 2-related factor 2/heme oxygenase-1 signaling pathway after cerebral ischemia-reperfusion in mice. Pharmacogn Mag 10:402–409CrossRefGoogle Scholar