International Ophthalmology

, Volume 38, Issue 5, pp 2133–2140 | Cite as

A comparative study: deep anterior lamellar keratoplasty for corneal dystrophies using two different donor stromal tissues

  • Yusuf KoçlukEmail author
  • Emine Alyamaç Sukgen
Original Paper



This study aimed to present a comparison of early postoperative results of deep anterior lamellar keratoplasty (DALK) surgery performed using the two different corneal graft sources.

Materials and methods

This retrospective clinical study included the surgery performed on 26 eyes of 26 patients who underwent DALK for the stromal dystrophies. At the time of surgery, donor stromal tissue for DALK was sourced using the two different preparatory techniques. For 14 cases (Group 1), the donor grafts were prepared quickly by removing the descemet’s membrane (DM) without much care after the exposure of the bare DM in the recipient cornea. For 12 cases (Group 2), the grafts for DALK surgery had already been prepared during a previous descemet’s membrane endothelial keratoplasty (DMEK).


The groups differed statistically regarding the blue staining of the grafts at the time of surgery and on the first day post-DALK. The first day after surgery, 14 (100%) grafts were clear in Group 1, while eight (66.7%) grafts were clear and four (33.3%) were edematous in Group 2 (p = 0.019). Bubbling was performed in three (25.0% of Group 2) of the four cases on the first day post-DALK, while none of the patients in Group 1 underwent this procedure (p = 0.47). The postoperative increase in visual acuity was higher in Group 1 in the early postoperative period (p = 0.012).


During the early postoperative period, complications (as graft edema, detachment, re-bubbling) were observed more frequently in the DALK operations performed with stromal tissue having blue stain remaining from the DMEK performed on the same day and in the same session.


Deep anterior lamellar keratoplasty Descemet’s membrane endothelial keratoplasty Split keratoplasty Trypan blue solution Stromal dystrophies 



All authors certify that they have no affiliations with or involvement in any organization or entity with any financial interest (such as honoraria; educational grants; participation in speakers’ bureaus; membership, employment, consultancies, stock ownership or other equity interest; and expert testimony or patent-licensing arrangements) or non-financial interest (such as personal or professional relationships, affiliations, knowledge or beliefs) in the subject matter or materials discussed in this manuscript.

Compliance with ethical standards

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Conflicts of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.


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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  1. 1.Department of OphthalmologyAdana Numune Training and Research HospitalYüreğir, AdanaTurkey

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