Skip to main content

Advertisement

SpringerLink
Log in

Endothelin-1 levels and biomarkers of oxidative stress in glaucoma patients

  • Original Paper
  • Published:
International Ophthalmology Aims and scope Submit manuscript

Abstract

To investigate the levels of endothelin-1 (ET-1), homocysteine (Hcy), vitamins A, E, B12 and folic acid in plasma of patients with different types of glaucoma: primary open-angle glaucoma (POAG) and normotensive glaucoma (NTG). Patients were classified into 3 groups: group POAG comprised 48 patients, group NTG comprised 15 patients, and control group that comprised 75 healthy subjects. ET-1 levels were measured by ELISA, Vitamins A and E by HPLC, and Vitamin B12, homocysteine, and folic acid levels were determined by chemiluminescent immunoassay. The ET-1 and Hcy levels were significantly higher (p = 0.002) in the POAG group compared to NTG and control group. Vitamin E levels were significantly lower (p = 0.001) in the NTG group compared to POAG and control group. The increase of Hcy and ET-1 in POAG patients is related to vascular endothelial dysfunction. Thus results may play a key role in the development of this disease. Lower levels of Vitamin E in the NTG group suggest that oxidative process plays an early role in the development of this type of glaucoma.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Yanagi M, Kawasaki R, Wang JJ, Wong TY, Crowston J, Kiuchi Y (2011) Vascular risk factors in glaucoma: a review. Clin Exp Ophthalmol 39(3):252–258. doi:10.1111/j.1442-9071.2010.02455.x

    Article  Google Scholar 

  2. Shields MB (2008) Normal-tension glaucoma: is it different from primary open-angle glaucoma? Curr Opin Ophthalmol 19(2):85–88

    Article  PubMed  Google Scholar 

  3. Flammer J, Konieczka K, Flammer AJ (2013) The primary vascular dysregulation syndrome: implications for eye diseases. EPMA J 4(1):14. doi:10.1186/1878-5085-4-14

    Article  PubMed Central  PubMed  Google Scholar 

  4. Mroczkowska S, Benavente-Perez A, Negi A, Sung V, Patel SR, Gherghel D (2013) Primary open-angle glaucoma vs normal-tension glaucoma: the vascular perspective. JAMA Ophthalmol 131(1):36–43. doi:10.1001/2013.jamaophthalmol.1

    Article  PubMed  Google Scholar 

  5. Erb C, Heinke M (2011) Oxidative stress in primary open-angle glaucoma. Front Biosci 3:1524–1533

    Google Scholar 

  6. Sugiyama T, Moriya S, Oku H, Azuma I (1995) Association of endothelin-1 with normal tension glaucoma: clinical and fundamental studies. Surv Ophthalmol 39(Suppl 1):S49–S56

    Article  PubMed  Google Scholar 

  7. Kaiser HJ, Flammer J, Wenk M, Luscher T (1995) Endothelin-1 plasma levels in normal-tension glaucoma: abnormal response to postural changes. Graefes Arch Clin Exp Ophthalmol 233(8):484–488

    Article  CAS  PubMed  Google Scholar 

  8. Emre M, Orgul S, Haufschild T, Shaw SG, Flammer J (2005) Increased plasma endothelin-1 levels in patients with progressive open angle glaucoma. Br J Ophthalmol 89(1):60–63

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  9. Good TJ, Kahook MY (2010) The role of endothelin in the pathophysiology of glaucoma. Expert Opin Ther Targets 14(6):647–654

    Article  CAS  PubMed  Google Scholar 

  10. Cellini M, Strobbe E, Gizzi C, Balducci N, Toschi PG, Campos EC (2012) Endothelin-1 plasma levels and vascular endothelial dysfunction in primary open angle glaucoma. Life sci 13-14:699–702

    Article  Google Scholar 

  11. Chauhan BC (2008) Endothelin and its potential role in glaucoma. Can J Ophthalmol 43(3):356–360

    Article  PubMed  Google Scholar 

  12. Chen HY, Chang YC, Chen WC, Lane HY (2013) Association between plasma endothelin-1 and severity of different types of glaucoma. J Glaucoma 22(2):117–122

    Article  PubMed  Google Scholar 

  13. Mozaffarieh M, Grieshaber MC, Orgül S, Flammer J (2008) The potential value of natural antioxidative treatment in glaucoma. Surv Ophthalmol 53(5):479–505

    Article  CAS  PubMed  Google Scholar 

  14. Bleich S, Kornhuber J, Junemann AG (2003) Homocysteine in primary and secondary open-angle glaucoma. J Glaucoma 12(6):498–499

    Article  PubMed  Google Scholar 

  15. Mujumdar VS, Aru GM, Tyagi SC (2001) Induction of oxidative stress by homocysteine impairs endothelial function. J Cell Biochem 82(3):491–500

    Article  CAS  PubMed  Google Scholar 

  16. Tyagi N, Sedoris KC, Steed M, Ovechkin AV, Moshal KS, Tyagi SC (2005) Mechanisms of homocysteine-induced oxidative stress. Am J Physiol Heart Circ Physiol 289(6):H2649–H2656

    Article  CAS  PubMed  Google Scholar 

  17. Wang G, Medeiros FA, Barshop BA, Weinreb RN (2004) Total plasma homocysteine and primary open-angle glaucoma. Am J Ophthalmol 137(3):401–406

    Article  CAS  PubMed  Google Scholar 

  18. Cumurcu T, Sahin S, Aydin E (2006) Serum homocysteine, vitamin B12 and folic acid levels in different types of glaucoma. BMC Ophthalmol 6:6

    Article  PubMed Central  PubMed  Google Scholar 

  19. Clement CI, Goldberg I, Healey PR, Graham SL (2009) Plasma homocysteine, MTHFR gene mutation, and open-angle glaucoma. J Glaucoma 18(1):73–78

    Article  PubMed  Google Scholar 

  20. Turgut B, Kaya M, Arslan S, Demir T, Guler M, Kaya MK (2010) Levels of circulating homocysteine, vitamin B6, vitamin B12, and folate in different types of open-angle glaucoma. Clin Interv Aging 5:33–39

    Google Scholar 

  21. Rossler CW, Baleanu D, Reulbach U et al (2010) Plasma homocysteine levels in patients with normal tension glaucoma. J Glaucoma 19(9):576–580

    Article  PubMed  Google Scholar 

  22. Tezel G (2006) Oxidative stress in glaucomatous neurodegeneration: mechanisms and consequences. Prog Retin Eye Res 25(5):490–513

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  23. Izzotti A, Bagnis A, Sacca SC (2006) The role of oxidative stress in glaucoma. Mutat Res 612(2):105–114

    Article  CAS  PubMed  Google Scholar 

  24. Zanon-Moreno V, Marco-Ventura P, Lleo-Perez A et al (2008) Oxidative stress in primary open-angle glaucoma. J Glaucoma 17(4):263–268

    Article  PubMed  Google Scholar 

  25. Yuki K, Murat D, Kimura I, Tsubota K (2010) Increased serum total antioxidant status and decreased urinary 8-hydroxy-2′-deoxyguanosine levels in patients with normal-tension glaucoma. Acta Ophthalmol 88(7):259–264

    Article  Google Scholar 

  26. Engin K, Yemisci B, Yigit U, Agachan A, Coskun C (2010) Variability of serum oxidative stress biomarkers relative to biochemical data and clinical parameters of glaucoma patients. Mol Vision 16:1260–1271

    CAS  Google Scholar 

  27. He Y, Leung KW, Zhang YH et al (2008) Mitochondrial complex I defect induces ROS release and degeneration in trabecular meshwork cells of POAG patients: protection by antioxidants. Invest Ophthalmol Vis Sci 49(4):1447–1458

    Article  PubMed  Google Scholar 

  28. Sacca SC, Izzotti A (2008) Oxidative stress and glaucoma: injury in the anterior segment of the eye. Prog Brain Res 173:385–407

    Article  CAS  PubMed  Google Scholar 

  29. Harada T, Harada C, Nakamura K et al (2007) The potential role of glutamate transporters in the pathogenesis of normal tension glaucoma. J Clin Invest 117(7):1763–1770

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  30. Galassi F, Giambene B, Varriale R (2011) Systemic vascular dysregulation and retrobulbar hemodynamics in normal-tension glaucoma. Invest Ophthalmol Vis Sci 52(7):4467–4471. doi:10.1167/iovs.10-6710

    Article  PubMed  Google Scholar 

  31. Kang JH, Pasquale LR, Willett W et al (2003) Antioxidant Intake and Primary Open-Angle Glaucoma: a Prospective Study. Am J Epidemiol 158:337–346

    Article  PubMed  Google Scholar 

  32. Mares JA (2004) High-dose antioxidant supplementation and cataract risk. Nutr Rev 62(1):28–32

    Article  PubMed  Google Scholar 

  33. Evans J (2008) Antioxidant supplements to prevent or slow down the progression of AMD: a systematic review and meta-analysis. Eye 22(6):751–760

    Article  CAS  PubMed  Google Scholar 

  34. Ko M, Peng P, Hsu S, Chen Ch (2010) Dietary deficiency of vitamin E aggravates retinal ganglion cell death in experimental glaucoma of rats. Curr Eye Res 35(9):842–849

    Article  CAS  PubMed  Google Scholar 

  35. Ghanem AA, Elewa AM, Arafa LF (2011) Endothelin-1 and nitric oxide levels in patients with glaucoma. Ophalmic Res 46(2):98–102

    Article  CAS  Google Scholar 

  36. Tousoulis D, Bouras G, Antoniades C et al (2010) The activation of endothelin-1 pathway during methionine-induced homocysteinemia mediates endotelial dysfunction in hypertensive individuals. J Hypertens 28(5):925–930

    Article  CAS  PubMed  Google Scholar 

  37. Lee DJ, Gomez-Marin O, Lam BL, Zheng DD (2003) Glaucoma and survival: the National Health Interview Survey 1986-1994. Ophthalmology 110(8):1476–1483

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

This study received financial support from the University Hospital of Elche Foundation (Spain) (FIBElx08/02 and FIBElx09/11).

Conflict of interest

The author(s) declare that they have no conflict of interest.

Author information

Authors and Affiliations

  1. Clinical Laboratory, General University Hospital, Elche, Spain

    N. López-Riquelme, C. Villalba, C. Tormo & A. Belmonte

  2. Ophthalmology Department, General University Hospital, Elche, Spain

    C. Fernandez, G. Torralba & F. Hernández

  3. Department of Biochemistry, University Hospital of Elche Camino de La Almazara, 11 03203, Elche, Alicante, Spain

    N. López-Riquelme

Authors
  1. N. López-Riquelme
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. Villalba
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. Tormo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. Belmonte
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. C. Fernandez
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. G. Torralba
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. F. Hernández
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to N. López-Riquelme.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

López-Riquelme, N., Villalba, C., Tormo, C. et al. Endothelin-1 levels and biomarkers of oxidative stress in glaucoma patients. Int Ophthalmol 35, 527–532 (2015). https://doi.org/10.1007/s10792-014-9979-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10792-014-9979-8

Keywords

Search

Navigation