, Volume 27, Issue 6, pp 1155–1167 | Cite as

Antinociceptive effect of flavonol and a few structurally related dimethoxy flavonols in mice

  • Vijaykumar SayeliEmail author
  • Jagan Nadipelly
  • Parimala Kadhirvelu
  • Binoy Varghese Cheriyan
  • Jaikumar Shanmugasundaram
  • Viswanathan Subramanian
Original Article


Previous reports suggest flavonoids as potent analgesic compounds. Based on these observations, the present study investigated the antinociceptive action of flavonol, 3′, 4′-dimethoxy flavonol, 6, 3′-dimethoxy flavonol, 7, 2′-dimethoxy flavonol, and 7, 3′-dimethoxy flavonol and the possible mechanisms involved in these effects. The antinociceptive effect of the investigated compounds in doses of 25, 50, 100, and 200 mg/kg was evaluated in male Swiss albino mice using the acetic acid test, formalin-induced nociception, and hot water tail immersion test. The role of opioid, tryptaminergic, adrenergic, dopaminergic, GABAergic, and K+ATP channels in producing the antinociceptive effect was also studied using appropriate interacting agents. Treatment with flavonol and dimethoxy flavonols resulted in a significant reduction in the number of abdominal constrictions in the acetic acid test, a significant inhibition of the paw-licking/biting response time in both the phases of formalin nociception and also a significant increase in mean reaction time in the hot water tail immersion test. These observations revealed the antinociceptive effect of dimethoxy flavonols. The role of opioid, serotonergic (5HT3), and dopaminergic system was identified in the antinociceptive effect of flavonol and all dimethoxy derivatives investigated. In addition, the role of GABAergic, K+ATP channel, and α-2 adrenergic mechanisms were also observed in the antinociceptive action of some of the investigated compounds. The present study identified the antinociceptive effect of flavonol and dimethoxy flavonols in mice acting through different neuronal pathways.


Flavonol derivatives Antinociception Neuronal mechanisms Opioid Serotonergic Dopaminergic GABAergic Adrenergic 



The support extended by Meenakshi Academy of Higher education & Research (Deemed to be University) for the study is gratefully acknowledged.

Author contributions

VSA and VSU designed the work. VSU, JN, BVC, and PK carried out the experiments, collected, and analyzed the data. VSA, VSU, and JS drafted the manuscript and revised it critically. All authors agree to be accountable for all aspects of the work.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Statement of welfare on animals

All procedures performed in studies involving animals were in accordance with the ethical standards of the institution following the guidelines of the Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA) Government of India.


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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Department of PharmacologyMamata Medical CollegeKhammamIndia
  2. 2.Department of Pharmacology, Faculty of MedicineTexila American UniversityGeorgetownGuyana
  3. 3.Department of PharmacologyMeenakshi Medical College and Research Institute, Meenakshi Academy of Higher Education and Research (Deemed To Be University)KanchipuramIndia
  4. 4.Department of Pharmaceutical ChemistryVISTAS, VELS School of Pharmaceutical SciencesChennaiIndia

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