Curcumin ameliorates palmitate-induced inflammation in skeletal muscle cells by regulating JNK/NF-kB pathway and ROS production
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Curcumin, a natural polyphenol compound, has the beneficial effects on several diseases such as metabolic syndrome, cancer, and diabetes. The anti-inflammatory property of curcumin has been demonstrated in different cells; however, its role in prevention of palmitate-induced inflammation in skeletal muscle C2C12 cells is not known. In this study, we examined the effect of curcumin on the inflammatory responses stimulated by palmitate in C2C2 cells. The results showed that palmitate upregulated the mRNA expression and protein release of IL-6 and TNF-α cytokines in C2C12 cells, while pretreatment with curcumin was able to attenuate the effect of palmitate on inflammatory cytokines. The anti-inflammatory effect of curcumin was associated with the repression of phosphorylation of IKKα-IKKβ, and JNK. Palmitate also caused an increase in reactive oxygen species (ROS) level that curcumin abrogated it. Collectively, these findings suggest that curcumin may represent a promising therapy for prevention of inflammation in skeletal muscle cells.
KeywordsCurcumin Inflammation Skeletal muscle cells NF-kB JNK TNF-α IL-6 Reactive oxygen species
Free fatty acids
Tumor necrosis factor alpha
Nuclear factor kappa-light-chain-enhancer of activated B cells
Mitogen-activated protein kinase
c-Jun N-terminal kinase
Extracellular signal-regulated kinase
Reactive oxygen species
Type 2 diabetes
Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor
This work was financially supported by a Grant (96-04-30-36707) from the Deputy of Research, Tehran University of Medical Sciences.
Compliance with ethical standards
Conflict of interest
The authors have nothing to declare.
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