Biological Effects of 25 to 150 GHz Radiation After In Vitro Exposure of Human Fibroblasts: a Comparison of Experimental Results
In this paper, we present a comprehensive discussion of the results obtained after in vitro exposure of human fetal fibroblasts and human adult fibroblasts to pulsed radiation in a wide band between 100 and 150 GHz and to continuous wave radiation at 25 GHz. In order to assess potential effects of exposure, the genome integrity, cell cycle, cytological ultrastructure, and proteins expression were evaluated.
KeywordsMillimeter waves Terahertz Biological effects Non-ionizing radiation In vitro exposure Human primary cells Fibroblasts
We gratefully acknowledge the technical support of M. Aquilini, E. Campana, S. Di Giovenale, A. Fastelli, P. Petrolini, and B. Raspante in the design and realization of the exposure setup as well as their skillful assistance during the irradiation experiments.
This work was supported by the Italian Ministry of Defence, SEGREDIFESA/DNA – 5° Department of Technological Innovation (GREAM project).
- 1.M.-O. Mattsson, O. Zeni, M. Simko, Is there a biological basis for therapeutic applications of millimetre waves and THz waves?, J Infrared Milli Terahz Waves (this issue) Google Scholar
- 3.A. Ramundo Orlando, G.P. Gallerano, Terahertz Radiation Effects and Biological Applications, J Infrared Milli Terahz Waves 30, 1308–1318 (2009)Google Scholar
- 6.G.P. Gallerano; E. Grosse, R. Korenstein, M. Dressel, W. Mantele, M.R. Scarfi, A.C. Cefalas, P. Taday, R.H. Clothier, P. Jepsen, THz-BRIDGE: a European project for the study of the interaction of terahertz radiation with biological systems, Proc. of the Joint 29th International Conference on Infrared and Millimeter Waves and 12th International Conference on Terahertz Electronics Page(s): 817–818 (2004); https://doi.org/10.1109/ICIMW.2004.1422345
- 8.O. Zeni, G.P. Gallerano, A. Perrotta, M. Romanò, A. Sannino, M. Sarti, M. D’Arienzo, A. Doria, E. Giovenale, A. Lai, G. Messina and M.R. Scarfì, Cytogenetic Observations in human peripheral blood leukocytes following in vitro exposure to THz radiation: A pilot study Health Phys. 92(4) 349–357 (2007)CrossRefGoogle Scholar
- 12.Wilmink, G.J., et al. Determination of death thresholds and identification of terahertz (THz)-specific gene expression signatures. in Optical Interactions with Tissues and Cells XXI. 2010: SPIE. 7562: pp.75620K–75620K-8Google Scholar
- 13.A. De Amicis, S. De Sanctis, S. Di Cristofaro, V. Franchini, F. Lista, E. Regalbuto, E. Giovenale, G.P. Gallerano, P. Nenzi, R. Bei, M. Fantini, M. Benvenuto, L. Masuelli, E. Coluzzi, C. Cicia, A. Sgura, Biological effects of in vitro THz radiation exposure in human foetal fibroblasts, Mutation Research (2015) 793: 150–160CrossRefGoogle Scholar
- 14.V. Franchini, S. De Sanctis, J. Marinaccio, A. De Amicis, E. Coluzzi, S. Di Cristofaro, F. Lista, E. Regalbuto, A. Doria, E. Giovenale, G.P. Gallerano, R. Bei, M. Benvenuto, L. Masuelli, I. Udroiu, A. Sgura, Effect of 0.15 THz radiation on genome integrity of adult fibroblasts, Environmental and Molecular Mutagenesis, 2018 Mar 30 https://doi.org/10.1002/em.22192
- 15.V. Franchini, E. Regalbuto, A. De Amicis, S. De Sanctis, S. Di Cristofaro, E. Coluzzi, J. Marinaccio, A. Sgura, S. Ceccuzzi, A. Doria, G.P. Gallerano, E. Giovenale, G.L. Ravera, R. Bei, M. Benvenuto, A. Modesti, L. Masuelli, F. Lista, Genotoxic Effects In Human Fibroblasts Exposed To Microwave Radiation, Health Physics. Health Physics: July 2018 - Volume 115 - Issue 1 - p 126–139, https://doi.org/10.1097/HP.0000000000000871
- 17.M. Lippmann, B.S. Cohen, R.B. Schlesinger, Environmental Health Science: Recognition, Evaluation and Control of Chemical and Physical Health Hazards Oxford University Press (2003)Google Scholar
- 18.T. Kleine-Ostman et al., Field Exposure and Dosimetry in the THz Frequency Range, IEEE Trans-TST, 4, pp.12–25 (2014)Google Scholar
- 22.A. Sgura and D. Cimini, Telomeres and chromosomes segregation in Telomeres: Function, Shortening and Lengthening. Editor: Leonardo Mancini Nova Science Publishers, Inc. (2009)Google Scholar
- 25.D. Remondini, R. Nylund, J. Reivinen, F. Poulletier de Gannes, B. Veyret, I. Lagroye, E. Haro, M.A. Trillo, M. Capri, C. Franceschi, K. Schlatterer, R. Gminski, R. Fitzner, R. Tauber, J. Schuderer, N. Kuster, D. Leszczynski, F. Bersani, C. Maercker, Gene expression changes in human cells after exposure to mobile phone microwaves., Proteomics. 2006 Sep; 6(17):4745–54.CrossRefGoogle Scholar