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Exogenous Hydrogen Sulfide Regulates Mycoplasma pneumoniae Lipid-Associated Membrane Proteins to Induce Expression of Heme Oxygenase-1 and Proinflammatory Cytokines

  • Xinnian Hu
  • Wuwei Zeng
  • Xiaoxing You
  • Weiyan Ding
  • Peng Liu
  • Liesong Chen
  • Yanhua Zeng
  • Cuiming ZhuEmail author
Original Article


This study was designed to investigate the effect of exogenous hydrogen sulfide (H2S) on the secretion of Heme oxygenase (HO-1) and proinflammatory cytokines in human mononuclear cell line THP-1 stimulated by lipid-associated membrane proteins (LAMPs) prepared from Mycoplasma pneumoniae (M. pneumoniae) and explore its regulatory mechanism. Cultured cells were stimulated with M. pneumoniae LAMPs after pretreatment with H2S to analyze the production of proinflammatory cytokines and HO-1 by enzyme-linked immunosorbent assay (ELISA) and Western blot. The results showed that THP-1 cells, which were stimulated by LAMPs after pretreatment with H2S, had decreased production of interleukin-6 (IL-6) and interleukin-8 (IL-8) by inhibiting the mitogen-activated protein kinases (MAPKs)/nuclear factor-kappa B (NF-κB) signaling pathway and increased expression of HO-1 by activating the nuclear factor E2–related factor 2 (Nrf2) signaling pathway. Our results indicate that H2S may play an important role in attenuating inflammation induced by M. pneumoniae LAMPs due to its ability to decrease the production of IL-6 and IL-8 and increase the expression of the HO-1. These findings support further studies for possible clinical applications.


mycoplasma pneumoniae hydrogen sulfide (H2S) lipid-associated membrane proteins (LAMPs) proinflammatory cytokines Heme oxygenase-1(HO-1) 



Hydrogen sulfide


Heme oxygenase-1


Human monocytic cell line


Lipid associated membrane proteins


Mitogen-activated protein kinase


Nuclear factor-kappa B


Community-acquired bacterial pneumonias


Toll-like receptors




Myeloid differentiation factor 88


Interleukin-1 receptor–associated kinase


Tumor necrosis factor receptor–associated factor 6


Activating protein 1








Tumor necrosis factor


Reactive oxygen species


Nuclear factor E2–related factor 2




Inducible nitric oxide synthase


Nitric oxide


Prostaglandin E2


Carbon monoxide


Roswell Park Memorial Institute


Fetal bovine serum


Tris-buffered saline


Phosphate-buffered saline


Polyvinylidene difluoride


Tris-buffered saline with Tween



We thank Xiaoxing You for providing the exogenous hydrogen sulfide. We also thank Peng Liu for modifying the manuscript.

Authors’ Contributions

All authors have approved this manuscript

Funding Information

This work was supported by the National Natural Science Foundation of China (No. 81441065, No. 31970177); Natural Science Foundation of Hunan Province, China (Grant No. 2019JJ40253); Key Fund Project of Hunan Provincial Department of Education (Grant No. 18A234); and Scientific Research Foundation for the Returned Overseas Chinese Scholars, University of South China (Grant No. 2017XQD25).

Compliance with Ethical Standards

Competing Interests

The authors declare that they have no competing interests.

Ethics Approval

Study procedures were approved by the University of South China.

Supplementary material

10753_2019_1170_MOESM1_ESM.docx (39 kb)
ESM 1 (DOCX 39 kb)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Institute of Pathogenic Biology, Hengyang Medical College, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hunan Province Cooperative Innovation Center for Molecular Target New Drug StudyUniversity of South ChinaHengyangChina
  2. 2.Qingyuan Maternal and Child Health HospitalQingyuanChina

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