Exogenous Hydrogen Sulfide Regulates Mycoplasma pneumoniae Lipid-Associated Membrane Proteins to Induce Expression of Heme Oxygenase-1 and Proinflammatory Cytokines
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This study was designed to investigate the effect of exogenous hydrogen sulfide (H2S) on the secretion of Heme oxygenase (HO-1) and proinflammatory cytokines in human mononuclear cell line THP-1 stimulated by lipid-associated membrane proteins (LAMPs) prepared from Mycoplasma pneumoniae (M. pneumoniae) and explore its regulatory mechanism. Cultured cells were stimulated with M. pneumoniae LAMPs after pretreatment with H2S to analyze the production of proinflammatory cytokines and HO-1 by enzyme-linked immunosorbent assay (ELISA) and Western blot. The results showed that THP-1 cells, which were stimulated by LAMPs after pretreatment with H2S, had decreased production of interleukin-6 (IL-6) and interleukin-8 (IL-8) by inhibiting the mitogen-activated protein kinases (MAPKs)/nuclear factor-kappa B (NF-κB) signaling pathway and increased expression of HO-1 by activating the nuclear factor E2–related factor 2 (Nrf2) signaling pathway. Our results indicate that H2S may play an important role in attenuating inflammation induced by M. pneumoniae LAMPs due to its ability to decrease the production of IL-6 and IL-8 and increase the expression of the HO-1. These findings support further studies for possible clinical applications.
KEY WORDSmycoplasma pneumoniae hydrogen sulfide (H2S) lipid-associated membrane proteins (LAMPs) proinflammatory cytokines Heme oxygenase-1(HO-1)
Human monocytic cell line
Lipid associated membrane proteins
Mitogen-activated protein kinase
Nuclear factor-kappa B
Community-acquired bacterial pneumonias
Myeloid differentiation factor 88
Interleukin-1 receptor–associated kinase
Tumor necrosis factor receptor–associated factor 6
Activating protein 1
Tumor necrosis factor
Reactive oxygen species
Nuclear factor E2–related factor 2
Inducible nitric oxide synthase
Roswell Park Memorial Institute
Fetal bovine serum
Tris-buffered saline with Tween
We thank Xiaoxing You for providing the exogenous hydrogen sulfide. We also thank Peng Liu for modifying the manuscript.
All authors have approved this manuscript
This work was supported by the National Natural Science Foundation of China (No. 81441065, No. 31970177); Natural Science Foundation of Hunan Province, China (Grant No. 2019JJ40253); Key Fund Project of Hunan Provincial Department of Education (Grant No. 18A234); and Scientific Research Foundation for the Returned Overseas Chinese Scholars, University of South China (Grant No. 2017XQD25).
Compliance with Ethical Standards
The authors declare that they have no competing interests.
Study procedures were approved by the University of South China.
- 7.Hu, J., C. Chen, G. Ou, X. You, T. Tan, X. Hu, et al. 2017. Nrf2 regulates the inflammatory response, including heme oxygenase-1 induction, by Mycoplasma pneumoniae lipid associated membrane proteins in THP-1 cells. Pathogens & Disease.Google Scholar
- 18.Wu, Y., D. Li, Y. Wang, X. Liu, Y. Zhang, W. Qu, et al. 2018. Beta-defensin 2 and 3 promote bacterial clearance of Pseudomonas aeruginosa by inhibiting macrophage autophagy through downregulation of early growth response gene-1 and c-FOS. Frontiers in Immunology 9.Google Scholar