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Novel Treatment of Experimental Autoimmune Prostatitis by Nanoparticle-Conjugated Autoantigen Peptide T2

  • Yijie Cheng
  • Yanfang Cao
  • Awais Ullah Ihsan
  • Farhan Ullah Khan
  • Xue Li
  • Dianyou Xie
  • Xingxing Cui
  • Wenlu Wang
  • Ziwei Liu
  • Cunyu Li
  • Khalil Ali Ahmad
  • Kiganda Raymond Sembatya
  • Reyaj Mikrani
  • Xiaohui ZhouEmail author


The exact etiology and pathogenesis of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) are still unknown, as a result, available therapeutic options for patients are far from satisfactory. Therefore, there is a need to develop a valid therapeutic approach that can ameliorate the manifestations of CP/CPPS. Fifty male C57BL/6 mice were randomly divided into five groups of ten mice each. All groups except naïve were subcutaneously injected with 0.2 ml of T2 plus complete Freund adjuvant (CFA) on day 0 and 14 to generate valid CP/CPPS model. After successful CP/CPPS induction, model group was injected with 0.2 ml of normal saline while PLGA, PLGA-OVA, and PLGA-T2 groups were administered intravenously with 0.2 ml mixture of PLGA, PLGA-OVA, and PLGA-T2, respectively. Voiding behavior, pain threshold, and hematoxylin and eosin staining were used to assess micturition habits, pain intensity as well as prostate inflammation. Additionally, TNF-α, CRP, and IL-10 levels in plasma were measured by using ELISA kits. Mice administered with PLGA-T2 showed higher pain threshold, lower urine frequencies, mild edema, and inflammation in prostate tissue in comparison to other groups. Moreover, the expression of TNF-α and CRP levels was markedly decreased while IL-10 expression was increased in the PLGA-T2 treatment group as compared to the other groups. Our results showed that nanoparticles conjugated with autoantigen novel peptide T2 could successfully alleviate or even heal CP/CPPS to some extent in mice. This study provides an easy, useful, and economic tool for ameliorating the manifestations of CP/CPPS that will improve the therapeutic approaches.

Key Words

Chronic prostatitis Autoimmune disease Nanoparticle T2 peptide 



This work was supported by the National Natural Science Foundation of China (grant numbers 30973003; 30901993); and the Administration of TCM of Jiangsu Province (grant number LZ11093).

Compliance with Ethical Standards

Conflict of Interest

All authors declare no conflict of interest.

Submission Declaration and Verification

This manuscript has not been published previously, and it is not under consideration elsewhere. All authors approved to submit this manuscript to this journal.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Yijie Cheng
    • 1
  • Yanfang Cao
    • 1
  • Awais Ullah Ihsan
    • 1
  • Farhan Ullah Khan
    • 1
    • 2
  • Xue Li
    • 1
  • Dianyou Xie
    • 1
  • Xingxing Cui
    • 1
  • Wenlu Wang
    • 1
  • Ziwei Liu
    • 1
  • Cunyu Li
    • 1
  • Khalil Ali Ahmad
    • 2
  • Kiganda Raymond Sembatya
    • 1
  • Reyaj Mikrani
    • 1
  • Xiaohui Zhou
    • 1
    • 3
    • 4
    Email author
  1. 1.Department of Clinical Pharmacy, School of Basic Medicine and Clinical PharmacyChina Pharmaceutical UniversityNanjingChina
  2. 2.School of PharmacyShanghai Jiao Tong UniversityShanghaiChina
  3. 3.Department of SurgeryNanjing Shuiximen HospitalNanjingChina
  4. 4.Department of SurgeryZhongda Hospital Affiliated to Southeast UniversityNanjingPeople’s Republic of China

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