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Schisandrin B Attenuates Inflammation in LPS-Induced Sepsis Through miR-17-5p Downregulating TLR4

  • Zhi-Rong Ji
  • Wei-Liang Xue
  • Ling Zhang
ORIGINAL ARTICLE
  • 12 Downloads

Abstract

To investigate the mechanism of Schisandrin B (Sch B) on the inflammation in LPS-induced sepsis. Sepsis mouse model was established by injecting LPS. qRT-PCR and western blot were used to measure the expression of miR-17-5p and TLR4. ELISA was used to test the concentrations of IL-1β and TNF-α. Sch B could increase miR-17-5p expression, promote inflammation, and decrease TLR4 expression in sepsis mice and LPS-induced macrophages. Moreover, miR-17-5p could negatively regulate TLR4. Overexpression of miR-17-5p suppressed the concentrations of inflammatory factors (IL-1β and TNF-α) in LPS induced-macrophages, while pcDNA-TLR4 could change the inhibition effect. Additionally, miR-17-5p inhibitor changed the inhibitory effects of Sch B on TLR4 expression and the concentrations of IL-1β and TNF-α in LPS induced-macrophages. Sch B could attenuate inflammation in LPS-induced sepsis through miR-17-5p downregulating TLR4.

KEY WORDS

sepsis Schisandrin B miR-17-5p TLR4 inflammation 

Notes

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Traditional Chinese MedicinePeople’s Hospital of Ningxia Hui Autonomous Region, the First Affiliated Hospital of Northwest University for NationalitiesYinchuanChina
  2. 2.Department of EmergencyPeople’s Hospital of Ningxia Hui Autonomous Region, the First Affiliated Hospital of Northwest University for NationalitiesYinchuanChina

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