, Volume 42, Issue 2, pp 714–720 | Cite as

Locally Used Antibiotics for Spinal Infection Prophylaxis and Their Effects on Epidural Fibrosis: an Experimental Laminectomy Study in Rats Using Rifamycin and Gentamycin

  • Nese KeserEmail author
  • Merih Is
  • Duygu Ceman
  • Adnan Somay


The study aims to assess the effects of antibiotics (ABs), which are typically used in spinal infection prophylaxis, on the formation of epidural fibrosis (EF). Specifically, we investigated the effect of rifamycin and gentamycin on EF formation in laminectomized rats. Thirty-two rats were randomly and equally divided into four groups as follows: laminectomy and physiological saline (0.9% NaCl) solution (control); laminectomy and rifamycin; laminectomy and gentamicin; and laminectomy and a mixture of rifamycin and gentamicin. Laminectomy was performed on L1 and L2 vertebrae in all rats. One month after spinal surgery, spinal tissue samples surrounding the laminectomy were cut with a microtome and stained with hematoxylin-eosin and Masson’s trichrome. The histopathological analysis included examining the extent of EF, fibroblast cell density, and cartilage and bone regeneration. Statistical analysis was performed using the IBM SPSS Statistics 22 program (SPSS IBM, Turkey). A value of p < 0.05 was considered statistically significant. EF value differences between the AB treatment groups and the control group were statistically significant (p = 0.030). Specifically, binary comparisons indicated that the EF value was significantly higher in the rifamycin group than that in the control group (p = 0.003; p < 0.05). Our study suggests that locally applied ABs, especially rifamycin, should be diluted before administration to the epidural space.

Key Words

epidural fibrosis failed back surgery syndrome gentamicin inflammation irrigation solution prophylaxis rifamycin spinal infections 


Funding Information

The present study was supported partly by the revolving fund of the Istanbul Fatih Sultan Mehmet Education and Research Hospital at the University of Health Sciences.

Compliance with Ethical Standards

Ethical Approval

“All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.”

Conflict of Interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Neurological Surgery, Istanbul Fatih Sultan Mehmet Education and Research HospitalUniversity of Health Sciencesİcerenkoy-AtasehirTurkey
  2. 2.Department of Neurological Surgery, Haydarpasa Numune Education and Research HospitalUniversity of Health SciencesIstanbulTurkey
  3. 3.Department of Pathology, Istanbul Fatih Sultan Mehmet Education and Research HospitalUniversity of Health SciencesIstanbulTurkey

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