, Volume 42, Issue 2, pp 586–597 | Cite as

Hydrogen-Rich Saline Ameliorates Experimental Autoimmune Encephalomyelitis in C57BL/6 Mice Via the Nrf2-ARE Signaling Pathway

  • Yaping Liu
  • Fuxing Dong
  • Rui Guo
  • Ying Zhang
  • Xuebin Qu
  • Xiuxiang Wu
  • Ruiqin YaoEmail author


Multiple sclerosis (MS) is a chronic and inflammatory disease of the central nervous system that is associated with demyelination, neurodegeneration, and sensitivity to oxidative stress. Hydrogen-rich saline (HRS) is efficacious in preventive and therapeutic applications for many disorders because of its antioxidant and anti-inflammatory properties. Here, we determined the effect of HRS in experimental autoimmune encephalomyelitis (EAE), which is a generally accepted model of the immuno-pathogenic mechanisms underlying MS. We found that HRS reduced the severity of EAE in mice and alleviated inflammation and demyelination. Furthermore, treatment with HRS attenuated oxidative stress in EAE mice. Finally, the results of our study suggest that activation of the Nrf2-ARE pathway plays a critical role in the protective effects of HRS in EAE mice.


multiple sclerosis experimental autoimmune encephalomyelitis hydrogen-rich saline oxidative stress nrf2-ARE 



We sincerely thank Professor Xuejun Sun from the Second Military Medical University for his help with hydrogen-rich saline preparation.

Funding Information

This work was supported by the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (15KJB180018, 18KJB180028) and the Science and Technology Project of Xuzhou City (KC16SH076).

Compliance with Ethical Standards

All animal care and experimental procedures were carried out according to the National Institutes of Health Guide for the Care and Use of Laboratory Animals and were approved by the Bioethics Committee of Xuzhou Medical University.

Conflict of Interest

The authors declare that they have no conflicts of interest.


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Laboratory of National Experimental Teaching and Demonstration Center of Basic MedicineXuzhou Medical UniversityXuzhouChina
  2. 2.Research Center for NeurobiologyXuzhou Medical UniversityXuzhouChina
  3. 3.Research Facility Center for MorphologyXuzhou Medical UniversityXuzhouChina
  4. 4.Department of Cell Biology and NeurobiologyXuzhou Medical UniversityXuzhouChina

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