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Altered Cyclooxygenase-2 Expression in Pulmonary Sarcoidosis is not Related to Clinical Classifications

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Abstract

Elevated COX-2 activity is associated with the development of chronic lung diseases leading to bronchial obstruction, including sarcoidosis. The aim of the study was to examine expression pattern of COX-2 messenger RNA (mRNA). Expression was performed by q-PCR method in bronchoalveolar lavage (BAL) cells and peripheral blood (PB) lymphocytes in sarcoidosis patients (n = 61) and control group (n = 30). Analysis of COX-2 mRNA expression level in BAL fluid and PB revealed downregulation in sarcoidosis and control groups. In PB lymphocytes, the statistically significant difference between patients and controls was observed (P = 0.003, Mann–Whitney U test), with higher expression in patients. There were no statistically significant differences between patients without and with parenchymal involvement (stages I vs. II–IV), between patients with acute vs. insidious onset of disease and between patients with abnormal vs. normal spirometry (P > 0.05, Mann–Whitney U test). Results suggest that expression of COX-2 mRNA in patients with pulmonary sarcoidosis is not related to clinical classifications.

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Acknowledgments

This work was financed by the Medical Univeristy of Lodz, number of subsidy: 503/1-151/-4/503-11-002.

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Authors and Affiliations

  1. Department of Molecular Bases of Medicine, 1st Chair of Internal Diseases, Medical University of Lodz, St. Pomorska 251, 92-213, Lodz, Poland

    Justyna Kiszałkiewicz, Dorota Pastuszak-Lewandoska, Daria Domańska-Senderowska, Monika Migdalska-Sęk, Karolina H. Czarnecka, Ewa Nawrot & Ewa Brzeziańska-Lasota

  2. Department of Pneumonology and Allergy, 1st Chair of Internal Diseases, Medical University of Lodz, St. Kopcińskiego 22, Lodz, 90-153, Poland

    Wojciech J. Piotrowski, Paweł Górski & Witold Górski

  3. Department of General and Oncological Pulmonology, 1st Chair of Internal Diseases, Medical University of Lodz, St. Kopcińskiego 22, Lodz, 90-153, Poland

    Adam Antczak

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  1. Justyna Kiszałkiewicz
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Corresponding author

Correspondence to Ewa Brzeziańska-Lasota.

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The study was approved by the Ethics Committee at the Medical University of Lodz (RNN/141/10/KE). Written informed consent was obtained from each patient.

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The authors declare that they have no conflict of interest.

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Kiszałkiewicz, J., Piotrowski, W.J., Pastuszak-Lewandoska, D. et al. Altered Cyclooxygenase-2 Expression in Pulmonary Sarcoidosis is not Related to Clinical Classifications. Inflammation 39, 1302–1309 (2016). https://doi.org/10.1007/s10753-016-0362-y

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