Spatial and temporal patterns of diatom assemblages, and their drivers, in four US streams: evidence from a long-term dataset
Bioassessment to evaluate stream integrity and determine changes related to point-source discharges is typically focused in wadeable streams, with limited understanding of seasonal and annual variation. We used a multi-year (n = 13), multi-site (n = 5–7), seasonally (spring and fall) sampled dataset to evaluate spatial and temporal patterns in diatom assemblages relative to measured environmental variables, land use, and pulp and paper mill discharges in a wadeable stream (Codorus Creek, PA) and three non-wadeable rivers (Leaf River, MS; McKenzie and Willamette rivers, OR). Analysis of variance (ANOVA) and permutational ANOVA (PERMANOVA) showed that significant spatial differences in commonly used diatom biotic integrity/diagnostic metrics and assemblage structure were common in the wadeable stream, but rare in the non-wadeable rivers. Season-specific diatom patterns were observed in all streams regardless of size, but annual variation was more prevalent in the non-wadeable rivers. Environmental variables explained 35–58% of the variability in diatoms in the spring and 33–50% in the fall, with physical habitat characteristics associated with stream morphology and seasonality more important than those associated with anthropogenic inputs such as land use and point sources. Findings from this study highlight the value of spatially and temporally comprehensive datasets in understanding and interpreting diatom assemblage patterns.
KeywordsBioassessment Benthic diatom Non-wadeable stream Wadeable stream Seasonal variation Annual variation Pulp and paper mill effluent
Assistance with periphyton sampling over the course of the study was provided by D. Brodhecker, F. Howell, A. O’Brien, R. Philbeck, N. Frum, J. Napack, G. Allen, C. Erickson, K. Ramage, J. Redmond, J. Thomas, and M. Cody. Samples were processed with assistance from J. Redmond. Y. Pan ensured that diatom identifications were accurate and reflected the most current taxonomic nomenclature. Discussions with M. Dubé, S. Holm, W. Landis, W. Minshall, J. Rodgers, and S. Missimer on study design and analysis were valuable. S. Courtenay provided guidance on data analyses and manuscript development. M. Harris prepared site maps, and S. Easthouse assisted with manuscript editing and formatting. Valuable insight and feedback provided by two anonymous reviewers improved the manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Anderson, M. J., R. N. Gorley & K. R. Clarke, 2008. PERMANOVA+ for PRIMER: Guide to Software and Statistical Methods. PRIMER-E, Plymouth.Google Scholar
- Association of Clean Water Administrators (ACWA), 2012. Use of Biological Assessment in State Water Programs: Focus on Nutrients. ACWA, Washington, DC.Google Scholar
- Bahls, L. L., 1993. Periphyton Bioassessment Methods for Montana Streams. Water Quality Bureau, Department of Health and Environmental Services, Helena, MT.Google Scholar
- Barbour M., J. Gerritsen, B. D. Snyder & J.B. Stribling, 1999. Rapid bioassessment protocols for use in streams and wadeable rivers: periphyton, benthic macroinvertebrates and fish. EPA 841-B-99-002, 3rd ed. U.S. Environmental Protection Agency, Office of Water, Washington, DCGoogle Scholar
- Chapman, D. V., 1996. Water Quality Assessments: A Guide to the Use of Biota, Sediments and Water in Environmental Monitoring, 2nd ed. United Nations Educational, Scientific and Cultural Organization; World Health Organization; and United Nations Environment Programme, London: 626.CrossRefGoogle Scholar
- Clarke, K. R., R. N. Gorley, P. J. Somerfield & R. M. Warwick, 2014. Change in Marine Communities: An Approach to Statistical Analysis and Interpretation, 3rd ed. PRIMER-E, Plymouth.Google Scholar
- Culp, J. M. & C. L. Podemski, 1996. Design and application of a novel stream microcosm system for assessing effluent impacts to large rivers. In Servos, M. R., K. R. Munkittrick, J. H. Carey & G. J. Van Der Kraak (eds), Environmental fate and effects of pulp and paper mill effluents. Delray Beach (FL), St. Lucie: 549–555.Google Scholar
- Environment Canada, 1992. Pulp and paper effluent regulations. Canada Gazette 126 (11, part II):1967–1997.Google Scholar
- Environment Canada, 2010. 2010 Pulp and Paper Environmental Effects Monitoring (EEM) Technical Guidance Document. Environment Canada, Ottawa: 490.Google Scholar
- Flinders, C. A., G. W. Minshall, T. J. Hall & J. H. Rodgers, 2009a. Spatial and temporal patterns of periphyton chlorophyll a related to pulp and paper mill discharges in four US receiving streams. Integrated Environmental Assessment and Management 5: 264–274.Google Scholar
- Flinders, C. A., R. L. Ragsdale & T. J. Hall, 2009b. Patterns of fish community structure in a long-term watershed-scale study to address the aquatic ecosystem effects of pulp and paper mill discharges in four US receiving streams. Integrated Environmental Assessment and Management 5: 224–238.Google Scholar
- Franklin, J. F., 1988. Importance and justification of long-term studies in ecology. In Likens, G. E. (ed.), Long-term Studies in Ecology: Approaches and Alternatives. Springer, New York: 3–19.Google Scholar
- Hall, T. J., R. P. Fisher, J. L. Rodgers, G. W. Minshall, W. G. Landis, T. G. Kovacs, B. K. Firth, M. G. Dubé, T. L. Deardorff & D. L. Borton, 2009. A long-term multitrophic level study to assess pulp and paper mill effluent effects on aquatic communities in four United States receiving waters: background and status. Integrated Environmental Assessment Management 5: 189–198.CrossRefGoogle Scholar
- Jarvie, H. P., C. Neal, R. Smart, R. Owen, D. Fraser, I. Forbes & A. Wade, 2001. Use of continuous water quality records for hydrograph separation and to assess short-term variability and extremes in acidity and dissolved carbon dioxide for the River Dee, Scotland. The Science of the Total Environment 265: 85–98.CrossRefGoogle Scholar
- Kovacs T.G., P. H. Martel, B. I. O’Conner, J. S. Gibbons & R. H. Voss, 2003. Effluent-related benefits derived from process and treatment changes implemented by the Canadian pulp and paper industry in the 1990s. In Stuthridge T. R., M. R. van den Heuvel, N. A. Marvin, A. H. Slade & J. Gifford (eds) Environmental impacts of pulp and paper waste streams: Proceedings of the Third International Conference on Environmental Fate and Effects of Pulp and Paper Effluents; 1997 Nov 9–13; Rotorua (NZ): 238–248Google Scholar
- Krammer, K. & H. Lange-Bertalot, 1986. Bacillariophyceae, Teil 1. Naviculaceae. Spektrum Akademischer Verlag, Heidelberg.Google Scholar
- Krammer, K. & H. Lange-Bertalot, 1988. Bacillariophyceae, Tiel 2. Bacillariophyceae, Epithemiaceae, Surirellaceae. In Ettl, H., J. Gerloff, H. Heynig & D. Mollenhauer (eds), Süsswasserflora von Mitteleuropa. Spektrum Akademischer Verlag, Heidelberg: 1–876.Google Scholar
- Krammer, K. & H. Lange-Bertalot, 1991a. Bacillariophyceae, Teil 3. Centrales, Fragilariaceae, Eunotiaceae, Achnanthaceae. Spektrum Akademischer Verlag, Heidelberg.Google Scholar
- Krammer, K. & H. Lange-Bertalot, 1991b. Bacillariophyceae, Teil 4. Achnanthaceae, kritsche Erganzungen zu Navicula (Lineolatae) und Gomphonema Gesamtliteraturverzeichnis, Tiel 1–4. Spektrum Akademischer Verlag, Heidelberg Germany.Google Scholar
- Lange-Bertalot, H., 1979. Diatomeen-differentialarten anstelle von leitformen – Ein geeigneteres kriterium der gewasserbelastung. Archives Hydrobiologia Supplement 51: 393–427.Google Scholar
- Luxon, M. & W. G. Landis, 2005. Application of the relative risk model to the upper Willamette River and lower McKenzie River, Oregon. In Landis, W. G. (ed.), Regional Scale Ecological Risk Assessment Using the Relative Risk Model. CRC, Boca Raton, FL: 91–118.Google Scholar
- Martel, P.H., B. I. O’Connor, T.G. Kovacs, M. R. van den Heuvel, J. L. Parrott, M.E. McMaster, D. L. MacLatchy, G. J. Van Der Kraak & L. M. Hewitt, 2017. The relationship between organic loading and effects on fish reproduction for pulp mill effluents across Canada. Environmental Science & Technology 51: 3499–3507.CrossRefGoogle Scholar
- NCASI (National Council for Air and Stream Improvement), 2000. An Update of Procedures for the Measurement of Color in Pulp Mill Wastewaters. Technical Bulletin No. 803. National Council for Air and Stream Improvement, Inc.: Research Triangle Park NC.Google Scholar
- Oelsner, G. P., L. A. Sprague, J. C. Murphy, R. E. Zuellig, H. M. Johnson, K. R. Ryberg, J. A. Falcone, E. G. Stets, A. V. Vecchia, M. L. Riskin, L. A. De Cicco, T. J. Mills & W. H. Farmer, 2017. Water-quality trends in the Nation’s Rivers and Streams, 1972-2012-Data Preparation, Statistical Methods, and Trend Results. U.S. Geological Survey Scientific Investigations Report 2017-5006. https://doi.org/10.3133/sir20175006.
- Patrick, R. & C. W. Reimer, 1966. The Diatoms of the United States. Volume 1. Monographs of the Academy of Natural Sciences of Philadelphia, no. 13. Academy of Natural Sciences of Philadelphia, Philadelphia, PA. 688 p.Google Scholar
- Patrick, R. & C. W. Reimer, 1975. The Diatoms of the United States. Volume 2. Monographs of the Academy of Natural Sciences of Philadelphia, no. 13. Academy of Natural Sciences of Philadelphia, Philadelphia, PA. 213 p.Google Scholar
- Podemski, C. L. & J. M. Culp, 1996. Nutrient and contaminant effects of bleached kraft mill effluent on benthic algae and insects of the Athabasca River. In Servos, M. R., K. R. Munkittrick, J. H. Carey & G. J. Van Der Kraak (eds), Environmental Fate and Effects of Pulp and Paper Mill Effluents. Delray Beach (FL), St. Lucie: 571–580.Google Scholar
- Porter, S. D., 2008. Algal Attributes: An Autecological Classification of Algal Taxa Collected by the National Water-Quality Assessment Program: U.S. Geological Survey Data Series 329, http://pubs.usgs.gov/ds/ds329/.
- Rosemond, A. D., P. J. Mulholland & S. H. Brawley, 2000. Seasonally shifting limitation of stream periphyton: response of algal populations and assemblage biomass and productivity to variation in light, nutrients, and herbivores. Canadian Journal of Fisheries and Aquatic Sciences 57: 66–75.CrossRefGoogle Scholar
- Snell, M. A., P. A. Barker, B. W. J. Surridge, C. McW. H. Benskin, N. Barber, S. M. Reaney, W. Tych, D. Mindham, A. R. G. Large, S. Burke & P. M. Haygarth, 2019. Strong and recurring seasonality revealed within stream diatom assemblages. Scientific Reports 9: Article number: 3313.Google Scholar
- Spaulding, S.A., I.W. Bishop, M.B. Edlund, S. Lee, P. Furey, E. Jovanovska & M. Potapova, 2019. Diatoms of North America. https://diatoms.org/
- Thomas, J., 2003. Integration of a relative risk multi-stressor risk assessment with the NCASI long-term receiving water studies to assess effluent effects at the watershed level, Leaf River, Mississippi. Technical Bulletin 867. National Council for Air and Stream Improvement: Research Triangle Park NC.Google Scholar
- United States Environmental Protection Agency (US EPA), 1983. Methods for Chemical Analysis of Water and Wastes, EPA-600/4-79-020. USEPA Office of Research and Development, Washington DC.Google Scholar
- US EPA. 2006. Final report: Pulp and paper and paperboard detailed study. Office of Water, Washington, DC. EPA-821-R-06-016.Google Scholar
- US EPA, 2016. National Rivers and Streams Assessment 2008-2009: A Collaborative Survey, EPA/841/R-16/007. Office of Water and Office of Research and Development, Washington, DC.Google Scholar
- Wieczorek, M. E., & A. E. LaMotte, 2010. Attributes for NHDPlus catchments (version 1.1) for the conterminous United States: NLCD 2001 land use and land cover. U.S. Geological Survey Digital Data Series DDS-490-15. http://water.usgs.gov/GIS/metadata/usgswrd/XML/nhd_nlcd01.xml. Accessed Jan 2018.