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Effect of two commercial probiotic products on population growth of rotifer Brachionus rotundiformis Tschugunoff

  • Robert Nesta Kagali
  • Hee-Jin Kim
  • Terumi Koga
  • Yoshitaka Sakakura
  • Atsushi Hagiwara
ROTIFERA XV

Abstract

The application of probiotics has varying effects on rotifer culture. These effects can either be directly on their growth and reproduction or indirectly by affecting the culture environment. The role played by specific probiotic candidates is still indistinct and further research is required to ascertain their significance in rotifer culture. In this study, euryhaline rotifer Brachionus rotundiformis (SS-type) was used to evaluate the effect of two commercial probiotic products on rotifer density, reproductive parameters and unionized ammonia concentration for a twelve-day culture period. To estimate these parameters, rotifers were cultured in glass bottles containing dilute sterilized sea water with the following five diet treatments: Nannochloropsis oculata (control), Igsign (PB1), Toaraze Aqua (PB2) or the combination of N. oculata with either PB1 or PB2. The rotifer density was significantly affected by the addition of probiotic. N. oculata + PB2 treatment resulted in higher rotifer density and specific growth rate compared to the control treatment. Mixis induction was unaffected by N. oculata + PB2 while it was repressed by N. oculata + PB1. Ammonia concentration was not significantly affected with the addition of probiotics. These results indicate that probiotics have synergetic effects with microalgae diet that resulted in the highest rotifer densities.

Keywords

Euryhaline rotifer Probiotic Mixis Density Microalgae 

Notes

Acknowledgements

This research was partly supported by Japanese Society for Promotion of Sciences (JSPS) KAKENHI Grant number JP17H038662 to Atsushi Hagiwara as well as Japan International Cooperation Agency (JICA) which provided Ph.D. fellowship to Robert N. Kagali under the JKUAT/PAUSTI/AU Network project_Nagasaki University (J1610573). The commercial probiotic products used in this research were provided by Toa pharmaceutical company Limited, Tokyo, Japan.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

10750_2018_3852_MOESM1_ESM.docx (35 kb)
Supplementary material 1 (DOCX 35 kb)
10750_2018_3852_MOESM2_ESM.doc (113 kb)
Supplementary material 2 (DOC 113 kb)

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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  1. 1.Graduate School of Fisheries and Environmental SciencesNagasaki UniversityNagasakiJapan
  2. 2.Department of ZoologyJomo Kenyatta University of Agriculture and TechnologyNairobiKenya
  3. 3.Toa Pharmaceutical Company LimitedTokyoJapan

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