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Hydrobiologia

, Volume 830, Issue 1, pp 17–31 | Cite as

Cyanobacterial diversity in the algal–bacterial consortia from Subarctic regions: new insights from the rock baths at White Sea Coast

  • A. Kublanovskaya
  • K. ChekanovEmail author
  • A. Solovchenko
  • E. Lobakova
Primary Research Paper

Abstract

Cyanobacteria characterized by exceptional tolerance to environmental stresses often become pioneer settlers in habitats with harsh conditions. There, they can constitute the core of microbial communities. The taxonomic composition of the cyanobacterial component of algal–bacterial consortia dwelling in a habitat with particularly harsh conditions (rock baths at the coast of Kandalaksha Bay of the White Sea) has been elucidated for the first time. Two workflows of the taxonomic analysis of the cyanobacteria were tested including the combinations of two programs and two databases (QIIME+Greengenes and Usearch+NCBI GenBank). Our results obviated the need of the using of a complex approach combining morphological and metagenomic analyses for revealing the taxonomic structure of cyanobacteria in natural habitats. Our results show that the cyanobacterial component of the consortia from the habitats with harsh and highly volatile environmental conditions is enriched with non-diazotrophic and diazotrophic non-branched filamentous cyanobacteria.

Keywords

Algal–bacterial consortia Cyanobacteria Metagenome Polyphasic approach 

Abbreviations

16SrRNA

Gene of 16S ribosomal RNA

NJ

Neighbor-joining algorithm

PCR

Polymerase chain reaction

OTU

Operation taxonomic unit

QIIME+GG

Workflow of sequence data analysis in QIIME software and homolog search in the Greengenes database

Usearch+GB

Workflow of sequence data analysis in Usearch software and homolog search in the GenBank NCBI database

Notes

Acknowledgements

Microscopic studies were carried out using equipment at the Center of Microscopy of White Sea Biological Station of Moscow State University. This work was supported partially by the Ministry of Science and Education of the Russian Federation (Grant Number 02.a03.21.0008 of 24 June 2016) and partially by Council for Grants of the President of the Russian Federation. The dedicated technical assistance of Dr. Yuriy Khlopko is greatly appreciated.

Supplementary material

10750_2018_3844_MOESM1_ESM.pdf (481 kb)
Supplementary material 1 (PDF 481 kb)

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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  1. 1.Department of Bioengineering, Faculty of BiologyLomonosov Moscow State UniversityMoscowRussia
  2. 2.Centre for Humanities Research and TechnologyNational Research Nuclear University MEPhiMoscowRussia
  3. 3.Eurasian Center for Food SecurityLomonosov Moscow State UniversityMoscowRussia

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