Cyanobacterial diversity in the algal–bacterial consortia from Subarctic regions: new insights from the rock baths at White Sea Coast
Cyanobacteria characterized by exceptional tolerance to environmental stresses often become pioneer settlers in habitats with harsh conditions. There, they can constitute the core of microbial communities. The taxonomic composition of the cyanobacterial component of algal–bacterial consortia dwelling in a habitat with particularly harsh conditions (rock baths at the coast of Kandalaksha Bay of the White Sea) has been elucidated for the first time. Two workflows of the taxonomic analysis of the cyanobacteria were tested including the combinations of two programs and two databases (QIIME+Greengenes and Usearch+NCBI GenBank). Our results obviated the need of the using of a complex approach combining morphological and metagenomic analyses for revealing the taxonomic structure of cyanobacteria in natural habitats. Our results show that the cyanobacterial component of the consortia from the habitats with harsh and highly volatile environmental conditions is enriched with non-diazotrophic and diazotrophic non-branched filamentous cyanobacteria.
KeywordsAlgal–bacterial consortia Cyanobacteria Metagenome Polyphasic approach
Gene of 16S ribosomal RNA
Polymerase chain reaction
Operation taxonomic unit
Workflow of sequence data analysis in QIIME software and homolog search in the Greengenes database
Workflow of sequence data analysis in Usearch software and homolog search in the GenBank NCBI database
Microscopic studies were carried out using equipment at the Center of Microscopy of White Sea Biological Station of Moscow State University. This work was supported partially by the Ministry of Science and Education of the Russian Federation (Grant Number 02.a03.21.0008 of 24 June 2016) and partially by Council for Grants of the President of the Russian Federation. The dedicated technical assistance of Dr. Yuriy Khlopko is greatly appreciated.
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