, Volume 827, Issue 1, pp 367–378 | Cite as

Body size and population dynamics of annual fishes from temporary wetlands in Southern Brazil

  • Matheus V. VolcanEmail author
  • Ândrio C. Gonçalves
  • Demetrio Luis Guadagnin
Primary Research Paper


Annual fishes live exclusively in temporary wetlands where several species coexist. Understanding how annual fishes cohabit in small and isolated pools is still a challenge. In this study, we aimed to examine the prediction that annual fish assemblages in temporary pools in Southern Brazil are structured by temporal differences in body size, specific growth rate and density. Two–three fish species coexisted in each pool. The most abundant and frequent species were Cynopoecilus melanotaenia and Austrolebias nigrofasciatus, while A. wolterstorffi was less abundant and rare. The species differed significantly in body size throughout the flooding phase and showed similar growth patterns, with higher specific growth rates in the first months of flooding and a reduction in growth rate thereafter. The monthly average densities of A. nigrofasciatus and C. melanotaenia did not differ over time, but they were higher than values observed for A. wolterstorffi. Our results provide evidence for hatching synchrony, similar specific growth rates and densities among the most abundant species and differences in body sizes among all species that are more evident after adulthood, suggesting that differences in body size mediate the coexistence of annual fishes in temporary pools.


Killifish Austrolebias Cynopoecilus Specific growth rate Density Seasonal pool 



The authors thank Alinca P. Fonseca and Luis Esteban K. Lanés for their support in the fish collection. The authors also thank Michel Corrêa for providing the map of the study area, and ICMBio for obtaining the license (28099-1). M.V. Volcan thanks CAPES for the Ph.D. Grant. CNPq provided research fellowship to D. L. Guadagnin (309298/2009-1).

Supplementary material

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Supplementary material 1 (PDF 471 kb)
10750_2018_3789_MOESM2_ESM.pdf (437 kb)
Supplementary material 2 (PDF 436 kb)


  1. Almirón, A., J. Casciotta, P. Giorgis & L. Ciotek, 2008. Guía de los peces del Parque Nacional Pre-Delta. Administración de Parques Nacionales. 1ª. Ed. Buenos Aires.Google Scholar
  2. Arenzon, A., A. C. Peret & M. B. C. Bohrer, 1999. Reproduction of the annual fish Cynopoecilus maelanotaenia (Regan 1912) based on a temporary water body population in Rio Grande do Sul State, Brazil. Hydrobiologia 411: 65–70.CrossRefGoogle Scholar
  3. Arim, M., S. B. Abades, G. Laufer, M. Loureiro & P. Marquet, 2009. Food web structure and body size trophic position and resource acquisition. Oykos 119: 147–153.CrossRefGoogle Scholar
  4. Bates, D., M. Maechler, B. Bolker & S. Walker, 2015. _lme4: Linear mixed-effects models using Eigen and S4_. R package version 1.1-8. [available on internet at
  5. Blažek, R., M. Reichard & M. Polačik, 2013. Rapid growth, early maturation and short generation time in African annual fishes. EvoDevo 4: 24.CrossRefGoogle Scholar
  6. Bolker, B. M., M. E. Brooks, C. J. Clark, S. W. Geange, J. R. Poulsen, M. H. H. Stevens & J. S. S. White, 2009. Generalized linear mixed models: a practical guide for ecology and evolution. Trends in Ecology and Evolution 24: 127–135.CrossRefGoogle Scholar
  7. Canavero, A., D. Hernández, M. Zarucki & M. Arim, 2014. Patterns of co-occurrences in a killifish metacommunity are more related with body size than with species identity. Austral Ecology 39: 455–461.CrossRefGoogle Scholar
  8. Casciotta, J., A. Almirón & J. Bechara, 2005. Peces del Iberá: Hábitat y Diversidad. Fundación Ecos, 1ª ed. Corrientes, Argentina.Google Scholar
  9. Cohen, J. E., J. E. Cohen, T. Jonsson & S. R. Carpenter, 2003. Ecological community description using the food web, species abundance, and body size. Proceedings of the National Academy of Sciences 100: 1781–1786.CrossRefGoogle Scholar
  10. Collinson, N. H., J. Biggs, A. Corfield, M. J. Hodson, D. Walker, M. Whitfield & P. J. Williams, 1995. Temporary and permanent ponds: an assessment of the effects of drying out on the conservation value of aquatic macroinvertebrate communities. Biological Conservation 74: 125–133.CrossRefGoogle Scholar
  11. Córdova-Tapia, F., V. Hernández-Marroquín & L. Zambrano, 2017. The role of environmental filtering in the functional structure of fish communities in tropical wetlands. Ecology of Freshwater Fishes 2018(27): 522–532.Google Scholar
  12. Costa, W. J. E. M. 2002. Peixes Anuais Brasileiros: Diversidade e Conservação. Curitiba, ed. UFPR.Google Scholar
  13. Costa, W. J. E. M., 2006. The South American annual killifish genus Austrolebias (Teleostei: Cyprinodontiformes: Rivulidae): phylogenetic relationships, descriptive morphology and taxonomic revision. Zootaxa 1213: 1–162.Google Scholar
  14. Costa, W. J. E. M., 2009. Trophic radiation in the South American annual killifish genus Austrolebias (Cyprinodontiformes: Rivulidae). Ichthyological Explorations Freshwaters 20: 179–191.Google Scholar
  15. Embrapa, 2012. Embrapa Clima Temperado: Agritempo Pelotas – versão eletrônica. Accessed Jan 3 2013.
  16. Errea, A. & E. Danulat, 2001. Growth of the annual fish, Cynolebias viarius (Cyprinodontiformes), in the natural habitat compared to laboratory conditions. Environmental Biology of Fishes 61: 261–268.CrossRefGoogle Scholar
  17. Ellis, T. & R. N. Gibson, 1995. Size-selective predation of 0-group flatfishes on a Scottish coastal nursery ground. Marine Ecology Progress Series 127: 27–37.CrossRefGoogle Scholar
  18. Fonseca, A. P., M. V. Volcan, L. A. Sampaio, L. A. Romano & R. B. Robaldo, 2013. Growth of critically endangered anual fish Austrolebias wolterstorffi (Cyprinodontiformes: Rivulidae) at different temperatures. Neotropical Ichthyology 11: 837–844.CrossRefGoogle Scholar
  19. Fonseca, A. P., M. V. Volcan & R. B. Robaldo, 2018. Incubation media affect the survival, pathway and time of embryo development in Neotropical annual fish Austrolebias nigrofasciatus (Rivulidae). Journal of Fish Biology 92: 165–176.CrossRefGoogle Scholar
  20. Gomes S. 1998. A closer look: Wetlands. North Dakota Outdoors, 60(10): 12-13. [available on internet at]. Accessed Jan 2017.
  21. Gonçalves, C. S., U. P. Souza & M. V. Volcan, 2011. The opportunistic feeding and reproduction strategies of the annual fish Cynopoecilus melanotaenia (Cyprinodontiformes: Rivulidae) inhabiting ephemeral habitats on southern Brazil. Neotropical Ichthyology 9(1): 191–200.CrossRefGoogle Scholar
  22. Gonçalves, Â. C. 2013. Influence of habitat and landscape on the occurrence of annual fish (Cyprinodontiformes: Rivulidae). Universidade Federal de Santa Maria, Master thesis.Google Scholar
  23. Houde, E. D., 1987. Fish early life dynamics and recruitment variability. American Fisheries Society Symposium 2: 17–29.Google Scholar
  24. ICMBio, (Instituto Chico Mendes de Conservação da Biodiversidade), 2013. Sumário Executivo do Plano de Ação Nacional para a Conservação dos Peixes Rivulídeos Ameaçados de Extinção. Brasília [available on internet at]. Accessed Apr 14 2016.
  25. Keppeler, F. W., L. E. K. Lanés, A. S. Rolon, C. Stenert, P. Lehmann, M. Reichard & L. Maltchik, 2014. The morphology–diet relationship and its role in the coexistence of two species of annual fishes. Ecology of Freshwater Fish 24: 77–90.CrossRefGoogle Scholar
  26. Kohda, M. & K. Tanida, 1996. Overlapping territory of the benthophagous cichlid fish, Lobochilotes labiatus, in Lake Tanganyika. Environmental Biology of Fishes 45: 13–20.CrossRefGoogle Scholar
  27. Kuwamura, T., 1984. Social structure of the protogynous fish Labroides dimidiatus. Publications of the Seto Marine Biological Laboratory 29: 117–177.  Google Scholar
  28. Lanés, L. E. K., F. W. Keppeler & L. Maltchik, 2014. Abundance variations and life history traits of two sympatric species of Neotropical annual fish (Cyprinodontiformes: Rivulidae) in temporary ponds of southern Brazil. Journal of Natural History 48: 1971–1988.CrossRefGoogle Scholar
  29. Lanés, L. E. K., R. S. Godoy, L. Maltchik, M. Polačik, R. Blažek, M. Vrtílek & M. Reichard, 2016. Seasonal dynamics in community structure, abundance, body size and sex ratio in two species of Neotropical annual fishes. Journal of Fish Biology 89: 2345–2364.CrossRefGoogle Scholar
  30. Laufer, G., M. Arim, M. Loureiro, J. M. Piñero-Guerra, S. Clavijo-Baquet & C. Fagúndez, 2009. Diet of four annual killifishes: an intra and interspecific comparision. Neotropical Ichthyology 7: 77–86.CrossRefGoogle Scholar
  31. Lenth, R. V. 2013. lsmeans: Least-squares means. R package version 1.06-05.
  32. Li, L. & P. Chesson, 2016. The effects of dynamical rates on species coexistence in a variable environment: the paradox of the plankton revisited. American Naturalist 188(2): 45–58.CrossRefGoogle Scholar
  33. Matsumoto, K., 2001. Overlapping territory of a benthophagous fish, Goniistius zonatus (Teleostei: Cheilodactylidae). Ecological Research 16: 715–726.CrossRefGoogle Scholar
  34. Millar, R. B. & M. J. Anderson, 2004. Remedies for pseudoreplication. Fisheries Research 70: 397–407.CrossRefGoogle Scholar
  35. MMA, 2007. Ministerio do Meio Ambiente–Areas Prioritarias para Conservacao, Uso Sustentável e Reparticao dos Benefícios da Biodiversidade Brasileira–Bioma Pampa. Secretaria de Biodiversidade e Florestas. Brasília: Ministério do Meio Ambiente.Google Scholar
  36. Nico, L. G. & J. E. Thomerson, 1989. Ecology, food habits and spatial interactions of Orinoco Basin annual killifish. Acta Biologica Venezuelica 12: 106–120.Google Scholar
  37. Oksanen, J., F. Blanchet, G. Kindt, R. Legendre, P. Minchin, P. R. O’Hara, R. B. Simpson, G. L., P. Solymos, H. Henry, M. Stevens & H. Wagner, 2016. Vegan: community ecology package. R package version 2.3-5.
  38. R Core Team, 2013. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Viena, Austria.
  39. Reichard, M., M. Polačik & O. Sedláček, 2009. Distribution, colour polymorphism and habitat use of the African killifish, Nothobranchius furzeri, the vertebrate with the shortest lifespan. Journal of Fish Biology 74: 198–212.CrossRefGoogle Scholar
  40. Reichard, M., L. E. K. Lanés, M. Polačik, R. Blažek, M. Vrtílek, R. S. Godoy & L. Maltchik, 2018. Avian predation mediates size-specific survival in a Neotropical annual fish: a field experiment. Biological Journal of the Linnean Society 124(1): 56–66.CrossRefGoogle Scholar
  41. Rosa, M. 1985. Geografia de Pelotas. Pelotas: Ed.UFPel.Google Scholar
  42. Rosa, R. S. & F. C. T. Lima, 2008. Peixes. In Machado, A. B. M., G. M. Drummond & A. P. Paglia (eds), Livro vermelho da fauna brasileira ameaçada de extinção. Ministério do Meio Ambiente, Brasília.Google Scholar
  43. Sakai, Y. & M. Kohda, 1997. Harem structure of the protogynous angelfish, Centripyge ferrugatus (Pomacanthidae). Environmental Biology of Fishes 49: 333–339.CrossRefGoogle Scholar
  44. Sogard, S. M., 1997. Size-selective mortality in the Juvenile stage of teleost fishes: a review. Bulletin of Marine Science 60: 1129–1157.Google Scholar
  45. Taborsky, B., Heino, M. & U. Dieckmann, 2012. Size-dependent mortality and competition interactively shape community diversity. Evolution 66: 3534–3544.CrossRefGoogle Scholar
  46. Van der Veer, H. W. & M. J. Bergman, 1987. Predation by crustaceans on a newly settled 0-group plaice Pleuronectes platessa L. population. Marine Ecology Progress Series 35: 203–215.CrossRefGoogle Scholar
  47. Van der Veer, H. W., L. Pihl & M. J. Bergman, 1990. Recruitment mechanisms in North Sea plaice Pleuronectes platessa. Marine ecology progress series. Oldendorf 64: 1–12.CrossRefGoogle Scholar
  48. Vaz-Ferreira, R., B. Sierra de Soriano & J. S. Señorans, 1966. Integracion de La fauna de vertebrados em algunas masas de agua dulce temporales del Uruguay. Compilaciones de los Trabajos del Departamento de Zoologia de Vertebrados 25: 1–16.Google Scholar
  49. Vazzoler, A. E. A. M., 1996. Biologia da reprodução de peixes teleósteos: teoria e prática. EDUEM, Maringá.Google Scholar
  50. Volcan, M. V., L. E. K. Lanés & A. C. Gonçalves, 2009. Threatened fishes of the world: Austrolebias nigrofasciatus Costa and Cheffe 2001 (Cyprinodontiformes: Rivulidae). Environmental Biology of Fishes 86: 319–320.CrossRefGoogle Scholar
  51. Volcan, M. V. L. E. K., M. M. Lanés & M. M. Cheffe, 2010. Distribuição e Conservação de Peixes Anuais (Cyprinodontiformes, Rivulidae) no município do Chuí, Brasil. Biotemas 23: 51–58.CrossRefGoogle Scholar
  52. Volcan, M. V., A. P. Fonseca, M. R. C. Figueiredo, L. A. Sampaio & R. B. Robaldo, 2012. Effect of temperature on growth of the threatened annual fish Austrolebias nigrofasciatus Costa & Cheffe, 2001. Biota Neotropica 12: 68–73.CrossRefGoogle Scholar
  53. Volcan, M. V., Â. C. Gonçalves & D. L. Guadagnin, 2013. Length-weight relationship of three annual fishes (Rivulidae) from temporary freshwater wetlands of southern Brazil. Journal of Applied Ichthyology 29: 1188–1190.CrossRefGoogle Scholar
  54. Volcan, M. V., Â. C. Gonçalves & L. E. K. Lanés, 2014. Austrolebias quirogai (Actinopterygii: Cyprinodontiformes: Rivulidae) in Brazil: occurrence, population parameters, habitat characteristics, and conservation status. Acta Ichthyologica et Piscatoria 44: 37–44.CrossRefGoogle Scholar
  55. Volcan, M. V., L. E. K. Lanés, Â. C. Gonçalves & D. L. Guadagnin, 2015. Annual fishes (Rivulidae) from Southern Brazil: a broad-scale assessment of their diversity and conservation. In Berois, N., G. García & R. O. de Sá (eds), Annual Fishes: Life History Strategy, Diversity, and Evolution. CRC Press Taylor and Francis Group, New York.Google Scholar
  56. Wourms, J. P., 1972. The developmental biology of annual fishes. III. Pre embryonic and embryonic diapause of variable duration in the eggs of annual fishes. Journal of Experimental Zoology 182: 389–414.CrossRefGoogle Scholar

Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  1. 1.Instituto Pró-Pampa (IPPampa), Laboratório de IctiologiaPelotasBrazil
  2. 2.Programa de Pós Graduação em Biodiversidade Animal, Universidade Federal de Santa Maria (UFSM)Santa MariaBrazil
  3. 3.Universidade Federal do Rio Grande do Sul, Depto. de EcologiaPorto AlegreBrazil

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