Expression of Ihh signaling pathway in condylar cartilage after bite-raising in adult rats

  • Hui-qing Long
  • Peng-fei Tian
  • Yu-xin Guan
  • Ling-xia Liu
  • Xiu-ping WuEmail author
  • Bing Li
Original Paper


Temporomandibular joint osteoarthritis (TMJOA) is a complex inflammatory condition with multiple factors and degenerative processes co-occurring. However, its pathogenesis remains uncertain. The purpose of the study was to observe the expression of Indian hedgehog (Ihh) signal related molecules in TMJOA induced by bite-raising and to study the effect and mechanism of Ihh signaling. Our research indicated that Ihh signaling pathway can be activated in condylar cartilage induced by bite-raising. The histological analysis showed TMJOA-like structural changes of condylar cartilage in experiment groups. Ihh, Smoothened (Smo), and Gli zinc finger transcription factors-1 (Gli-1) were activated in the experimental groups, and the expression levels increased significantly over time, whereas the sham control groups showed no fluctuation. Additionally, the expression levels of matrix metalloproteinase-13 (MMP-13) and cysteinyl aspartate specific proteinase-3 (Caspase-3) in the experiment groups increased in a time-dependent manner compared with the matched sham control groups. In conclusion, our results indicated that the Ihh signaling pathway may activate the occurrence of TMJOA by mediating the hypertrophy of chondrocytes, which may be an important regulatory mechanism and potential therapeutic target in the repair of condylar cartilage.


Bite-raising Condylar cartilage Ihh signal related molecules TMJOA 



This Project was supported by the Provincial Key Research and Development Project of Shanxi Province (Grant Number 201803D31065).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Hui-qing Long
    • 1
  • Peng-fei Tian
    • 1
  • Yu-xin Guan
    • 1
  • Ling-xia Liu
    • 1
  • Xiu-ping Wu
    • 1
    Email author
  • Bing Li
    • 1
  1. 1.Department of Orthodontics, School of DentistryShanxi Medical UniversityTaiyuanChina

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