Akt plays indispensable roles during the first cell lineage differentiation of mouse
The first cell lineage differentiation occurs during the development of mouse 8-cell embryo to blastocyst. Akt is a potent kinase whose role during blastocyst formation has not been elucidated. In the present study, immunofluorescence results showed that the Akt protein was specifically localized to the outer cells of the morula. Akt-specific inhibitor MK2206 significantly inhibited mouse blastocyst formation and resulted in decreased expression of the trophectoderm marker Cdx2 and led to granular distribution of ERα in the cytoplasm. Furthermore, knockdown of ERα by siRNA microinjection can also lead to a decrease in the development rate of mouse blastocysts, accompanied by a decrease in the expression level of Yap protein. We conclude that Akt may be indispensable for the first cell lineage differentiation of mouse.
KeywordsAkt Blastocyst formation Yap Immunofluorescence Mouse
This work was supported by grants from National Natural Science Foundation of China (81671526), Fujian Provincial Science and Technology Innovation Joint Fund Project (2017Y9114), Fujian Provincial Project of Education and Science for Young and Middle-aged Teachers (JAT170212), Fujian Provincial Health and Family Planning Research Talents Training Project (2017-1-67) and Science Fund for Distinguished Young Scholars of Fujian Province University (2018B028).
Compliance with ethical standards
Conflict of interest
None of the authors have any conflict of interest to declare.
- Britschgi A, Duss S, Kim S, Couto JP, Brinkhaus H, Koren S, De Silva D, Mertz KD, Kaup D, Varga Z, Voshol H, Vissieres A, Leroy C, Roloff T, Stadler MB, Scheel CH, Miraglia LJ, Orth AP, Bonamy GM, Reddy VA, Bentires-Alj M (2017) The Hippo kinases LATS1 and 2 control human breast cell fate via crosstalk with ERalpha. Nature 541(7638):541–545CrossRefGoogle Scholar
- Chen J, Lian X, Du J, Xu S, Wei J, Pang L, Song C, He L, Wang S (2016) Inhibition of phosphorylated Ser473-Akt from translocating into the nucleus contributes to 2-cell arrest and defective zygotic genome activation in mouse preimplantation embryogenesis. Dev Growth Differ 58(3):280–292CrossRefGoogle Scholar
- Fayard E, Xue G, Parcellier A, Bozulic L, Hemmings BA (2010) Protein kinase B (PKB/Akt), a key mediator of the PI3 K signaling pathway. Curr Top Microbiol Immunol 346:31–56Google Scholar
- Tsukamoto S, Hara T, Yamamoto A, Ohta Y, Wada A, Ishida Y, Kito S, Nishikawa T, Minami N, Sato K, Kokubo T (2013) Functional analysis of lysosomes during mouse preimplantation embryo development. J Reprod Dev 59(1):33–39Google Scholar