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Journal of Molecular Histology

, Volume 49, Issue 6, pp 631–637 | Cite as

First ovarian response to gonadotrophin stimulation in rats exposed to neonatal androgen excess

  • Rebeca Chávez-Genaro
  • Gabriel Anesetti
Original Paper

Abstract

This study analyzes the effects of neonatal androgenization on follicular growth and first ovulation in response to gonadotrophins, using a model of exogenous stimulation or the use of subcutaneous ovary grafts in castrated animals to replace the hypothalamus–pituitary signal. Neonatal rats (days 1–5) were treated with testosterone, dihydrotestosterone or vehicle. At juvenile period, rats were stimulated with PMSG, hCG (alone or combined) or used as ovarian donors to be grafted on castrated adult female rats. Ovulation and ovarian histology were analyzed in both groups. Animals treated with vehicle or dihydrotestosterone stimulated with gonadotrophins (pharmacological or by using an ovary graft) ovulated, showing a normal histological morphology whereas rats exposed to testosterone and injected with the same doses of gonadotrophins did not it. In this group, ovulation was reached using a higher dose of hCG. Ovaries in the testosterone group were characterized by the presence of follicles with atretic appearance and a larger size than those observed in control or dihydrotestosterone groups. A similar appearance was observed in testosterone ovary grafts although luteinization and some corpora lutea were also identified. Our findings suggest that neonatal exposure to aromatizable androgens induces a more drastic signalling on the ovarian tissue that those driven by non-aromatizable androgens in response to gonadotrophins.

Keywords

Testosterone Dihydrotestosterone Follicle Ovulation Graft hCG 

Notes

Acknowledgements

We gratefully acknowledge to Karina Hernandez for histotechnical assistance and Mariela Santos for animal care support.

Funding

This work was partially supported by the PEDECIBA, Universidad de la República, Montevideo, Uruguay.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Histology and Embryology Department, School of MedicineUdelaRMontevideoUruguay

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