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Mechanism of GA-mediated leaf sheath growth in rice: a proteomic approach

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Gibberellins (GAs) mediated elongation growth and its associated proteomics in rice is relevant to crop productivity in many diversified ecological conditions. There is dearth of precise investigations conducted on proteome dynamics involved in leaf sheath elongation in both wild and mutant rice plants in response to GAs treatments. To explore the molecular mechanism of GA-induced leaf sheath elongation in rice, we conducted a comparative proteomics analysis of wild type rice and a GA biosynthesis deficient mutant osko2. The mutant displayed an obvious dwarfism phenotype, which could be dramatically rescued by application of exogenous GA3. The average levels of GAs in the dwarf mutant plants were lower than the wild type of rice. The proteomic analysis showed a total of 6352 proteins that were quantified, among which 688 differentially expressed proteins were identified as GA-responsive. The biological process classification indicated that metabolic process, cellular process and single-organism process related proteins were the dominant protein groups, and the differentially expressed proteins were largely located in chloroplast, cytoplasm, and nucleus and extracellular. Furthermore, these proteins were mainly involved in cell elongation, signal transduction, photosynthesis, and carbohydrate and protein metabolism. These results suggested that proteins involved in cell elongation, signal transduction and carbohydrate metabolism are regulated by GA in rice leaf-sheath growth.

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The authors thank Dr. Ming Li and Dongli He for providing some experimental technical supports. We thank Kang Ji for helping to culture the rice seedlings. This work was supported by the National Natural Science Foundation of China (NSFC) (No. 31671775).

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Correspondence to Pingfang Yang.

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Hu, Z., Damaris, R.N. & Yang, P. Mechanism of GA-mediated leaf sheath growth in rice: a proteomic approach. Plant Growth Regul (2020). https://doi.org/10.1007/s10725-020-00585-5

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  • Gibberellins
  • Leaf sheath growth
  • Proteome
  • Rice
  • Dwarf mutant