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TaPP2AbBʺ-γ, a wheat regulatory subunit of PP2A enhanced abiotic stress tolerance

  • Dan Liu
  • Bo Li
  • Gang Feng
  • Xinguo Mao
  • Ang Li
  • Xiaoping Chang
  • Ruilian JingEmail author
Original paper
  • 24 Downloads

Abstract

Protein dephosphorylation mediated by serine/threonine protein phosphatase 2A (PP2A) plays critical roles in regulation of abiotic stress responses in plants. Here, we report the involvement of a B″ regulatory subunit of PP2A in wheat (Triticum aestivum L.), TaPP2AbB″-γ, in the positive regulation of stress responses. The transcript of TaPP2AbB″-γ was induced in roots of wheat seedlings by salinity, drought, cold stresses and exogenous ABA application. Overexpression of TaPP2AbB″-γ enhanced the tolerance to various environmental stresses in transgenic Arabidopsis seedlings. Yeast two-hybrid assay demonstrated that TaPP2AbB″-γ interact with the first 100 amino acids of TaBZR1, a positive regulator of Brassinosteroids (BRs) signaling. The expression of TaBZR1 gene was up-regulated in roots upon exposure to NaCl stress. All our results suggested that TaPP2AbB″-γ functions in improving abiotic stress tolerance by directly binding to TaBZR1, pointing to a possible cross-talk of BR signaling with TaPP2AbB″-γ mediated stress response.

Keywords

TaPP2AbBʺ-γ TaBZR1 PP2A BR signaling Stress tolerance 

Notes

Acknowledgements

This work was supported by the National Key R&D Program of China (2016YFD0100605, 2017YFD0300202).

Author contributions

RJ and DL conceived and designed the experiments. DL and BL performed the most experiments and analyzed the data. GF performed the yeast two-hybrid assays. XM and AL performed the experiments of protoplast transfection and fluorescence microscopy analyses. XC prepared the plant materials. DL and RJ contributed to the writing and revising of the manuscript. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests. The funding bodies had no role in study design, data collection, analyses and interpretation, decision to publish or preparation of manuscript.

Supplementary material

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Dan Liu
    • 1
    • 2
  • Bo Li
    • 1
  • Gang Feng
    • 2
  • Xinguo Mao
    • 1
  • Ang Li
    • 1
  • Xiaoping Chang
    • 1
  • Ruilian Jing
    • 1
    Email author
  1. 1.National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop ScienceChinese Academy of Agricultural SciencesBeijingChina
  2. 2.Tianjin Key Laboratory of Crop Genetics and Breeding, Tianjin Crop Research InstituteTianjin Academy of Agricultural SciencesTianjinChina

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