TaPP2AbBʺ-γ, a wheat regulatory subunit of PP2A enhanced abiotic stress tolerance
- 24 Downloads
Protein dephosphorylation mediated by serine/threonine protein phosphatase 2A (PP2A) plays critical roles in regulation of abiotic stress responses in plants. Here, we report the involvement of a B″ regulatory subunit of PP2A in wheat (Triticum aestivum L.), TaPP2AbB″-γ, in the positive regulation of stress responses. The transcript of TaPP2AbB″-γ was induced in roots of wheat seedlings by salinity, drought, cold stresses and exogenous ABA application. Overexpression of TaPP2AbB″-γ enhanced the tolerance to various environmental stresses in transgenic Arabidopsis seedlings. Yeast two-hybrid assay demonstrated that TaPP2AbB″-γ interact with the first 100 amino acids of TaBZR1, a positive regulator of Brassinosteroids (BRs) signaling. The expression of TaBZR1 gene was up-regulated in roots upon exposure to NaCl stress. All our results suggested that TaPP2AbB″-γ functions in improving abiotic stress tolerance by directly binding to TaBZR1, pointing to a possible cross-talk of BR signaling with TaPP2AbB″-γ mediated stress response.
KeywordsTaPP2AbBʺ-γ TaBZR1 PP2A BR signaling Stress tolerance
This work was supported by the National Key R&D Program of China (2016YFD0100605, 2017YFD0300202).
RJ and DL conceived and designed the experiments. DL and BL performed the most experiments and analyzed the data. GF performed the yeast two-hybrid assays. XM and AL performed the experiments of protoplast transfection and fluorescence microscopy analyses. XC prepared the plant materials. DL and RJ contributed to the writing and revising of the manuscript. All authors read and approved the final manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no competing interests. The funding bodies had no role in study design, data collection, analyses and interpretation, decision to publish or preparation of manuscript.
- Eichhorn PJ, Creyghton MP, Bernards R (2009) Protein phosphatase 2A regulatory subunits and cancer. Biochim Biophys Acta 1795:1–15Google Scholar
- Lechward K, Awotunde OS, Swiatek W, Muszyńska G (2001) Protein phosphatase 2A: variety of forms and diversity of functions. Acta Biochim Pol 48:921–933Google Scholar
- Liu SH, Wang CX, Mao XG, Liu HM, Li A, Jing RL (2010) Cloning of protein phosphatase 2A regulatory subunit gene TaBβ-1 and its expression patterns under abiotic stresses in wheat. Sci Agric Sin 385:778–792Google Scholar
- Mujeeb-Kazi A, Leon JLDD (2002) Conventional and alien genetic diversity for salt tolerant wheats: focus on current status and new germplasm development. Springer, AmsterdamGoogle Scholar
- Pang X, Mao X, Jing R, Shi J, Gao T, Chang X, Li Y (2007) Analysis of gene expression profile responsed to water stress in wheat Triticum aestivum L. seedling. Acta Agron Sin 32:333–336Google Scholar
- Tang W, Yuan M, Wang R, Yang Y, Wang C, Oses-Prieto JA, Kim TW, Zhou HW, Deng Z, Gampala SS, Gendron JM, Jonassen EM, Lillo C, DeLong A, Burlingame AL, Sun Y, Wang ZY (2011) PP2A activates brassinosteroid-responsive gene expression and plant growth by dephosphorylating BZR1. Nat Cell Biol 13:124–131CrossRefGoogle Scholar