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Overexpression of MADS-box transcription factor OsMADS25 enhances salt stress tolerance in Rice and Arabidopsis

  • Junyu Wu
  • Chunyan Yu
  • Linli Hunag
  • Minjie Wu
  • Bohan Liu
  • Yihua Liu
  • Ge Song
  • Dongdong Liu
  • Yinbo GanEmail author
Original paper
  • 8 Downloads

Abstract

Salinity is the major abiotic stress inhibiting plant growth and yield all over the world. MADS-box family genes play vital roles in various aspects of plant growth and development, such as the development of floral organs and the regulation of flowering time. The MADS-box transcription factor OsMADS25, belongs to ANR1 clade, and is significantly induced by NO3 and plays a key role in root development in rice. Although MADS genes in different species are reported to play important role in salt stress response, whether OsMADS25 is involved in salinity is still unknown. In this study, our results showed that overexpression of OsMADS25 in rice and Arabidopsis improved salinity tolerance in comparison to wild type. The higher rate of free proline content, lower accumulation of MDA and significantly up-regulation of genes related to salt stress in OsMADS25 overexpression lines clearly demonstrated more tolerance of OsMADS25 plants against oxidative damages to salt stress than WT plants. In contrast, the OsMADS25 RNAi plants showed more sensitivity to salt stress. Overall, our findings indicate OsMADS25 gene is involved in salt tolerance response and this gene could be used as a gene source for salt tolerant crop breeding in future.

Keywords

Salinity Rice Arabidopsis OsMADS25 Enhance salt tolerance 

Notes

Acknowledgements

The research was supported by Major State Basic Research Development Program the National Key R & D Program of China (2016YFD0100701); the National Natural Science Foundation of China (Grant Nos. 31529001; 31570183; 31661143004; 31700524) and Natural Science Foundation of Shandong Province of China (Grant No. ZR2016CB48).

Author contributions

Wu JY and Yu CY contributed to the work equally and should be regarded as co-first authors. Gan YB, Yu CY, Liu BH and Wu MJ designed the experiment. Wu JY and Yu CY prepared the draft of the manuscript. Wu JY, Yu CY, Huang LL, Song G, Liu DD and Liu YH performed the experiments and analyzed the data. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10725_2019_539_MOESM1_ESM.docx (781 kb)
Supplementary material 1 (DOCX 781 kb)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Zhejiang Key Lab of Crop Germplasm, Department of Agronomy, College of Agriculture and BiotechnologyZhejiang UniversityHangzhouChina
  2. 2.College of AgricultureLudong UniversityYantaiChina

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