UV-B response is modulated by cell-type specific signaling pathway in multicellular green algae Volvox carteri
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A fundamental question in biology is how multicellular organisms regulate cellular and physiological processes in response to environmental signals in a tissue/cell type-specific manner. Light is one such cue but little is known about its effect on molecular mechanisms underlying cell-type specific signaling. The Volvox genus presents a Germ-Soma differentiation that can be used to understand the genetic mechanisms of evolutionary transition from single-cell to multicellular organisms. Here we report the transcriptional analysis throughout both asexual and sexual life cycles of Volvox carteri in two different cell types under UV-B light irradiation. Our data show that VcUVR8-V1, the main splice variant of the VcUVR8 transcript, accumulates during initiation of cleavage division. Moreover, the transcript level of VcUVR8-V1 increases in response to the sex inducer. VcUVR8 expression seems to remain the same in both cell types, while VcCOP1, the interacting partner of VcUVR8, is expressed in a cell-type specific manner. Interestingly, illumination with low doses of UV-B leads to an increase of VcCOP1 transcript levels only in the somatic cells. Our results indicate that UV-B signaling pathway is differentially regulated between two cell types and environmental UV-B could be involved in cell-type specific regulation of developmental and physiological processes.
KeywordsUV-B photoreceptor UVR8 COP1 Gene expression Green algae Volvox carteri
This work has been supported by the Center for International Scientific Studies & Collaboration [CISSC (2013)] to JR and DFG Grant KI 1779/1-1 to AK. We would like thank Magdalena Dombrowa and Saskia Wöllner for their great experimental support during this study. Also, we thank Stefania Viola, Wenteng Xu and Girish Beedessee for reading of the manuscript and helpful suggestions.
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Conflict of interest
The authors declare that they have no conflict of interest.
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