Comparative identification, characterization, and expression analysis of bZIP gene family members in watermelon and melon genomes
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The family of basic leucine zipper (bZIP) transcription factors plays diverse crucial roles in numerous biological processes. Despite the identification of bZIP genes in several plants, to our knowledge, bZIP members in watermelon and melon are yet to be comprehensively investigated. The genomes of watermelon and melon encode 59 ClabZIP and 75 CmbZIP putative genes, respectively. Both bZIP protein family members were phylogenetically grouped into seven subfamilies. The majority of bZIP genes in the same subfamily shared similar gene structures and conserved motifs. Chromosome distribution and genetic analysis revealed that 21 duplication events between ClabZIP genes and 106 duplication events between CmbZIP genes have occurred. Further, the three-dimensional structure and functional annotation of bZIP proteins was predicted. For evaluating the expression patterns of ClabZIP and CmbZIP genes, RNA-seq data available in public databases were analyzed. The expression profiles of selected ClabZIP and CmbZIP genes in root and leaf tissues of drought-stressed watermelon and melon were also examined using qRT-PCR. ClabZIP-57, CmbZIP-52, and CmbZIP-31 genes exhibited the highest expression levels after stress exposure in leaf and root tissues. Gene identification studies like the present study offer new perspectives in the analysis of bZIP protein family members and their functions in plants.
KeywordsCucumis melo Citrullus lanatus bZIP transcription factor genes Bioinformatics analysis Drought stress Gene expression analysis
YCA and MCB conceived the study. FC, NMU and YK performed the experiments and carried out the analysis. YCA and MCB wrote the manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare no competing interests.
- Baloglu MC (2014) Genome-wide in silico identification and comparison of Growth Regulating Factor (GRF) genes in Cucurbitaceae family. Plant Omics 7:260–270Google Scholar
- Food and Agriculture Organization of the United Nations (FAO) (2014) FAOstat, statistical databases. Last updated 15 Aug 2014. http://www.fao.org
- Huang X-S, Liu J-H, Chen X-J (2010) Overexpression of PtrABF gene, a bZIP transcription factor isolated from Poncirus trifoliata, enhances dehydration and drought tolerance in tobacco via scavenging ROS and modulating expression of stress-responsive genes. BMC Plant Biol 10:230–230. https://doi.org/10.1186/1471-2229-10-230 CrossRefGoogle Scholar
- Kavas M, Baloglu MC, Atabay ES, Ziplar UT, Dasgan HY, Unver T (2016) Genome-wide characterization and expression analysis of common bean bHLH transcription factors in response to excess salt concentration. Mol Genet Genomics 291:129–143. https://doi.org/10.1007/s00438-015-1095-6 CrossRefGoogle Scholar
- Lara P, Oñate-Sánchez L, Abraham Z, Ferrándiz C, Díaz I, Carbonero P, Vicente-Carbajosa J (2003) Synergistic activation of seed storage protein gene expression in Arabidopsis by ABI3 and two bZIPs related to OPAQUE2. J Biol Chem 278:21003–21011. https://doi.org/10.1074/jbc.M210538200 CrossRefGoogle Scholar
- Lucas TJ, Lucas WJ (2006) Integrative plant biology: role of phloem long-distance macromolecular trafficking annual. Rev Plant Biol 57:203–232. https://doi.org/10.1146/annurev.arplant.56.032604.144145 CrossRefGoogle Scholar
- Silveira AB, Gauer L, Tomaz JP, Cardoso PR, Carmello-Guerreiro S, Vincentz M (2007) The Arabidopsis AtbZIP9 protein fused to the VP16 transcriptional activation domain alters leaf and vascular development. Plant Sci 172:1148–1156. https://doi.org/10.1016/j.plantsci.2007.03.003 CrossRefGoogle Scholar
- Wang J, Zhou J, Zhang B, Vanitha J, Ramachandran S, Jiang S-Y (2011) Genome-wide expansion and expression divergence of the basic leucine zipper transcription factors in higher plants with an emphasis on SorghumF. J Integr Plant Biol 53:212–231. https://doi.org/10.1111/j.1744-7909.2010.01017.x CrossRefGoogle Scholar
- Yoshida T et al (2010) AREB1, AREB2, and ABF3 are master transcription factors that cooperatively regulate ABRE-dependent ABA signaling involved in drought stress tolerance and require ABA for full activation. Plant J 61:672–685. https://doi.org/10.1111/j.1365-313X.2009.04092.x CrossRefGoogle Scholar