Histone acetylation modification affects cell wall degradation and aerenchyma formation in wheat seminal roots under waterlogging
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Recent studies have shown that histone acetylation, which is an important epigenetic modification, plays a key role in abiotic stress responses in plants. Programmed cell death and aerenchyma formation occur in cortical cells of wheat seminal roots under waterlogging stress. To explore the role of histone acetylation in aerenchyma formation of cortical cells under waterlogging, the seminal roots of two wheat cultivars, namely, Huamai 8 (waterlogging-tolerant) and Huamai 9 (waterlogging-sensitive) were investigated with waterlogging and simultaneous treatment with an acetylation inhibitor. In this study, the immunefluorescence technique and Western blotting were used to determine the histone acetylation levels in wheat seminal roots under waterlogging stress. Cell wall degradation-related enzymes (cellulase and pectinase) were observed using the method of ultracytochemical localization. We also tested the expression of related genes, such as histone acetyltransferase (HAT1), histone deacetylase (HD3), endoglucanase (CEL), polygalacturonase (PG), and xyloglucan endotransglucosylase (XET). The results indicated that histone acetylation is involved in eliciting responses to waterlogging stress as well as in the aerenchyma formation by affecting cell wall degradation of cortical cells in wheat seminal roots. We also present a model of waterlogging-induced aerenchyma formation in cortical cells of wheat seminal roots based on our experimental results and the findings of previous studies.
KeywordsWheat (Triticum aestivum L.) Waterlogging stress Histone acetylation Aerenchyma formation Cell wall degradation Ultracytochemical localization
Polyacrylamide gel electrophoresis
Programmed cell death
Polymerase chain reaction
Sodium dodecyl sulfate
Transmission electron microscopy
This work was supported by the National Nature Science Foundation of China (Grant Nos. 31071347 and 31471428). We would like to thank Cao Jianbo and He Limin for providing the transmission electron microscope technology and the LetPub for providing linguistic assistance during the preparation of this manuscript.
LC conceived and designed research. LC and LD conducted experiments. GB contributed new reagents or analytical tools. LD, YL and LZ analyzed data. LC wrote the manuscript under the help of DX and ZZ. All authors read and approved the manuscript.
Compliance with ethical standards
Conflicts of interest
We declare that the authors have no conflict of interest.
- Dai Y, Ni Z, Dai J, Zhao T, Sun Q (2005) Isolation and expression analysis of genes encoding DNA methyltransferase in wheat (Triticum aestivum L.). Biochem Biophys Acta 1729:118–125Google Scholar
- Gunawardena AHLAN, Pearce DME, Jackson MB, Hawes CR, Evans DE (2001b) Rapid changes in cell wall pectic polysaccharides are closely associated with early stages of aerenchyma formation, a spatially localized form of programmed cell death in roots of maize (Zea mays L.) promoted by ethylene. Plant Cell Environ 24:1369–1375CrossRefGoogle Scholar
- Kong Y, Wang Z, Gu YJ, Xiong F, Chen G, Han Y (2009) Ethylene-induced aerenchyma formation of root in rice. Chin J Rice Sci 23:65–70Google Scholar
- Li C, Xu J, Li J, Li Q, Yang H (2014) Involvement of Arabidopsis histone acetyltransferase HAC family genes in the ethylene signaling pathway. Plant Signal Behav 55:426Google Scholar
- Xu WL, Han M, Yuan ZL (2017) Effects of 3 triterpenoids on bioactivity and cellulase activity of Reticulitermes speratus. Shandong Agric Sci 49(11):98–104Google Scholar
- Zhang F, Yue TL, Fei J, Yuan YH, Gao ZP (2004) Research on measuring method of PG activity. Acta Agric Boreali-occidentalis Sinica 13(4):134–137Google Scholar