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Plant Growth Regulation

, Volume 87, Issue 1, pp 149–163 | Cite as

Histone acetylation modification affects cell wall degradation and aerenchyma formation in wheat seminal roots under waterlogging

  • Chengyang Li
  • Dongcheng Liu
  • Ze Lin
  • Bin Guan
  • Dan Liu
  • Li Yang
  • Xiangyi Deng
  • Fangzhu Mei
  • Zhuqing ZhouEmail author
Original paper
  • 64 Downloads

Abstract

Recent studies have shown that histone acetylation, which is an important epigenetic modification, plays a key role in abiotic stress responses in plants. Programmed cell death and aerenchyma formation occur in cortical cells of wheat seminal roots under waterlogging stress. To explore the role of histone acetylation in aerenchyma formation of cortical cells under waterlogging, the seminal roots of two wheat cultivars, namely, Huamai 8 (waterlogging-tolerant) and Huamai 9 (waterlogging-sensitive) were investigated with waterlogging and simultaneous treatment with an acetylation inhibitor. In this study, the immunefluorescence technique and Western blotting were used to determine the histone acetylation levels in wheat seminal roots under waterlogging stress. Cell wall degradation-related enzymes (cellulase and pectinase) were observed using the method of ultracytochemical localization. We also tested the expression of related genes, such as histone acetyltransferase (HAT1), histone deacetylase (HD3), endoglucanase (CEL), polygalacturonase (PG), and xyloglucan endotransglucosylase (XET). The results indicated that histone acetylation is involved in eliciting responses to waterlogging stress as well as in the aerenchyma formation by affecting cell wall degradation of cortical cells in wheat seminal roots. We also present a model of waterlogging-induced aerenchyma formation in cortical cells of wheat seminal roots based on our experimental results and the findings of previous studies.

Keywords

Wheat (Triticum aestivum L.) Waterlogging stress Histone acetylation Aerenchyma formation Cell wall degradation Ultracytochemical localization 

Abbreviations

CCD

Charge-coupled device

CEL

Endoglucanase cellulase

HAT

Histone acetyltransferase

HDAC

Histone deacetylase

PAGE

Polyacrylamide gel electrophoresis

PCD

Programmed cell death

PCR

Polymerase chain reaction

PG

Polygalacturonase pectinase

SDS

Sodium dodecyl sulfate

XET

Xyloglucan endotransglycosylase

TEM

Transmission electron microscopy

Notes

Acknowledgements

This work was supported by the National Nature Science Foundation of China (Grant Nos. 31071347 and 31471428). We would like to thank Cao Jianbo and He Limin for providing the transmission electron microscope technology and the LetPub for providing linguistic assistance during the preparation of this manuscript.

Author contributions

LC conceived and designed research. LC and LD conducted experiments. GB contributed new reagents or analytical tools. LD, YL and LZ analyzed data. LC wrote the manuscript under the help of DX and ZZ. All authors read and approved the manuscript.

Compliance with ethical standards

Conflicts of interest

We declare that the authors have no conflict of interest.

Supplementary material

10725_2018_460_MOESM1_ESM.docx (2.6 mb)
Supplementary material 1 (DOCX 2666 KB)

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Chengyang Li
    • 1
  • Dongcheng Liu
    • 1
  • Ze Lin
    • 1
  • Bin Guan
    • 1
  • Dan Liu
    • 1
  • Li Yang
    • 1
  • Xiangyi Deng
    • 2
  • Fangzhu Mei
    • 3
  • Zhuqing Zhou
    • 1
    Email author
  1. 1.Laboratory of Cell Biology, College of Life Science and TechnologyHuazhong Agricultural UniversityWuhanChina
  2. 2.College of Food & Biological Science and TechnologyWuhan Institute of Design and SciencesWuhanChina
  3. 3.College of Plant Sciences & TechnologyHuazhong Agricultural UniversityWuhanChina

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