Regulation of soybean SUMOylation system in response to Phytophthora sojae infection and heat shock
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Modification of protein substrates by small ubiquitin-related modifier (SUMO) plays a vital role in plants under biotic and abiotic stresses. However, its role in the stress responses of soybean (Glycine max (L.) Merrill) is poorly understood. In this study, we explored SUMOylation in soybean in response to Phytophthora sojae race 1 infection and heat shock. We selected two soybean cultivars for these analyses; one resistant to P. sojae race 1 (SN10), and one susceptible (HF25). The transcription and expression levels of SUMOylation-related genes were detected in SN10 and HF25 after inoculation with P. sojae race 1 and after exposure to heat shock (42 °C). After inoculation with P. sojae race 1, GmSUMO2/3 and GmSAE1b accumulated in the roots. After the heat shock treatment, GmSCEd and GmE3f accumulated in the leaves. The transcript levels of GmESD4d increased in response to both P. sojae infection and heat shock. We monitored SUMOylation in the root, stem, and leaves after the stress treatments. We detected SUMO conjugates in the unifoliolate leaf, trifoliolate leaf, root, and stem after the heat shock treatment. SUMO conjugates accumulated mainly in the root and stem in response to heat shock, but mainly in the root in response to P. sojae race 1 infection. The accumulation of SUMO conjugates in response to stress indicated that SUMOylation enhanced the resistance of soybean to P. sojae and heat shock. These results provide a foundation for further research on the role of SUMOylation in resistance to P. sojae and heat shock.
KeywordsSoybean SUMOylation Phytophthora sojae Heat shock
Small ubiquitin-like modifier
- SAE SUMO
- SCE SUMO
Quantitative real time PCR
Relative water content
This study was financially supported by the National Natural Science Foundation of China (Grant No. 31501332), and the National Key Research & Development Program of China (Grant No. 2016YFD0100500, 2016YFD0100300 and 2016YFD0100201-21). We thank Jennifer Smith, PhD, from Liwen Bianji, Edanz Group China (http://www.liwenbianji.cn/ac), for editing the English text of a draft of this manuscript.
QC, CL, and DX designed the study. SL analyzed the data and wrote the paper. ML, JW, LZ, ML, ZH, ZQ, HJ, and YF participated in correcting the manuscript. All authors have read and approved the final manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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