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Plant Growth Regulation

, Volume 87, Issue 1, pp 19–27 | Cite as

Functional analysis for domains of maize PPR protein EMP5 in RNA editing and plant development in Arabidopsis

  • Peng Zheng
  • Qiang He
  • Xiaomin Wang
  • Jumin Tu
  • Jianhua Zhang
  • Yu-Jun LiuEmail author
Original paper
  • 51 Downloads

Abstract

EMP5 and OTP72 are PPR proteins involved in mitochondrial same site RNA editing of rpl16 in maize and Arabidopsis, respectively. Besides rpl16, EMP5 also affects the editing efficiency of some editing sites in other mitochondrial transcripts. Loss of function of EMP5 causes arrested embryogenesis and endosperm development in maize, but no discernable defective phenotype is observed in Arabidopsis otp72 mutant plants. We here report that EMP5 can completely complement Arabidopsis otp72 for Ararpl16-440 editing, and E domain can’t be disrupted for normal function of EMP5. An interesting finding was that overexpression of a set of truncated EMP5s in Col-0 resulted in delayed bolting phenotypes and smaller plants. It seems some truncated EMP5s, such as EMP5-C and EMP5-D, have yet unknown functions, are not responsible for Ararpl16-440 editing any more. The phylogenetic analysis indicated that the functions of most EMP5 orthologs may be conserved, but EMP5 ortholog proteins from Nicotiana and Solanum can’t be found, and the C to U editing of Zmrpl16-458 site was not needed any more in the two species. Therefore, these two primary orthologs maybe already evolve new functions and change to different new proteins during evolutionary history. Overall, our studies revealed that E domain is crucial for mitochondrial RNA editing function of EMP5, and a set of truncated EMP5s still have functions for Arabidopsis normal growth and development, providing new insights into investigating the functions of EMP5 protein.

Keywords

PPR proteins E domain RNA editing Maize Arabidopsis 

Notes

Acknowledgements

We thank Tsuyoshi Nakagawa (Shimane University, Japan) for the pGWB vectors. This work was supported by funds from the National Natural Science Foundation of China (Project No. 31501375) and China Postdoctoral Science Foundation Funded Project (Project No. 2016M590542).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10725_2018_447_MOESM1_ESM.pdf (2.1 mb)
Supplementary material 1 (PDF 2113 KB)

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Peng Zheng
    • 1
  • Qiang He
    • 3
  • Xiaomin Wang
    • 3
  • Jumin Tu
    • 1
  • Jianhua Zhang
    • 2
  • Yu-Jun Liu
    • 1
    Email author
  1. 1.Institute of Crop Science, College of Agriculture and BiotechnologyZhejiang UniversityHangzhouPeople’s Republic of China
  2. 2.State Key Lab of Agrobiotechnology, School of Life ScienceThe Chinese University of Hong KongShatin, New TerritoriesPeople’s Republic of China
  3. 3.School of Life SciencesLanzhou UniversityLanzhouPeople’s Republic of China

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