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Transcriptome profiles reveal new regulatory factors of anthocyanin accumulation in a novel purple-colored cherry tomato cultivar Jinling Moyu

  • Yinlei Wang
  • Zhidan Luo
  • Chen Lu
  • Rong Zhou
  • Huiming Zhang
  • Liping Zhao
  • Wengui Yu
  • TongMin Zhao
Original paper
  • 73 Downloads

Abstract

Anthocyanins are important secondary metabolites with high nutraceutical value. They represent the major red, purple, violet and blue pigments in higher plants but are usually not present in the fruits of domesticated tomatoes. Recently, several genes that regulate anthocyanin accumulation have been identified in wild tomatoes or mutation lines; however, whether there are other new factors involved in the regulation of anthocyanin biosynthesis is still unknown. “Jinling Moyu” (MY) is a novel purple cherry tomato that accumulates considerable anthocyanins in its ripe fruit. A transcriptome comparison analysis of MY fruits with pink and green fruit revealed that several structural genes assigned to the anthocyanin biosynthetic pathway were highly expressed in MY tomato, but all six known regulatory genes of anthocyanin accumulation in tomato fruits were rarely expressed among the samples in this study. However, four other R2R3–MYB and five other bHLH transcription factors were highly correlated with the differentially expressed structural genes in the anthocyanin pathway, indicating that these transcription factors might regulate anthocyanin biosynthesis in MY tomato. Our study will aid in understanding the complex mechanism regulating anthocyanin pigmentation in tomato fruits .

Keywords

Tomato Purple fruit Anthocyanin Transcription factor 

Notes

Acknowledgements

We thank Drs. Jinhua Li and Yong Xue for their critical review of this manuscript. We also thank Springer-Nature Author Service for English Language Editing. This work was financially supported by the National Key Research and Development Plan [Grant No. 2016YFD0101703], the National Natural Science Foundation of China (Grant No. 31501763 and 31501022), Natural Science Foundation of Jiangsu Province, China (Grant No. BK20140440 and BK20140443) and the Jiangsu Agricultural Independent Innovation Foundation of China [Grant No. CX(14)5084].

Author contributions

YW designed the research, collected the samples and performed the anthocyanin extraction and quantification. ZL collected the samples, and performed the RNA isolation and qRT-PCR experiments. CL analyzed the RNA-Seq data and helped in manuscript writing. RZ and LZ helped in collecting the samples. HZ helped in data analysis. WY contributed to concept the idea. TZ designed the research and wrote the manuscript. All authors have read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interest.

Supplementary material

10725_2018_444_MOESM1_ESM.pdf (320 kb)
Supplementary material 1 (PDF 319 KB)

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Institute of Vegetable CropsJiangsu Academy of Agricultural SciencesNanjingChina
  2. 2.Jiangsu Key Laboratory for Horticultural Crop Genetic ImprovementNanjingChina
  3. 3.Lianyungang Institute of Agricultural Sciences in Jiangsu Xuhuai AreaJiangsu Academy of Agricultural SciencesLianyungangChina
  4. 4.Jiangsu Key Laboratory of Marine Pharmaceutical Compound ScreeningHuaihai Institute of TechnologyLianyungangChina
  5. 5.Co-Innovation Center of Jiangsu Marine Bio-industry TechnologyHuaihai Institute of TechnologyLianyungangChina
  6. 6.Jiangsu Marine Resources Development Research InstituteLianyungangChina
  7. 7.Jiangsu Yugong Biolabs Inc.LianyungangChina

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