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Plant Growth Regulation

, Volume 86, Issue 2, pp 211–222 | Cite as

Characterization of the glutathione S-transferase (GST) gene family in Pyrus bretschneideri and their expression pattern upon superficial scald development

  • Libin Wang
  • Ming Qian
  • Runze Wang
  • Li Wang
  • Shaoling Zhang
Original paper

Abstract

Glutathione S-transferases (GSTs) proposedly play a crucial role in the development of superficial scald in pear fruit; however, the specific member from pear GST gene family, which plays an important role in this process, has not been identified until recently. In this study, a total of 62 GST family genes were identified in Pyrus bretschneideri genome, which were distributed across the 14 chromosomes and 8 scaffolds with an uneven distribution. They could be categorized into eight classes based on phylogenetic analysis, and WGD/segmental duplication mainly drove their expansion. The expression of PbrGSTs in ‘Yali’ pear fruit was tissue-specific. In accompany with superficial scald development was the accumulation of reactive oxygen species (ROS) accumulation and loss of ascorbic acid (AsA) in the pericarp of ‘Yali’ pear. Of 47 PbrGSTs detected in the pericarp, the transcription of 25 members were enhanced upon the development of superficial scald, while three in dehydroascorbate reductase (DHAR) class were downregulated in association with lower DHAR activity. In combination with the results of the impact of 1-MCP and MHO fumigation on the expression profile of PbrGSTs, PbrDHAR1, PbrDHAR2 and PbrDHAR4 were selected as the candidate gene involved in superficial scald development.

Keywords

Evolution GST Low temperature stress Pear Physiological disorder 

Notes

Acknowledgements

We would like to thank the financial support to this study from the National Natural Science Foundation of China (31701868), China Postdoctoral Science Foundation (2017M620213 and 2017M621760), and the Fundamental Research Funds for the Central Universities (KJQN201813). Additionally, we thank Weiqi Luo (Horticultural Research Laboratory, Fort Pierce, Florida, USA) for providing some support with the statistical analysis.

Compliance with ethical standards

Conflict of interest

There has no conflict of interest among authors.

Supplementary material

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Centre of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm EnhancementNanjing Agricultural UniversityNanjingChina

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