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Plant Growth Regulation

, Volume 86, Issue 2, pp 181–194 | Cite as

Exogenously applied salicylic acid maintains redox homeostasis in salt-stressed Arabidopsis gr1 mutants expressing cytosolic roGFP1

  • Jolán Csiszár
  • Szilvia Brunner
  • Edit Horváth
  • Krisztina Bela
  • Petra Ködmön
  • Riyazuddin Riyazuddin
  • Ágnes Gallé
  • Ágnes Hurton
  • Csaba Papdi
  • László Szabados
  • Irma Tari
Original paper
  • 241 Downloads

Abstract

Exogenous salicylic acid (SA) can be used for chemical hardening to alleviate oxidative stress in plants exposed to salinity. The treatment of 5-week-old Arabidopsis thaliana plants with increasing doses of SA alters the ascorbate (ASC) and glutathione (GSH) pools, and modulates their redox status and the activity of several antioxidant enzymes, such as ascorbate peroxidase (APX) and glutathione reductase (GR). To investigate the role of GR in the maintenance of cytoplasmic redox homeostasis after hardening by SA, wild type (WT) and gr1 mutant plants, expressing the cytoplasmic redox-sensitive green fluorescent protein (c-roGFP1), were pre-treated with 10−7 and 10−5 M SA for 2 weeks and subsequently exposed to 100 mM NaCl. The redox status of the salt-stressed WT plants became more oxidized, which was prevented by pretreatment with 10−5 M SA. The gr1 mutants showed more positive redox potential than WT plants, which could be reversed by treatment with 10−5 M SA. In mutants, the increased GSH levels may have compensated for the deleterious effect of GR deficiency and stabilized the redox potential in plants exposed to salinity. The ASC regeneration in WT plants shifted from the GSH-dependent dehydroascorbate reductase (DHAR) reaction to the NAD(P)H-dependent monodehydroascorbate reductase (MDHAR) activity during chemical hardening, which contributed to the preservation of the GSH pool in plants under salt stress. Our results suggest that the maintenance of GSH levels and redox homeostasis by SA-mediated hardening play a major role in priming and defending against salt stress.

Keywords

Ascorbate–glutathione pool Glutathione reductase Redox homeostasis Redox-sensitive GFP1 Salt stress 

Abbreviations

APX

Ascorbate peroxidase

ASC

Ascorbate

c-roGFP1

Cytoplasmic redox-sensitive green fluorescent protein

DHAR

Dehydroascorbate reductase

EGSH

Glutathione reduction potential

EroGFP

Reduction potential of roGFP1

GR

Glutathione reductase

gr1

Glutathione reductase1 mutant

GSH

Reduced glutathione

GSSG

Glutathione disulphide, oxidized glutathione

MDHAR

Monodehydroascorbate reductase

NPR1

Non-expressor of pathogenesis-related genes1

ROS

Reactive oxygen species

SA

Salicylic acid

TRX

Thioredoxin

WT

Wild type

Notes

Acknowledgements

We would like to thank Dr. M. Schwarzländer for the c-roGFP1-harbouring Arabidopsis seeds. This study was supported by the Hungarian National Research, Development and Innovation Office [Grant Numbers: OTKA K 105956 and NKFI-1 PD 121027] and by the Hungary-Serbia IPA Cross-border Co-operation Programme [HUSRB/1203/221/173].

Supplementary material

10725_2018_420_MOESM1_ESM.docx (16 kb)
Supplementary material 1 (DOCX 15 KB)

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Jolán Csiszár
    • 1
  • Szilvia Brunner
    • 1
  • Edit Horváth
    • 1
  • Krisztina Bela
    • 1
  • Petra Ködmön
    • 1
  • Riyazuddin Riyazuddin
    • 1
  • Ágnes Gallé
    • 1
  • Ágnes Hurton
    • 1
  • Csaba Papdi
    • 2
  • László Szabados
    • 2
  • Irma Tari
    • 1
  1. 1.Department of Plant Biology, Faculty of SciencesUniversity of SzegedSzegedHungary
  2. 2.Institute of Plant BiologyBiological Research Centre of HASSzegedHungary

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