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Plant Growth Regulation

, Volume 86, Issue 2, pp 169–180 | Cite as

PpVIN2, an acid invertase gene family member, is sensitive to chilling temperature and affects sucrose metabolism in postharvest peach fruit

  • Xingxing He
  • Yingying Wei
  • Jingyu Kou
  • Feng Xu
  • Zhonghua Chen
  • Xingfeng Shao
Original paper
  • 77 Downloads

Abstract

We previously reported that expression and activity of acid invertases (AI) are increased in peach fruit under chilling stress. In order to determine which AI genes respond to chilling stress, seven AI genes, two vacuolar invertases (VINs) and five cell wall-bound invertases (CWINs), were identified and cloned. The predicted amino acid sequences of the genes contain conserved sites characteristic of plant AIs such as NDPNG/A, the sucrose-binding site, and MWECV/P, a cysteine catalytic motif. Using gene-specific primers, the expression of each gene was measured in ‘Baifeng’ and ‘Yulu’ peach fruits stored at 0, 5, 10 and 20 °C. Of the seven genes, expression of PpVIN2 was the most affected by chilling stress; the largest increases were in fruit stored at 5 °C, up to 17-fold in ‘Baifeng’ fruit, and up to 280-fold in ‘Yulu’ fruit. Overall, VIN activity was much higher than CWIN activity in stored peach fruit. In both cultivars reducing sugar content increased significantly and sucrose content decreased gradually during storage at 5 °C relative to other temperatures, and was accompanied by severe chilling injury symptoms. Thus, PpVIN2 appears to be induced by chilling and may play an important role in sucrose metabolism in peach fruit subjected to cold storage.

Keywords

Prunus persica Sugar metabolism Acid invertase Gene cloning Gene expression Chilling injury 

Notes

Acknowledgements

This study was sponsored by the National Natural Science Foundation of China (No. 31671903), the Nature Science Foundation of Zhejiang Province (No. LR15C200002), and the K. C. Wong Magna Fund in Ningbo University.

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Department of Food Science and EngineeringNingbo UniversityNingboChina
  2. 2.School of Science and HealthWestern Sydney UniversityPenrithAustralia
  3. 3.China-Australia Joint Center for Food and HealthNingbo University & Western Sydney UniversityNingboChina
  4. 4.Australia-China Joint Center for Food and HealthWestern Sydney University & Ningbo UniversityPenrithAustralia

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