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Plant Growth Regulation

, Volume 86, Issue 2, pp 149–157 | Cite as

Lsi1-regulated Cd uptake and phytohormones accumulation in rice seedlings in presence of Si

  • Hongmei Lin
  • Jianyu He
  • Weiwei Lin
  • Yingzhe Li
  • Changxun Fang
  • Wenxiong Lin
Original paper
  • 116 Downloads

Abstract

Silicon (Si) is known for its role in regulating the response of plants to imposed abiotic stresses. Since the stresses generally hinder production of a crop, such as rice, the exploration of the biochemistry and plant physiology relating to the function is of interest. Indeed, recently, there were reports on the function of Lsi1 in regulating the tolerance of rice to cadmium (Cd) stress. This study compared the kinetics of the Cd uptakes in Lemont wild type rice and its transgenic lines exposed to Cd with or without exogenous Si supply. At the same time, changes on the endogenous phytohormones and growth of the rice seedlings were monitored. Genetically, Lsi1 overexpression was found to downregulate Km and Vmax of Cd uptake kinetics in the plants under Cd stress, especially in the presence of Si. On the other hand, Lsi1 RNAi upregulated Km and Vmax regardless whether Si was present or not. It implied that Lsi1 could be capable of regulating Si as well as Cd transports. Under Cd stress, addition of Si reduced the Cd uptake of the rice lines in the order of Lsi1-overexpression line > Lemont > Lsi1-RNAi line. In addition, it also affected the chlorophyll biosynthesis and dry mass accumulation of the rice plants under Cd stress. Analyses on phytohormones including IAA, GA3, JA, SA and ABA, as well as physiological functions, of the seedlings further verified the active involvement of Lsi1 in the complex defense system of the plants against Cd stress.

Keywords

Rice (Oryza sativaSilicon Cadmium Lsi1 Phytohormone Uptake kinetics 

Abbreviations

Si

Silicon

Cd

Cadmium

Lsi1

Low Si-influx 1

WT

Lemont wild type rice

Lsi1-OE line

Lsi1-overexpression transgenic Lemont rice

Lsi1-RNAi line

Lsi1-RNAi transgenic Lemont rice

Km

Michaelis constant

Vmax

Maximum influx rate

IAA

Indoleacetic acid

GA3

Gibberellin3

JA

Jasmonic acid

SA

Salicylic acid

ABA

Abscisic acid

Notes

Acknowledgements

This research work was supported by the National Natural Science Foundation of China (Nos. 31271670, 31300336), the National Research Foundation for the Doctoral Program of Higher Education of China (No. 20133515130001) and Fujian-Taiwan Joint Innovative Centre for Germplasm Resources and Cultivation of Crops (Fujian 2011 Program, 2015,75). We also acknowledged financial support for the project from Minjiang University.

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Institute of OceanographyMinjiang UniversityFuzhouPeople’s Republic of China
  2. 2.Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life SciencesFujian Agriculture and Forestry UniversityFuzhouPeople’s Republic of China
  3. 3.Key Laboratory of Crop Ecology and Molecular Physiology (Fujian Agriculture and Forestry University)Fujian Province UniversityFuzhouPeople’s Republic of China
  4. 4.Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of CropsFujian Agriculture and Forestry UniversityFuzhouPeople’s Republic of China

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