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Preliminary characterization of floral response of Xerophyta humilis to desiccation, vernalisation, photoperiod and light intensity

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Abstract

Xerophyta humilis is a monocotyledonous resurrection plant found in arid and semi-arid summer rainfall areas of Southern Africa, which undergoes desiccation to survive periods of extreme drought. In order for X.humilis to thrive in their natural habitat, correct timing of the floral transition, coincident with wet periods of sufficient duration, is essential. In this study, the environmental cues involved in the regulation of the floral transition in X.humilis were analysed. No single parameter tested was sufficient to induce flowering, but it was found that flowering was promoted by a combination of a cool period experienced while plants were hydrated, followed by transfer to long-day photoperiods of relatively high light intensity. Plants retained competence to flower if desiccated during exposure to cold, but no flowering occurred if dried prior to this exposure. These data suggest that exposure to cold temperature facilitates vernalisation and subsequent exposure to high light and long days are inductive for floral initiation in X. humilis.

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References

  1. Blázquez MA, Ahn JH, Weigel D (2003) A thermosensory pathway controlling flowering time in Arabidopsis thaliana. Nat Genet 33:168–171

  2. Boss PK, Bastow RM, Mylne JS, Dean C (2004) Multiple pathways in the decision to flower: enabling, promoting, and resetting. Plant Cell 16:S18–S31

  3. Collett H, Butowt R, Smith J, Farrant J, Illing N (2003) Photosynthetic genes are differentially transcribed during the dehydration-rehydration cycle in the resurrection plant, Xerophyta humilis. J Exp Bot 54:2593–2595

  4. Collett H, Shen A, Gardner M, Farrant JM, Denby KJ, Illing N (2004) Towards profiling of desiccation tolerance in Xerophyta humilis (Bak.) Dur and Schinz: construction of a normalized 11 k X. humilis cDNA set and microarray expression analysis of 424 cDNAs in response to dehydration. Physiol Plant 122:39–53

  5. Cooper K, Farrant JM (2002) Recovery of the resurrection plant Craterostigma wilmsii from desiccation: protection vs. repair. J Exp Bot 53:1805–1813

  6. Dace H, Sherwin HW, Illing N, Farrant JM (1998) Use of metabolic inhibitors to elucidate mechanisms of recovery from desiccation stress in the resurrection plant Xerophyta humilis. Plant Growth Regul 24:171–177

  7. Ensminger I, Busch F, Huner NPA (2006) Photostasis and cold acclimation: sensing low temperature through photosynthesis. Physiol Plant 126:28–44

  8. Farrant JM (2007) Mechanisms of desiccation tolerance in angiosperm resurrection plants. In: Jenks MA, Wood AJ (eds) Plant desiccation tolerance. Wiley, Ames, pp 51–90

  9. Henderson IR, Dean C (2004) Control of Arabidopsis flowering: the chill before the bloom. Development 131:3829–3838

  10. King RW, Dawson IA, Speer SS (2002) Control of growth and flowering in two Western Australian species of Pimelea. Aust J Bot 40:377–388

  11. King RW, Worrall R, Dawson IA (2008) Diversity in environmental controls of flowering in Australian plants. Sci Hortic 118:161–167

  12. Koornneef M, Hanhart CJ, Van der Veen JH (1991) A genetic and physiological analysis of late flowering mutants in Arabidopsis thaliana. Mol Gen Genet 229:57–66

  13. Lang A (1965) Physiology of flower initiation. In: Ruhland W (ed) Encyclopedia of plant physiology. Springer, Berlin, pp 1371–1536

  14. Lin C (2000) Photoreceptors and regulation of flowering time. Plant Physiol 132:39–50

  15. Mouradov A, Cremer F, Coupland G (2002) Control of flowering time: interacting pathways as a basis for diversity. Plant Cell 14(supplement):s111–s130

  16. Napp-Zinn K (1987) Vernalisation. Environmental and genetic regulation. In: Atherton JG (ed) Manipulation of flowering. Butterworths, London, pp 123–132

  17. Sherwin HW, Farrant JM (1996) Differences in rehydration of three desiccation—tolerant angiosperm species. Ann Bot 78:703–710

  18. Sherwin HW, Farrant JM (1998) Protection mechanisms against excess light in the resurrection plants Craterostigma wilmsii and Xerophyta viscosa. Plant Growth Regul 24:203–210

  19. Sung S, Amasino RM (2005) Remembering winter: towards a molecular understanding of vernalization. Annu Rev Plant Biol 56:491–508

  20. Thomas B (2006) Light signals and flowering. J Exp Bot 57:3387–3393

  21. Yeang H-Y (2007) Synchronous flowering of the rubber tree (Hevea brasiliensis) induced by solar radiation intensity. New Phytol 175:283–289

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Acknowledgments

MYM received an NRF bursary; this study was supported by NRF funding to LCR and JMF. We are grateful to Keren Cooper for her assistance with plant maintenance; to Neil Bredekamp and Des Barnes for assistance with the controlled growth facilities.

Author information

Correspondence to Laura C. Roden.

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Myers, M.Y., Farrant, J.M. & Roden, L.C. Preliminary characterization of floral response of Xerophyta humilis to desiccation, vernalisation, photoperiod and light intensity. Plant Growth Regul 62, 213–216 (2010) doi:10.1007/s10725-010-9460-2

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Keywords

  • Xerophyta humilis
  • Flowering
  • Photoperiod
  • Light intensity
  • Vernalisation
  • Cold sensing