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Genetic Resources and Crop Evolution

, Volume 66, Issue 7, pp 1469–1482 | Cite as

Genomic characterization of the Native Seeds/SEARCH common bean (Phaseolus vulgaris L.) collection and its seed coat patterns

  • Di Wu
  • Joy Hought
  • Matheus Baseggio
  • John P. Hart
  • Michael A. Gore
  • Daniel C. IlutEmail author
Research Article

Abstract

Common bean (Phaseolus vulgaris L.) is one of the most important legume crops for human consumption. The Native Seeds/SEARCH common bean collection consists of locally-adapted accessions collected from the southwestern US and northwestern Mexico. In this study, a representative panel of nearly 300 accessions from this collection was genotyped with more than 10,000 high-quality SNP markers and phenotyped for seed coat patterns. The collection consists primarily of accessions from the Mesoamerican gene pool, and they separate into three distinct subpopulations, with strong population differentiation (FST > 0.4) observed between them. Through a genome-wide association study with the Mesoamerican accessions, we identified several SNPs on chromosome 8 that are associated with seed coat pattern traits and reside proximal to the putative location of the C locus, a locus previously shown to control the pattern of the seed coat. Five myb transcription factors linked to these SNPs were identified as candidate causal genes for seed coat patterns controlled by the C locus. Furthermore, we identified a potentially novel locus on chromosome 10 that appears to control the Anasazi seed coat phenotype. Our work is the first to characterize the genetic diversity of the Native Seeds/SEARCH common bean collection, providing valuable genetic information for germplasm conservation efforts.

Keywords

Common bean Genome-wide association study Population genetics Seed coat pattern 

Abbreviations

BIC

Bayesian information criterion

FDR

False discovery rate

FIT

Index of panmixia

FST

Fixation index

GBS

Genotyping-by-sequencing

GWAS

Genome-wide association study

IBS

Identity–by–state

LD

Linkage disequilibrium

MLMM

Multi-locus mixed model

NPGS

National plant germplasm system

NS/S

Native Seeds/SEARCH

PA

Proanthocyanidins

PCA

Principal component analysis

SFS

Site frequency spectrum

SNP

Single-nucleotide polymorphism

Notes

Acknowledgements

This research was supported by Cornell University startup funds (M.A.G.). We thank the Department of Energy Joint Genome Institute and collaborators (Scott Jackson, Phil McClean, and Jeremy Schmutz), for pre-publication access to release v2.1 of the Phaseolus vulgaris (common bean) genome and annotation. We thank Dr. Phil McClean for seeds of G19833. We are grateful for the help from Nicholas Kaczmar for planting and harvesting, as well as staff of Cornell’s Guterman Bioclimatic Laboratory for greenhouse management.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10722_2019_823_MOESM1_ESM.pdf (922 kb)
ESM_1. Supplemental Fig. S1 Visual examples of the seed coat pattern classification used in this study. (PDF 922 kb)
10722_2019_823_MOESM2_ESM.pdf (678 kb)
ESM_2. Supplemental Fig. S2 Visual examples of the seed coat pattern for all six accessions classified as “Anasazi.” (PDF 677 kb)
10722_2019_823_MOESM3_ESM.pdf (89 kb)
ESM_3. Supplemental Fig. S3 Schematic overview of the SNP filtering pipeline. SNP Set 0 was used to identify unintended sample duplicates. SNP Set I was used to separate accessions into Andean and Mesoamerican groups. SNP Set II was used for GWAS. SNP Set III was used for population genetics analysis. (PDF 89 kb)
10722_2019_823_MOESM4_ESM.pdf (8 kb)
ESM_4. Supplemental Fig. S4 Principal component analysis of SNP Set I. Mesoamerican NPGS and Andean NPGS classifications are based on a priori annotation of NPGS accessions. Mesoamerican NSS and Andean NSS classifications are based on K-means clustering (K = 2) along PC1 with the respective group of NPGS accessions. (PDF 7 kb)
10722_2019_823_MOESM5_ESM.pdf (7 kb)
ESM_5. Supplemental Fig. S5 Principal component analysis of SNP Set II. Subpopulation labels are based on fastSTRUCTURE analysis of SNP Set III, with a subpopulation assignment criterion of Q ≥ 0.8. (PDF 6 kb)
10722_2019_823_MOESM6_ESM.pdf (43 kb)
ESM_6. Supplemental Fig. S6 Neighbour joining phylogenetic tree of the NS/S (SP1, SP2, SP3, SP mixed) and NPGS (NPGS Durango, NPGS Jalisco, NPGS Mesoamerica) Mesoamerican accessions, rooted using the reference genome accession (G19833, 8 samples, Andean genotype). NPGS accession PI 615391, used in both our study and Kwak and Gepts (2009), is indicated by the dotted line and label. Subpopulation labels are based on fastSTRUCTURE analysis of SNP Set III, with a subpopulation assignment criterion of Q ≥ 0.8. (PDF 42 kb)
10722_2019_823_MOESM7_ESM.pdf (111 kb)
ESM_7. Supplemental Fig. S7 GWAS results of three-class and binary coding of seed coat pattern traits. SNPs selected by the MLMM as significantly associated with the trait are shown in red, while the remaining SNPs are shown in black. (PDF 110 kb)
10722_2019_823_MOESM8_ESM.pdf (5 kb)
ESM_8. Supplemental Fig. S8 Linkage disequilibrium (LD) estimates between SNPs in the NS/S Mesoamerican population of 281 accessions. The distribution of SNPs at different percentile cutoffs are indicated by the labeled lines. Median LD is indicated by the solid line labelled 50%, which decays to background levels (r2 < 0.1) at a physical distances beyond 128 Kb. (PDF 4 kb)
10722_2019_823_MOESM9_ESM.pdf (12 kb)
ESM_9. Supplemental Fig. S9 Histograms of latitude distributions for accessions used in this study and three previous studies. The red color indicates accessions collected from Mexico. (PDF 12 kb)
10722_2019_823_MOESM10_ESM.pdf (9 kb)
ESM_10. Supplemental Fig. S10 Subpopulation composition of the six accessions with Anasazi phenotypes using fastSTRUCTURE results. Three accessions (15DW320, 15DW324, 15DW306) are assigned to SP1 at Q ≥ 0.5, but classified as mixed at Q ≥ 0.8. (PDF 9 kb)
10722_2019_823_MOESM11_ESM.csv (30 kb)
ESM_11. Supplemental Table S1 The complete list of 375 accessions included in this study and relevant metadata. The “Group ID” column indicates whether or not an accession was part of an unintended sample duplicate group, and the “Choice” column indicates whether or not an accession was selected as a representative for its unintended sample duplicate group. The NS/S-assigned accession number, catalog number, common name, and phenotype description are reported for accessions sourced from NS/S. For NPGS sourced accessions, the data refers to annotation from the GRIN database. The race of each NPGS accession was inferred using the documented seed type and morphology description with criteria from Singh et al. (1991). (CSV 29 kb)
10722_2019_823_MOESM12_ESM.csv (13 kb)
ESM_12. Supplemental Table S2 Accession geographical provenance and seed coat pattern trait encoding for the NS/S accessions of Mesoamerican origin. The last column indicates the corresponding seed coat pattern and color descriptors according to IBPGR (1982) and Kornerup (1967). (CSV 12 kb)
10722_2019_823_MOESM13_ESM.csv (0 kb)
ESM_13. Supplemental Table S3 Summary of the four SNP sets used in this study. (CSV 0 kb)
10722_2019_823_MOESM14_ESM.csv (0 kb)
ESM_14. Supplemental Table S4FST results of all pairwise comparisons between the three subpopulations within the NS/S Mesoamerican population. (CSV 0 kb)
10722_2019_823_MOESM15_ESM.csv (1 kb)
ESM_15. Supplemental Table S5 BLAST results for STS marker sequences for a priori seed coat color and pattern genes. Putative genomic locations of seven out of the 11 STS markers described in Table 2 of McClean et al. (2002) are presented. (CSV 1 kb)
10722_2019_823_MOESM16_ESM.csv (2 kb)
ESM_16. Supplemental Table S6 Seed coat pattern trait GWAS results and genomic location information for MLMM selected SNPs. (CSV 1 kb)
10722_2019_823_MOESM17_ESM.csv (45 kb)
ESM_17. Supplemental Table S7 All annotated genes within 128 Kb of the MLMM selected SNPs reported in Supplemental Table S6. (CSV 45 kb)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Plant Breeding and Genetics Section, School of Integrative Plant ScienceCornell UniversityIthacaUSA
  2. 2.Native Seeds/SEARCHTucsonUSA
  3. 3.USDA-ARS Tropical Agriculture Research StationMayagüezUSA

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