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Fatty acid composition and oil content of seeds from perilla (Perilla frutescens (L.) var. frutescens) germplasm of Republic of Korea

  • Hyun Uk KimEmail author
  • Kyeong-Ryeol Lee
  • Inhwa Jeon
  • Ha Eun Jung
  • Jae Bok Heo
  • Tae-Yun Kim
  • Grace Q. Chen
Research Article
  • 32 Downloads

Abstract

Perilla (Perilla frutescens (L.) var. frutescens) is an oilseed crop that produces a large amount of α-linolenic acid (ALA, 18:3), an ω-3 fatty acid in seeds. Perilla is also a fragrant leafy herb with great health benefits. To develop value-added perilla crop, we surveyed the fatty acid composition and oil content of 87 germplasms accessions maintained at Republic of Korea. Our results show that ALA is the major fatty acid, ranging from 47 to 64%, followed by linoleic acid (LA, 18:2, an ω-6 fatty acid) from 10 to 24% and the oleic acid (OA, 18:1, an ω-9 fatty acid) from 9 to 20%. The seed oil content among accessions ranges from 17 to 42.7%. To access if the different fatty acid profile and oil content among germplasms are associated with changes in key genes, we compare the sequences encoding fatty acid desaturase 2 (FAD2) and fatty acid desaturase 3 (FAD3) responsible for LA and ALA synthesis, respectively. Besides, we examine the sequence of diacylglycerol acyltransferase 1 (DGAT1), a key enzyme in determining oil content. We do not find major changes in FAD2 or FAD3 associated with different fatty acid profiles. We discover a change in DGAT1 that might associate a low oil content in seeds.

Keywords

Perilla germplasm ω-3 Fatty acid ω-9 Fatty acid α-Linolenic acid Oleic acid 

Notes

Acknowledgements

This study was conducted with the support of the Research Program for Agricultural Science & Technology Development (Project No. PJ01257102), the National Institute of Agricultural Science, and the Next-Generation BioGreen 21 Program (SSAC, Grant No. PJ013185), Rural Development Administration, Republic of Korea, The Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry, and Fisheries (IPET) (116079-03 and 316087-4), Republic of Korea, and the MidCareer Researcher Program of the National Research Foundation of Korea (NRF-2017R1A2B4007096). This study was supported in part by the U.S. Department of Agriculture Current Research Information System Project 2030-21410-020-00D (to Grace Q. Chen). USDA is an equal opportunity provider and employer. Mention of a specific product name by the U.S. Department of Agriculture does not constitute an endorsement and does not imply a recommendation over other suitable products.

Author contributions

HUK, KRL, IJ, HEJ, JBH, and TYK performed the experiments; HUK, KRL, and GQC analyzed the data; and HUK, KRL, and GQC wrote the paper. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10722_2019_803_MOESM1_ESM.pdf (3.2 mb)
Supplementary material 1 (PDF 3321 kb)

References

  1. Asif M (2011) Health effects of omega-3,6,9 fatty acids: Perilla frutescens is a good example of plant oils. Orient Pharm Exp Med 11:51–59CrossRefGoogle Scholar
  2. Brenner DM (1993) Perilla: botany, uses and genetic resources. In: Janick J, Simon JE (eds) New crops. Wiley, New York, pp 322–328Google Scholar
  3. Deng Y-m, Xie Q-m, Zhang S-j, Yao H-y, Zhang H (2007) Anti-asthmatic effects of perilla seed oil in the guinea pig in vitro and in vivo. Planta Med 73:53–58CrossRefGoogle Scholar
  4. Deng KP, Fan YX, Ma TW, Wang Z, TanTai WJ, Nie HT, Guo YX, Yu XQ, Sun LW, Wang F (2018) Carcass traits, meat quality, antioxidant status and antioxidant gene expression in muscle and liver of Hu lambs fed perilla seed. J Anim Physiol Anim Nutr 102:e828–e837CrossRefGoogle Scholar
  5. Dyer JM, Stymne S, Green AG, Carlsson AS (2008) High-value oils from plants. Plant J 54:640–655CrossRefGoogle Scholar
  6. Eckert GP, Franke C, Nöldner M, Rau O, Wurglics M, Schubert-Zsilavecz M, Müller WE (2010) Plant derived omega-3-fatty acids protect mitochondrial function in the brain. Pharmacol Res 61:234–241CrossRefGoogle Scholar
  7. Graef G, LaVallee BJ, Tenopir P, Tat M, Schweiger B, Kinney AJ, Van Gerpen JH, Clemente TE (2009) A high-oleic-acid and low-palmitic-acid soybean: agronomic performance and evaluation as a feedstock for biodiesel. Plant Biotechnol J 7:411–421CrossRefGoogle Scholar
  8. Honda G, Yuba A, Kojima T, Tabata M (1994) Chemotaxonomic and cytogenetic studies on Perilla frutescens var. citriodora (‘Lemon Egoma’). Nat Med 48:185–190Google Scholar
  9. Ito M, Honda G (1996) A taxonomic study of Japanese wild Perilla (Labiatae). J Phytogeogr Taxon 44:43–52Google Scholar
  10. Ito M, Kato H, Oka Y, Honda G (1998) Phylogenetic analysis of Japanese Perilla species by using DNA polymorphisms. Nat Med 52:248–252Google Scholar
  11. Jako C, Kumar A, Wei Y, Zou J, Barton DL, Giblin EM, Covello PS, Taylor DC (2001) Seed-specific over-expression of an Arabidopsis cDNA encoding a diacylglycerol acyltransferase enhances seed oil content and seed weight. Plant Physiol 126:861–874CrossRefGoogle Scholar
  12. James MJ, Gibson RA, Cleland LG (2000) Dietary polyunsaturated fatty acids and inflammatory mediator production. Am J Clin Nutr 71:343s–348sCrossRefGoogle Scholar
  13. Jiang C, Shi J, Li R, Long Y, Wang H, Li D, Zhao J, Meng J (2014) Quantitative trait loci that control the oil content variation of rapeseed (Brassica napus L.). Theor Appl Genet 127:957–968CrossRefGoogle Scholar
  14. Katavic V, Reed DW, Taylor DC, Giblin EM, Barton DL, Zou J, MacKenzie SL, Covello PS, Kunst L (1995) Alteration of seed fatty acid composition by an ethyl methanesulfonate-induced mutation in Arabidopsis thaliana affecting diacylglycerol acyltransferase activity. Plant Physiol 108:399–409CrossRefGoogle Scholar
  15. Kim HU, Lee K-R, Shim D, Lee JH, Chen GQ, Hwang S (2016) Transcriptome analysis and identification of genes associated with ω-3 fatty acid biosynthesis in Perilla frutescens (L.) var. frutescens. BMC Genom 17:474CrossRefGoogle Scholar
  16. Lee H-C, Ko H-K, Huang BETG, Chu Y-H, Huang S-Y (2014) Antidepressant-like effects of Perilla frutescens seed oil during a forced swimming test. Food Funct 5:990–996CrossRefGoogle Scholar
  17. Lee J, Rodriguez JP, Kim YJ, Lee MH, Cho EJ, Lee S (2016a) Fatty acid content in Perilla cultivars and commercial oils determined by GC analysis. Nat Prod Sci 22:259–262CrossRefGoogle Scholar
  18. Lee K-R, Lee Y, Kim E-H, Lee S-B, Roh KH, Kim J-B, Kang H-C, Kim HU (2016b) Functional identification of oleate 12-desaturase and ω-3 fatty acid desaturase genes from Perilla frutescens var. frutescens. Plant Cell Rep 35:2523–2537CrossRefGoogle Scholar
  19. Li C, Miao H, Wei L, Zhang T, Han X, Zhang H (2014) Association mapping of seed oil and protein content in Sesamum indicum L. using SSR markers. PLoS ONE 9:e105757CrossRefGoogle Scholar
  20. Liao B, Hao Y, Lu J, Bai H, Guan L, Zhang T (2018) Transcriptomic analysis of Perilla frutescens seed to insight into the biosynthesis and metabolic of unsaturated fatty acids. BMC Genom 19:213CrossRefGoogle Scholar
  21. Lung S-C, Weselake RJ (2006) Diacylglycerol acyltransferase: a key mediator of plant triacylglycerol synthesis. Lipids 41:1073–1088CrossRefGoogle Scholar
  22. Müller-Waldeck F, Sitzmann J, Schnitzler WH, Graßmann J (2010) Determination of toxic perilla ketone, secondary plant metabolites and antioxidative capacity in five Perilla frutescens L. varieties. Food Chem Toxicol 48:264–270CrossRefGoogle Scholar
  23. Nitta M, Lee JK, Ohnishi O (2003) Asian Perilla crops and their weedy forms: their cultivation, utilization and genetic relationships. Econ Bot 57:245–253CrossRefGoogle Scholar
  24. Nitta M, Lee JK, Kang CW, Katsuta M, Yasumoto S, Liu D, Nagamine T, Ohnishi O (2005) The distribution of Perilla species. Genet Resour Crop Evol 52:797–804CrossRefGoogle Scholar
  25. Okuley J, Lightner J, Feldmann K, Yadav N, Lark E, Browse J (1994) Arabidopsis FAD2 gene encodes the enzyme that is essential for polyunsaturated lipid synthesis. Plant Cell 6:147–158Google Scholar
  26. Okuno M, Kajiwara K, Imai S, Kobayashi T, Honma N, Maki T, Suruga K, Goda T, Takase S, Muto Y, Moriwaki H (1997) Perilla oil prevents the excessive growth of visceral adipose tissue in rats by down-regulating adipocyte differentiation. J Nutr 127:1752–1757CrossRefGoogle Scholar
  27. Routaboul J-M, Benning C, Bechtold N, Caboche M, Lepiniec L (1999) The TAG1 locus of Arabidopsis encodes for a diacylglycerol acyltransferase. Plant Physiol Biochem 37:831–840CrossRefGoogle Scholar
  28. Sakurai K, Asahi K, Kanesaki Y, Hayashi Y, Asai J, Yuza T, Watanabe K, Katoh T, Watanabe T (2011) Dietary Perilla seed oil supplement increases plasma omega-3 polyunsaturated fatty acids and ameliorates immunoglobulin A nephropathy in high immunoglobulin A strain of ddY mice. Nephron Exp Nephrol 119:e33–e39CrossRefGoogle Scholar
  29. Shin H-S, Kim S-W (1994) Lipid composition of perilla seed. J Am Oil Chem Soc 71:619–622CrossRefGoogle Scholar
  30. Simopoulos AP (1991) Omega-3 fatty acids in health and disease and in growth and development. Am J Clin Nutr 54:438–463CrossRefGoogle Scholar
  31. Smith MA, Moon H, Chowrira G, Kunst L (2003) Heterologous expression of a fatty acid hydroxylase gene in developing seeds of Arabidopsis thaliana. Planta 217:507–516CrossRefGoogle Scholar
  32. Warner K, Knowlton S (1997) Frying quality and oxidative stability of high-oleic corn oils. J Am Oil Chem Soc 74:1317–1322CrossRefGoogle Scholar
  33. Wijendran V, Hayes KC (2004) Dietary n-6 and n-3 fatty acid balance and cardiovascular health. Ann Rev Nutr 24:597–615CrossRefGoogle Scholar
  34. Yamazaki M, Shibata M, Nishiyama Y, Springob K, Kitayama M, Shimada N, Aoki T, Ayabe S, Saito K (2008) Differential gene expression profiles of red and green forms of Perilla frutescens leading to comprehensive identification of anthocyanin biosynthetic genes. FEBS J 275:3494–3502CrossRefGoogle Scholar
  35. Zhang T, Song C, Song L, Shang Z, Yang S, Zhang D, Sun W, Shen Q, Zhao D (2017) RNA sequencing and coexpression analysis reveal key genes involved in α-linolenic acid biosynthesis in Perilla frutescens seed. Int J Mol Sci 18:2433CrossRefGoogle Scholar
  36. Zheng P, Allen WB, Roesler K, Williams ME, Zhang S, Li J, Glassman K, Ranch J, Nubel D, Solawetz W, Bhattramakki D, Llaca V, Deschamps S, Zhong GY, Tarczynski MC, Shen B (2008) A phenylalanine in DGAT is a key determinant of oil content and composition in maize. Nat Genet 40:367–372CrossRefGoogle Scholar
  37. Zou J, Wei Y, Jako C, Kumar A, Selvaraj G, Taylor DC (1999) The Arabidopsis thaliana TAG1 mutant has a mutation in a diacylglycerol acyltransferase gene. Plant J 19:645–653CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Bioindustry and Bioresource EngineeringSejong UniversitySeoulRepublic of Korea
  2. 2.Department of Agricultural BiotechnologyNational Institute of Agricultural Sciences, Rural Development AdministrationJeonjuRepublic of Korea
  3. 3.Department of Molecular Genetic BiotechnologyDong-A UniversityBusanRepublic of Korea
  4. 4.Asia Seed CompanyIcheonRepublic of Korea
  5. 5.Western Regional Research CenterAgricultural Research Service, U.S. Department of AgricultureAlbanyUSA

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