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Genetic Resources and Crop Evolution

, Volume 66, Issue 7, pp 1443–1457 | Cite as

Conservation and differentiation of polyphenol oxidase (PPO) gene introns in Triticum and Aegilops tauschii Coss.

  • Yun Fang LiEmail author
  • Yu Wu
  • Chun Yan Zhang
  • Lei Zhang
  • Ze Hou Liu
  • Chi Hong Zhang
Research Article
  • 111 Downloads

Abstract

Two genes (PPO1 and PPO2), located in the homoeologous chromosome group 2 of common wheat, play an important role in controlling the kernel polyphenol oxidase acitivity, which influence indirectly the time-dependent darkening and discoloration in Asian noodles and other wheat-based products. In the present study, intron sequences of PPO genes (PPO1 and PPO2) in Triticum urartu Tumanian ex Gandilyan, T. monococcum L. and Aegilops tauschii Coss., together with NCBI retrieved wheat PPO sequnces in different ploidy levels, were systematically investigated. The results highlighted that PPO intron sequences are conserved at lower classification levels (species/subspecies level), and the allopolyploidization process of common wheat has little effect on them. Sequence differences in PPO gene introns may occur long before the differentiation of the A and D genome of T. urartu and Ae. tauschii, respectively. It’s interesting that different PPO intron regions vary into two different intron splicing rule within one same gene sequence: intron 1 is all fitted to the “GT-AG” splicing rule while intron 2 is the “GC-AG”. In addition, the phylogenetic analysis showed the obvious differences existed in materials from different geographical distribution in T. monococcum ssp. aegilopoies, which suggest that some of them had close genetic relationship with T. urartu. These findings contribute to the application of PPO gene introns in favorable allele screening, functional molecular marker development, and phylogenetic analysis.

Keywords

Polyphenol oxidase Intron Allopolyploidization Triticum urartu Triticum monococcum Aegilops taushcii 

Notes

Acknowledgements

We thank Professor Bihua Wu, Dr. Lianquan Zhang of Sichuan Agricultural University for providing their valuable synthetic hexaploid wheat and other materials collected.

Funding

This project was partially funded by Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences (Class A, No. XDA08030106).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

References

  1. Anderson JV, Patrick Fuerst E, Hurkman WJ, Vensel WH, Morris CF (2006) Biochemical and genetic characterization of wheat (Triticum spp.) kernel polyphenol oxidases. J Cereal Sci 44:353–367CrossRefGoogle Scholar
  2. Baik B, Czuchajowska Z, Pomeranz Y (1995) Discoloration of dough for oriental noodles. Cereal Chem 72:198–204Google Scholar
  3. Beecher B, Skinner DZ (2011) Molecular cloning and expression analysis of multiple polyphenol oxidase genes in developing wheat (Triticum aestivum) kernels. J Cereal Sci 53:371–378CrossRefGoogle Scholar
  4. Beecher BS, Carter AH, See DR (2012) Genetic mapping of new seed-expressed polyphenol oxidase genes in wheat (Triticum aestivum L.). Theor Appl Genet 124:1463–1473CrossRefGoogle Scholar
  5. Brown JWS, Smith P, Simpson CG (1996) Arabidopsis consensus intron sequences. Plant Mol Biol 32:531–535CrossRefGoogle Scholar
  6. Burset M, Seledtsov IA, Solovyev VV (2000) Analysis of canonical and non-canonical splice sites in mammalian genomes. Nucleic Acids Res 28:4364–4375CrossRefGoogle Scholar
  7. Chang C, Zhang HP, Xu J, You MS, Li BY, Liu GT (2007) Variation in two PPO genes associated with polyphenol oxidase activity in seeds of common wheat. Euphytica 154:181–193CrossRefGoogle Scholar
  8. Demeke T, Morris C (2002) Molecular characterization of wheat polyphenol oxidase (PPO). Theor Appl Genet 104:813–818CrossRefGoogle Scholar
  9. Dvořák J, Terlizzi Pd, Zhang HB, Resta P (1993) The evolution of polyploid wheats: identification of the a genome donor species. Genome 36:21–31CrossRefGoogle Scholar
  10. Fedorova L, Fedorov A (2003) Introns in gene evolution. Genetica 118:123–131CrossRefGoogle Scholar
  11. Fuerst EP, Anderson JV, Morris CF (2006) Delineating the role of polyphenol oxidase in the darkening of alkaline wheat noodles. J Agric Food Chem 54:2378–2384CrossRefGoogle Scholar
  12. Fuerst EP, Xu SS, Beecher B (2008) Genetic characterization of kernel polyphenol oxidases in wheat and related species. J Cereal Sci 48:359–368CrossRefGoogle Scholar
  13. He XY, He ZH, Zhang LP, Sun DJ, Morris CF, Fuerst EP, Xia XC (2007) Allelic variation of polyphenol oxidase (PPO) genes located on chromosomes 2A and 2D and development of functional markers for the PPO genes in common wheat. Theor Appl Genet 115:47–58CrossRefGoogle Scholar
  14. He XY, He ZH, Morris CF, Xia XC (2009) Cloning and phylogenetic analysis of polyphenol oxidase genes in common wheat and related species. J Agric Food Chem 56:311–321Google Scholar
  15. Heun M, Schäferpregl R, Klawan D, Castagna R, Accerbi M, Borghi B, Salamini F (1997) Site of einkorn wheat domestication identified by DNA fingerprinting. Science 278:1312–1314CrossRefGoogle Scholar
  16. Irimia M (2008) Spliceosomal introns as tools for genomic and evolutionary analysis. Nucleic Acids Res 36:1703–1712CrossRefGoogle Scholar
  17. Jimenez M, Dubcovsky J (1999) Chromosome location of genes affecting polyphenol oxidase activity in seeds of common and durum wheat. Plant Breed 118:395–398CrossRefGoogle Scholar
  18. Johnson BL, Dhaliwal HS (1976) Reproductive isolation of Triticum boeoticum and Triticum urartu and the origin of the tetraploid wheats. Am J Bot 63:1088–1094CrossRefGoogle Scholar
  19. Jukanti AK, Bruckner PL, Fischer AM (2004) Evaluation of wheat polyphenol oxidase genes. Cereal Chem 81:481–485CrossRefGoogle Scholar
  20. Kilian B, Özkan H, Walther A, Kohl J, Dagan T, Salamini F, Martin W (2007) Molecular diversity at 18 loci in 321 wild and 92 domesticate lines reveal no reduction of nucleotide diversity during Triticum monococcum (Einkorn) domestication: implications for the origin of agriculture. Mol Biol Evol 24:2657–2668CrossRefGoogle Scholar
  21. Le Hir H, Nott A, Moore MJ (2003) How introns influence and enhance eukaryotic gene expression. Trends Biochem Sci 28:215–220CrossRefGoogle Scholar
  22. Lev-Yadun S, Gopher A, Abbo S (2000) The cradle of agriculture. Science 288:1602–1603CrossRefGoogle Scholar
  23. Mares DJ, Campbell AW (2001) Mapping components of flour and noodle colour in Australian wheat. Crop Pasture Sci 52:1297–1309CrossRefGoogle Scholar
  24. Martin JM, Berg JE, Hofer P, Kephart KD, Nash D, Bruckner PL (2011) Allelic variation of polyphenol oxidase genes impacts on Chinese raw noodle color. J Cereal Sci 54:387–394CrossRefGoogle Scholar
  25. Massa AN, Beecher B, Morris CF (2007) Polyphenol oxidase (PPO) in wheat and wild relatives: molecular evidence for a multigene family. Theor Appl Genet 114:1239–1247CrossRefGoogle Scholar
  26. Mccallum JA, Walker JRL (1990) O-diphenol oxidase activity, phenolic content and colour of New Zealand wheats, flours and milling streams. J Cereal Sci 12:83–96CrossRefGoogle Scholar
  27. Mousavifard SS, Saeidi H, Rahiminejad MR, Shamsadini M (2014) Molecular analysis of diversity of diploid Triticum species in Iran using ISSR markers. Genet Resour Crop Evol 62:387–394CrossRefGoogle Scholar
  28. Nasernakhaei F, Rahiminejad MR, Saeidi H, Tavassoli M (2014) Genetic structure and diversity of Triticum monococcum ssp. aegilopoides and T. urartu in Iran. Plant Genet Resour-Charact Util 13:1–8CrossRefGoogle Scholar
  29. Parada GE, Munita R, Cerda CA, Gysling K (2014) A comprehensive survey of non-canonical splice sites in the human transcriptome. Nucleic Acids Res 42:10564–10578CrossRefGoogle Scholar
  30. Patel AA, Matthew MC, Steitz JA (2002) The splicing of U12-type introns can be a rate-limiting step in gene expression. EMBO J 21:3804–3815CrossRefGoogle Scholar
  31. Petersen G, Seberg O, Yde M, Berthelsen K (2006) Phylogenetic relationships of Triticum and Aegilops and evidence for the origin of the A, B, and D genomes of common wheat (Triticum aestivum). Mol Biol Evol 39:70–82Google Scholar
  32. Raman R (2007) Functional gene markers for polyphenol oxidase locus in bread wheat. Mol Breed 19:315–328CrossRefGoogle Scholar
  33. Raman R, Raman H, Johnstone K, Lisle C, Smith A, Matin P, Allen H (2005) Genetic and in silico comparative mapping of the polyphenol oxidase gene in bread wheat (Triticum aestivum L.). Funct Integr Genomics 5:185–200CrossRefGoogle Scholar
  34. Raskina O, Belyayev A, Nevo E (2004) Quantum speciation in Aegilops: molecular cytogenetic evidence from rDNA cluster variability in natural populations. Proc Natl Acad Sci USA 101:14818–14823CrossRefGoogle Scholar
  35. Rose AB (2008) Intron-mediated regulation of gene expression. Curr Top Microbiol Immunol 326:277–290Google Scholar
  36. Si HQ, Zhou ZL, Wang XB, Ma CX (2012) A novel molecular marker for the polyphenol oxidase gene located on chromosome 2B in common wheat. Mol Breed 30:1371–1378CrossRefGoogle Scholar
  37. Simeone R, Pasqualone A, Clodoveo ML, Blanco A (2002) Genetic mapping of polyphenol oxidase in tetraploid wheat. Curr Top Microbiol Immunol 7:763–769Google Scholar
  38. Sparks ME, Brendel V (2005) Incorporation of splice site probability models for non-canonical introns improves gene structure prediction in plants. Bioinformatics 21(Suppl 3):iii20–iii30CrossRefGoogle Scholar
  39. Sun DJ, He ZH, Xia XC, Zhang LP, Morris CF, Appels R, Ma WJ, Wang H (2005) A novel STS marker for polyphenol oxidase activity in bread wheat. Mol Breed 16:209–218CrossRefGoogle Scholar
  40. Sun Y, He Z, Ma W, Xia X (2011) Alternative splicing in the coding region of Ppo-A1 directly influences the polyphenol oxidase activity in common wheat (Triticum aestivum L.). Funct Integr Genomics 11:85–93CrossRefGoogle Scholar
  41. van Slageren MW (1994) Wild wheats: a monograph of Aegilops L. and Amblyopyrum (Jaub. & Spach) Eig (Poaceae). Wageningen Agricultural University papers, Wageningen, the NetherlandsGoogle Scholar
  42. Waines JG, Barnhart D (1992) Biosystematic research in Aegilops and Triticum. Hereditas 116:207–212CrossRefGoogle Scholar
  43. Wang XB, Ma CX, Si HQ, Qiao YQ, Chang C, He XF, Xia YX (2009) Gene markers for grain polyphenol oxidase activity in common wheat. Mol Breed 23:163–170CrossRefGoogle Scholar
  44. Watanabe N, Akond AM, Nachit MM (2006) Genetic mapping of the gene affecting polyphenol oxidase activity in tetraploid durum wheat. J Appl Genet 47:201–205CrossRefGoogle Scholar
  45. Willcox G (2005) The distribution, natural habitats and availability of wild cereals in relation to their domestication in the Near East: multiple events, multiple centres. Vege Hist Archaeobot 14:534–541CrossRefGoogle Scholar
  46. Zohary D, Hopf M (2000) Domestication of plants in the old world, 3rd edn. Oxford University Press, New YorkGoogle Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Yun Fang Li
    • 1
    Email author
  • Yu Wu
    • 1
  • Chun Yan Zhang
    • 1
  • Lei Zhang
    • 1
  • Ze Hou Liu
    • 1
  • Chi Hong Zhang
    • 1
  1. 1.Department of Agriculture and BiotechnologyChengdu Institute of Biology, Chinese Academy of SciencesChengduChina

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