Germplasm collection, genetic diversity and on-farm conservation of cowpea [Vigna unguiculata (L.) Walp.] landraces from Apulia region (southern Italy)
- 148 Downloads
Cowpea [Vigna unguiculata (L.) Walp.] is a major tropical pulse in a number of countries and especially in sub-Saharan Africa where this species was domesticated. Although as niche product, a remarkable number of cowpea landraces are cultivated in the Mediterranean basin, primarily for human consumption of the grains and fresh pods. In this study, within the frame of a regional project devoted to the safeguard and management of local horticultural genetic resources, some germplasm explorations were carried out in Apulia region (southern Italy) to collect, among other crops, cowpea landraces still present in the area and promote on-farm conservation of this traditional material. A second objective was to investigate the efficiency of SSR markers in detecting genetic polymorphism in cowpea landraces from a restricted geographical area. In total, 13 samples of V. unguiculata were gathered, 12 of cultivar group unguiculata and 1 of cultivar group sesquipedalis. Genetic diversity and phylogenetic relationships among 13 Apulian accessions, 9 samples from various provenances and 1 var. spontanea accession were evaluated using SSR molecular markers. A set of 19 SSR primer pairs successfully identified closely related accessions and were able to separate the different cultivar groups of V. unguiculata.
KeywordsCowpea Genetic diversity Landraces Molecular markers On-farm conservation SSR Vigna unguiculata
We thank Francesco P. Losavio and Giuseppe Sonnante for technical help in the material and data collection.
This research was funded by the project BiodiverSO – PSR Puglia 2007-2013 Mis. 214/4 subaz. a) and by the project BiodiverSO – PSR Puglia 2014-2020 Mis.10.2.
- Boukar O, Bhattacharjee R, Fatokun C, Kumar PL, Gueye B (2013) Cowpea. In: Singh M, Upadhyaya HD, Bisht IS (eds) Genetic and genomic resources of grain legume improvement. Elsevier, London, pp 137–156. https://doi.org/10.1016/B978-0-12-397935-3.00006-2 CrossRefGoogle Scholar
- Carvalho M, Bebeli PJ, Pereira G, Castro I, Egea-Gilabert C, Matos M, Lazaridi E, Duarte I, Lino-Neto T, Ntatsi G, Rodriguez M, Savvas D, Rosa E, Carnide V (2017a) European cowpea landraces for a more sustainable agriculture system and novel foods. J Sci Food Agric 97:4399–4407. https://doi.org/10.1002/jsfa.8378 CrossRefGoogle Scholar
- FAO (2016) http://www.fao.org/faostat/en/#data/QC. Accessed Sept 2018
- Gonçalves A, Goufo P, Barros A, Domínguez-Perles R, Trindade H, Rosa EA, Rodrigues M (2016) Cowpea (Vigna unguiculata L. Walp), a renewed multipurpose crop for a more sustainable agri-food system: nutritional advantages and constraints. J Sci Food Agric 96:2941–2951. https://doi.org/10.1002/jsfa.7644 CrossRefGoogle Scholar
- Hammer K, Laghetti G, Cifarelli S, Perrino P (1991) Collecting in northern Italy using the indicator-crop method. FAO/IBPGR Plant Genet Resour Newsl 86:39–40Google Scholar
- Laghetti G, Padulosi S, Hammer K, Cifarelli S, Perrino P (1990) Cowpea (Vigna unguiculata) [L.] Walp.) germplasm collection in southern Italy and preliminary evaluation. In: Ng NQ, Monti LM (eds) Cowpea genetic resources. Ibadan, IITA, pp 46–57Google Scholar
- Maxted N, Mabuza D, Moss H, Padulosi S, Jarvis A, Guarino L (2004) An ecogeographic study: African Vigna. Systematic and ecogeographic studies of crop genepools 10. International Plant Genetic Resources Institute (IPGRI), Rome, pp 1–468Google Scholar
- Ng NQ, Marechal R (1985) Cowpea taxonomy, origin and germplasm. In: Singh SR, Rachie KO (eds) Cowpea research, production, and utilization. Wiley, Chichester, pp 11–21Google Scholar
- Ogunkanmi LA, Ogundipe OT, Ng NQ, Fatokun CA (2008) Genetic diversity in wild relatives of cowpea (Vigna unguiculata) as revealed by simple sequence repeats (SSR) markers. J Food Agric Environ 6:263–268Google Scholar
- Xu P, Wu X, Wang B, Liu Y, Ehlers JD, Close TJ, Roberts PA, Diop N, Qin D, Hu T, Lu Z, Li G (2011) A SNP and SSR based genetic map of asparagus bean (Vigna unguiculata ssp. sesquipedalis) and comparison with the broader species. PLoS ONE 6:e15952. https://doi.org/10.1371/journal.pone.0015952 CrossRefGoogle Scholar