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Glycan structures and their recognition roles in the human blood group ABH/Ii, Lea, b, x, y and Sialyl Lea,x active cyst glycoproteins

  • Albert M. WuEmail author
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Abstract

Human ovarian cyst glycoproteins (HOC, cyst gps) isolated from pseudomucinous type of human ovarian cyst fluids is one of the richest and pioneer sources for studying biosynthesis, structures and functional roles of blood group ABH, Lea,b,x,y, sLea and sLex active glycoproteins. After 70+ years of exploration, four top highlights are shared. (i) an updated concept of glycotopes and their internal structures in cyst gps was composited; (ii) the unknown codes of new genes in secreted cyst gps were unlocked as Lex and Ley; (iii) recognition profiles of cyst glycans and a sialic acid-rich (18%) glycan with lectins and antibodies were shown. (iv) Co-expression of Blood Group A/ A-Leb/y and B/B-Leb/y active Glycotopes in the same glycan chains were isolated and illustrated. These are the most advanced achievements since 1980.

Keywords

Human ovarian cyst glycoproteins Human blood group, Lex, Ley, Sialyl Lea, and Sialyl Lex active glycotopes and key sugars Glycotopes Crytopes (the masked glycotopes) glycan (carbohydrate) binding protein Lectins 

Abbreviations

HOC or cyst gp

Human ovarian cyst glycoprotein

RF

Recognition factor

Lea, b, x, y

Lea,Leb, Lex, Ley

sLea, sLex

Sialyl Lea and Sialyl Lex

GBP(CBP)

Glycan (Carbohydrate) binding Protein

SA

Sialic acid

GP or gp

Glycoprotein

ELLSA and ELLSIA

Enzyme-linked lectinosorbent assay; and inhibition assay

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Notes

Acknowledgements

This work was supported by grants from the Chang Gung Medical Research plan (CMRPD No. 180482 and BMR 0008) Kwei-san, Tao-yuan, Taiwan, and the National Science Council (NSC 97-2628-B-182-002-MY3 and 97-2320-B-182-020-MY3) Taipei, Taiwan.

The author would like to thanks Drs. Khoo KH and Yu S. Y. for their long term contributions in the field of structural identification. Yang Z MD’s assistance to make the data analyzed and organized.

Compliance with ethical standards

Conflicts of interest

The authors declare that they have no conflicts of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

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Authors and Affiliations

  1. 1.Glycomics Research Laboratory, Institute of Molecular and Cellular Biology, College of MedicineChang-Gung UniversityKwei-sanTaiwan

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