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Gangliosides profiling in serum of breast cancer patient: GM3 as a potential diagnostic biomarker

  • Qinying Li
  • Mei Sun
  • Mingsheng Yu
  • Qianyun Fu
  • Hao Jiang
  • Guangli Yu
  • Guoyun LiEmail author
Original Article

Abstract

Gangliosides altered during the pathological conditions and particularly in cancers. Here, we aimed to profile the gangliosides in breast cancer serum and propose potential biomarkers. LC-FTMS method was first used to identify all the ganglioside species in serum, then LC-MS/MS-MRM method was employed to quantitate the levels of gangliosides in serum from healthy volunteers and patients with benign breast tumor or breast cancer. 49 ganglioside species were determined, including GM1, GM2, GM3, GD1, GD3 and GT1 species. Compared to healthy volunteers, the levels of GM1, GM2, GM3, GD1 and GD3 displayed a rising trend in breast cancer patients. In particular, as the major glycosphingolipid component, GM3 showed excellent diagnostic accuracy in cancer serum (AUC > 0.9). PCA profile of the GM3 species showed clear distinction between normal and cancer serum. What’s more, ROC curve proved great diagnostic accuracy of GM3 between cancer and benign serum. In addition, GM3 was discovered as a diagnostic marker to differentiate luminal B subtype from other subtypes. Furthermore, a positive correlation between GM3 and Ki-67 status of patients was identified. In conclusion, our results introduced the alteration patterns of serum gangliosides in breast cancer and suggested serum GM3 as a potential diagnostic biomarker in breast cancer diagnosis and luminal B subtype distinction.

Keywords

Breast cancer Biomarker Gangliosides GM3 Serum 

Abbreviations

LA

Luminal A subtype

LB

Luminal B subtype

HER

HER-2 overexpressing subtype

BS

Basal-like subtype

Normal

Healthy volunteers

Benign

Benign breast tumor patients

Cancer

Breast cancer patients

ROC

Receiver operator characteristics

PCA

Principal component analysis

MRM

Multiple reaction monitoring

LC-FTMS

Liquid chromatography-Fourier transform mass spectrometry.

Notes

Acknowledgments

This work was supported by Grants from National Natural Science Foundation of China (31600646), Natural Science Foundation of Shandong Province (ZR2016HB42), the Fundamental Research Funds for the Central Universities (201762002), Qingdao Basic and Applied Research Project (18-2-2-25-jch), National Science and Technology Major Project for Significant New Drugs Development (2018ZX09735004), NSFC-Shandong Joint Fund for Marine Science Research Centers (U1606403) and Taishan scholar project special funds (TS201511011).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

10719_2019_9885_MOESM1_ESM.pdf (480 kb)
ESM 1 (PDF 479 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Qinying Li
    • 1
  • Mei Sun
    • 2
  • Mingsheng Yu
    • 2
  • Qianyun Fu
    • 1
  • Hao Jiang
    • 1
    • 3
  • Guangli Yu
    • 1
    • 3
  • Guoyun Li
    • 1
    • 3
    Email author
  1. 1.Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Shandong Provincial Key Laboratory of Glycoscience and GlycotechnologyOcean University of ChinaQingdaoChina
  2. 2.Qingdao Municipal Hospital, The Affiliated Qingdao Municipal HospitalQingdao University Medical CollegeQingdaoChina
  3. 3.Laboratory for Marine Drugs and BioproductsQingdao National Laboratory for Marine Science and TechnologyQingdaoChina

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