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Glycoconjugate Journal

, Volume 36, Issue 4, pp 241–257 | Cite as

“Stuck on sugars – how carbohydrates regulate cell adhesion, recognition, and signaling”

  • Richard D. CummingsEmail author
Original Article

Abstract

We have explored the fundamental biological processes by which complex carbohydrates expressed on cellular glycoproteins and glycolipids and in secretions of cells promote cell adhesion and signaling. We have also explored processes by which animal pathogens, such as viruses, bacteria, and parasites adhere to glycans of animal cells and initiate disease. Glycans important in cell signaling and adhesion, such as key O-glycans, are essential for proper animal development and cellular differentiation, but they are also involved in many pathogenic processes, including inflammation, tumorigenesis and metastasis, and microbial and parasitic pathogenesis. The overall hypothesis guiding these studies is that glycoconjugates are recognized and bound by a growing class of proteins called glycan-binding proteins (GBPs or lectins) expressed by all types of cells. There is an incredible variety and diversity of GBPs in animal cells involved in binding N- and O-glycans, glycosphingolipids, and proteoglycan/glycosaminoglycans. We have specifically studied such molecular determinants recognized by selectins, galectins, and many other C-type lectins, involved in leukocyte recruitment to sites of inflammation in human tissues, lymphocyte trafficking, adhesion of human viruses to human cells, structure and immunogenicity of glycoproteins on the surfaces of human parasites. We have also explored the molecular basis of glycoconjugate biosynthesis by exploring the enzymes and molecular chaperones required for correct protein glycosylation. From these studies opportunities for translational biology have arisen, involving production of function-blocking antibodies, anti-glycan specific antibodies, and synthetic glycoconjugates, e.g. glycosulfopeptides, that specifically are recognized by GBPs. This invited short review is based in part on my presentation for the IGO Award 2019 given by the International Glycoconjugate Organization in Milan.

Keywords

Glycoproteins Glycomics Selectins Cosmc T-synthase Inflammation Parasites Glycobiology Antibodies Immunity 

Notes

Acknowledgements

I would like to thank Jamie Heimburg-Molinaro and Sandra Cummings for thoughtful reading and editing of this manuscript. The work of the author over the years has been supported by various funding agencies, including most recently NIH Grants R01AG062181, P41GM103694, and R01AI101982 to RDC, Gates Foundation OPP1152154, OPP1151840 to RDC, and support to RDC by the U.S. Department of Health and Human Services contract HHSN272201400004C (NIAID Centers of Excellence for Influenza Research and Surveillance).

Compliance with ethical standards

Conflict of interest

The author declares that he has no conflicts of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by the author.

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Authors and Affiliations

  1. 1.Beth Israel Deaconess Medical CenterHarvard Medical SchoolBostonUSA

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