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Acute exercise increases syndecan-1 and -4 serum concentrations

  • Sindre Lee
  • Svein O. Kolset
  • Kåre I. Birkeland
  • Christian A. Drevon
  • Trine M. ReineEmail author
Original Article
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Abstract

The effects of acute and long-term exercise on syndecans and the relationship to insulin sensitivity are not fully explored. We aimed to examine the effects of acute and 12 weeks of exercise on (1) serum levels of syndecan-1 and -4, (2) gene expression related to syndecan synthesis and modification in skeletal muscle and adipose tissue, and (3) the relationship to insulin sensitivity. Sedentary men with (n = 13) or without (n = 13) dysglycemia underwent two 45 min acute bicycle tests interspersed by 12 weeks of exercise intervention. Euglycemic hyperinsulinemic clamp and mRNA-sequencing of skeletal muscle and adipose tissue biopsies were performed before and after intervention. Serum syndecan-1 and -4 levels were quantified before, immediately after and 2 h after bicycling. Syndecan-1 and -4 serum concentrations increased in response to acute physical exercise. Baseline syndecan-4 but not syndecan-1 concentrations were higher in dysglycemic compared to normoglycemic men, and correlated to change in insulin sensitivity, but did not change during the 12 weeks exercise intervention. Only syndecan-4 was expressed in skeletal muscle and adipose tissue. Adipose tissue mRNA levels of transcripts affecting syndecan structure and shedding were upregulated in dysglycemia, and muscle mRNA responded to long-term physical activity. The increase in serum syndecan-1 and -4 due to acute exercise suggest increased syndecan shedding and disruption of glycocalyx in response to increased blood flow. The higher syndecan-4 baseline serum levels in dysglycemia, association to insulin sensitivity, and changes in mRNA transcripts may suggest syndecan-4 involvement in muscle and adipose tissue response to exercise.

Keywords

Exercise Syndecans Syndecan-shedding Endothelial glycocalyx Dysglycemia Insulin sensitivity 

Notes

Acknowledgements

This work was supported by grants from the Institute of Basic Medical Sciences, UiO, Johan Throne-Holst Foundation for Nutrition Research, Freia Medical Research Foundation, the “Functional Genomics” and “Infrastructure” programs of the Research Council of Norway and the Southeastern Regional Health Authorities.

We thank Anne Randi Enget, Ansgar Heck and Birgitte Nellemann for taking the biopsies, and Tor I Gloppen, Torstein Dalen, Håvard Moen, Marius A Dahl, Guro Grøthe, Egil Johansen, Katrine A Krog, Øyvind Skattebo, Daniel S Tangen, Kristoffer K Jensen, Hans K Stadheim, and Eirin Rise for conducting the human strength and endurance intervention (MyoGlu) under supervision of Professor Jørgen Jensen. Sequencing was performed by PhD Gregor Gilfilan at the Norwegian Sequencing Centre (www.sequencing.uio.no) supported by the Research Council of Norway and the South-eastern Regional Health Authorities.

Author’s contributions

SL analyzed and prepared the data. SL, CAD and TMR interpreted the data and wrote the manuscript. KIB and CAD designed, planned and received grant funding for MyoGlu. All authors reviewed and revised the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the reported research.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

10719_2019_9869_MOESM1_ESM.docx (63 kb)
ESM 1 (DOCX 63 kb)

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Nutrition, Institute of Basic Medical Sciences, Faculty of MedicineUniversity of OsloOsloNorway
  2. 2.Department of Endocrinology, Morbid Obesity and Preventive MedicineOslo University HospitalOsloNorway
  3. 3.Institute of Clinical Medicine, Faculty of medicineUniversity of OsloOsloNorway
  4. 4.Institute for Cancer Genetics and InformaticsOslo University HospitalOsloNorway

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